Colorimetric Technique for Antimony Detection Based on the Use of Gold Nanoparticles Conjugated with Poly-A Oligonucleotide

Abstract

A simple and rapid positive–negative colorimetric approach to determine the presence of antimony ions based on the use of gold nanoparticles conjugated with oligonucleotide (poly-A sequence) is developed. Colorimetric measurements reveal that the aggregates of modified gold nanoparticles were afforded after adding antimony ions, thus changing the solution color from pink to blue. The results of aptamer’s interaction on the gold nanoparticle surface with the target analyte can be detected either by photometry or by the naked eye. The realized assay provides rapid (2 min), sensitive (detection limit 10 ng/mL), specific, and precise (variation coefficient less than 3.8%) detection of antimony (III) in drinking water.

Preparasi Strip Imunokromatografi Koloid Emas untuk Deteksi Cepat Aeromonas hydrophila

“…Keterangan: A. Nilai absorbansi koloid emas dengan Koloid emas hasil sintesis dengan metode ini digunakan sebagai label pada strip imunokromatografi yang dikembangkan. Karakteristik koloid emas dengan puncak serapan pada panjang gelombang 525 nm dengan ukuran partikel 30 nm telah dilaporkan (Rahme et al, 2013;Zeng et al, 2014;Berlina et al, 2019) dan banyak peneliti menggunakan koloid emas dengan ukuran partikel tersebut sebagai label pada pembuatan strip imunokromatografi (Sun et al, 2012;Liu et al, 2014;Ariffin et al, 2020). Koloid emas dengan ukuran partikel 31,88 nm pada penelitian ini memberikan kestabilan yang baik ketika dikonjugasikan dengan jumlah optimum antibodi anti A. hydrophila.…”

Mercaptosuccinic-Acid-Functionalized Gold Nanoparticles for Highly Sensitive Colorimetric Sensing of Fe(III) Ions

“…The implementation of nanomaterials into the development of colorimetric systems makes it possible to improve the sensitivity of the determination of toxins, as well as the accuracy of the analysis. The most common substrate that is used in colorimetric analysis is metal nanoparticles, especially silver [24,25] and gold nanoparticles (AuNPs) [26][27][28], due to their controllable morphology, chemical properties, and strong surface plasmon resonance (SPR). The ability of AuNPs to change color in response to changes in particle size and interparticle space, which is recorded spectrophotometrically as a shift in the absorption peak, makes them an ideal colorimetric sensing probe [28,29].…”

“…The most common substrate that is used in colorimetric analysis is metal nanoparticles, especially silver [24,25] and gold nanoparticles (AuNPs) [26][27][28], due to their controllable morphology, chemical properties, and strong surface plasmon resonance (SPR). The ability of AuNPs to change color in response to changes in particle size and interparticle space, which is recorded spectrophotometrically as a shift in the absorption peak, makes them an ideal colorimetric sensing probe [28,29]. Previously described work [30] demonstrated the use of native citrate-stabilized gold nanoparticles for the simultaneous detection of several ions.…”