Colorimetric Immunoassay for Detection of Tumor Markers

Yin, Yuan; Cao, Ya; Xu, Yuanyuan; Li, Genxi

doi:10.3390/ijms11125077

Cited by 60 publications

(28 citation statements)

References 80 publications

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“…The most commonly used biofluid biomarkers for cancer detection are specific proteins circulating in human blood, such as the alpha fetoprotein for liver cancer; chromogranin A for neuroendocrine tumors, especially carcinoid tumors; nuclear matrix protein 22 for bladder cancer; and carbohydrate antigen 125 for ovarian cancer [58]. Most of these blood proteins among others, are usually detected by various calorimetric and fluorescence immunoassays [59]. More accurate techniques for cancer detection are biopsy, endoscopy and radio-imaging tests such as PET-CT (positron emission computed tomography-computer tomography) and MRI (magnetic resonance imaging) [59].…”

Section: Circulatory Mirnas In Common Cancersmentioning

confidence: 99%

“…Most of these blood proteins among others, are usually detected by various calorimetric and fluorescence immunoassays [59]. More accurate techniques for cancer detection are biopsy, endoscopy and radio-imaging tests such as PET-CT (positron emission computed tomography-computer tomography) and MRI (magnetic resonance imaging) [59]. Unfortunately, above mention diagnostic methods are not sufficient for cancer detection at early stages, also some of them quite expensive [59].…”

Section: Circulatory Mirnas In Common Cancersmentioning

confidence: 99%

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Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Kumar

Reddy

2016

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

241

181

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Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by memory loss, multiple cognitive abnormalities and intellectual impairments. Currently, there are no drugs or agents that can delay and/or prevent the progression of disease in elderly individuals, and there are no peripheral biomarkers that can detect AD early in its pathogenesis. Research has focused on identifying biomarkers for AD so that treatment can be begun as soon as possible in order to restrict or prevent intellectual impairments, memory loss, and other cognitive abnormalities that are associated with the disease. One such potential biomarker is microRNAs that are found in circulatory biofluids, such as blood and blood components, serum and plasma. Blood and blood components are primary sources where miRNAs are released in either cell-free form and then bind to protein components, or are in an encapsulated form with microvesicle particles. Exosomal miRNAs are known to be stable in biofluids and can be detected by high throughput techniques, like microarray and RNA sequencing. In AD brain, enriched miRNAs encapsulated with exosomes crosses the blood brain barrier and secreted in the CSF and blood circulations. This review summarizes recent studies that have identified miRNAs in the blood, serum, plasma, exosomes, cerebral spinal fluids, and extracellular fluids as potential biomarkers of AD. Recent research has revealed only six miRNAs–miR-9, miR-125b, miR-146a, miR-181c, let-7g-5p, and miR-191-5p – that were reported by multiple investigators. Some studies analyzed the diagnostic potential of these six miRNAs through receiver operating curve analysis which indicates the significant area-under-curve values in different biofluid samples. miR-191-5p was found to have the maximum area-under-curve value (0.95) only in plasma and serum samples while smaller area-under-curve values were found for miR-125, miR-181c, miR-191-5p, miR-146a, and miR-9. This article shortlisted the promising miRNA candidates and discussed their diagnostic properties and cellular functions in order to search for potential biomarker for AD.

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Section: Circulatory Mirnas In Common Cancersmentioning

confidence: 99%

Section: Circulatory Mirnas In Common Cancersmentioning

confidence: 99%

Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Kumar

Reddy

2016

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

241

181

View full text Add to dashboard Cite

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“…8 These traditional label-based immunoassays are often complicated, timeconsuming, with the high cost of labeling antibodies. As such, development of label-free immunodetection techniques is highly desired to reduce the cost of detection systems as well as to simplify cancer screening procedures.…”

mentioning

confidence: 99%

Label-free immunodetection of the cancer biomarker CA125 using high-Δn liquid crystals

Lee

et al. 2014

J. Biomed. Opt

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Abstract. A label-free and array-based optical liquid-crystal (LC) immunodetection technique for the detection of CA125 antigen, a protein biomarker most frequently used for ovarian cancer detection, was demonstrated with a nematic LC with larger birefringence (Δn) to promote sensitivity in detecting biomolecules. The LC-based immunodetection offers an alternative and sensitive approach for the detection of biomarker proteins, with the potential of replacing conventional immunoassays used in biochemical studies and clinical laboratories. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Furthermore modified AgNPs with some specific linking groups (active chemical carboxyl and amino groups) for further covalently binding to antibodies or other biomolecules are commercially available 7,8 . New techniques that use specific modified AgNPs has several advantages such as rapid and safe approach to separate the target from other species, preventing the interference of non-specific proteins in immunoassays, easily detectable signals, simplifying the designed detection system, greater sensitivity and accuracy 9 . Despite on the great breakthrough of nanotechnology, modern sensing immunoassays has several disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Improving of enzyme immunoassay for detection and quantification of the target molecules using silver nanoparticles

Syrvatka

Slyvchuk

Rozgoni

et al. 2014

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI

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Modern routine enzyme immunoassays for detection and quantification of biomolecules have several disadvantages such as high cost, insufficient sensitivity, complexity and long-term execution. The surface plasmon resonance of silver nanoparticles gives reasons of creating new in the basis of simple, highly sensitive and low cost colorimetric assays that can be applied to the detection of small molecules, DNA, proteins and pollutants. The main aim of the study was the improving of enzyme immunoassay for detection and quantification of the target molecules using silver nanoparticles. For this purpose we developed method for synthesis of silver nanoparticles with hyaluronic acid and studied possibility of use these nanoparticles in direct determination of target molecules concentration (in particular proteins) and for improving of enzyme immunoassay. As model we used conventional enzyme immunoassays for determination of progesterone and estradiol concentration. We obtained the possibility to produce silver nanoparticles with hyaluronan homogeneous in size between 10 and 12 nm, soluble and stable in water during long term of storage using modified procedure of silver nanoparticles synthesis. New method allows to obtain silver nanoparticles with strong optical properties at the higher concentrations -60-90 μg/ml with the peak of absorbance at the wavelength 400 nm. Therefore surface plasmon resonance of silver nanoparticles with hyaluronan and ultraviolet-visible spectroscopy provide an opportunity for rapid determination of target molecules concentration (especial protein). We used silver nanoparticles as enzyme carriers and signal enhancers. Our preliminary data show that silver nanoparticles increased absorbance of samples that allows improving upper limit of determination of estradiol and progesterone concentration.

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Colorimetric Immunoassay for Detection of Tumor Markers

Cited by 60 publications

References 80 publications

Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Label-free immunodetection of the cancer biomarker CA125 using high-Δn liquid crystals

Improving of enzyme immunoassay for detection and quantification of the target molecules using silver nanoparticles

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