Colorimetric Determination of Ribose, Deoxyribose, and Nucleic Acid with Anthrone

Gary, N. D.; Klausmeier, R. E.

doi:10.1021/ac60096a028

Cited by 15 publications

(6 citation statements)

References 2 publications

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“…The Folin–Ciocalteu method was used to quantify total phenolic compounds in POMx. Stock solutions of gallic acid (1 mg/mL) were used to generate four-point (0.005, 0.015, 0.03, and 0.05 mg) standard curves for the estimation of total polyphenols.…”

Section: Experimental Methodsmentioning

confidence: 99%

Safety and Antioxidant Activity of a Pomegranate Ellagitannin-Enriched Polyphenol Dietary Supplement in Overweight Individuals with Increased Waist Size

Heber

Seeram²,

Wyatt³

et al. 2007

J. Agric. Food Chem.

170

121

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The consumption of pomegranate juice (PJ), a rich source of antioxidant polyphenols, has grown tremendously due to its reported health benefits. Pomegranate extracts, which incorporate the major antioxidants found in pomegranates, namely, ellagitannins, have been developed as botanical dietary supplements to provide an alternative convenient form for consuming the bioactive polyphenols found in PJ. Despite the commercial availability of pomegranate extract dietary supplements, there have been no studies evaluating their safety in human subjects. A pomegranate ellagitannin-enriched polyphenol extract (POMx) was prepared for dietary supplement use and evaluated in two pilot clinical studies. Study 1 was designed for safety assessment in 64 overweight individuals with increased waist size. The subjects consumed either one or two POMx capsules per day providing 710 mg (435 mg of gallic acid equivalents, GAEs) or 1420 mg (870 mg of GAEs) of extracts, respectively, and placebo (0 mg of GAEs). Safety laboratory determinations, including complete blood count (CBC), chemistry, and urinalysis, were made at each of three visits. Study 2 was designed for antioxidant activity assessment in 22 overweight subjects by administration of two POMx capsules per day providing 1000 mg (610 mg of GAEs) of extract versus baseline measurements. Measurement of antioxidant activity as evidenced by thiobarbituric acid reactive substances (TBARS) in plasma were measured before and after POMx supplementation. There was evidence of antioxidant activity through a significant reduction in TBARS linked with cardiovascular disease risk. There were no serious adverse events in any subject studied at either site. These studies demonstrate the safety of a pomegranate ellagitannin-enriched polyphenol dietary supplement in humans and provide evidence of antioxidant activity in humans.

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Section: Experimental Methodsmentioning

confidence: 99%

Safety and Antioxidant Activity of a Pomegranate Ellagitannin-Enriched Polyphenol Dietary Supplement in Overweight Individuals with Increased Waist Size

Heber

Seeram²,

Wyatt³

et al. 2007

J. Agric. Food Chem.

170

121

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“…POMx powder, PPS1 and PPS2 were analyzed for total carbohydrate content using the anthrone method of Gary and Klausmeier. 14 Briefly, analytes (0.1 g) were solubilized in 15 mL water and loaded onto a C18 reverse-phase cartridge (Sep-Pak Vac 20 cc, 5g, Milford, MA) previously conditioned with methanol and equilibrated with water. After washing the column with 100 mL water (1.0 mL L −1 TFA), the eluate was dried by rotary evaporation (<30 • C) under vacuum.…”

Section: Total Carbohydrate Determinationmentioning

confidence: 99%

Development of a novel pomegranate standard and new method for the quantitative measurement of pomegranate polyphenols

et al. 2008

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BACKGROUND: The Folin-Ciocalteu method is routinely used to calculate total polyphenol concentrations (TPP) in food products. However, the use of inappropriate standards such as gallic acid (GA) may give inaccurate estimates of TPP content. A method for the production of a pomegranate (Punica granatum L. 'Wonderful') standard was developed and validated to avoid this problem. The PPS was produced by solid phase (C18) isolation of polyphenols from POMx powder (a nutritional supplement) and used for TPP analysis.

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“…Analytical methods. Total carbohydrate in growth filtrates was determined by a modification of the anthrone method of Gary and Klausmeier (1954), in which the reactant tubes of the samples as well as the glucose standard were heated in a boiling-water bath for 10 min and read at 620 m,u in a model 14 Coleman Universal spectrophotometer. Lactate was determined by the method of Barker and Summerson as outlined by Umbreit, Burris, and Stauffer (1949), pyruvate by the method of Friedemann and Haugen (1943), and acetoin by the method of WVesterfield (1945).…”

Section: Methodsmentioning

confidence: 99%

EFFECT OF NUTRITION ON THE RESPIRATORY VIRULENCE OF LISTERIA MONOCYTOGENES

Friedman

Kautter

1962

J Bacteriol

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Friedman, Mischa E. (U. S. Army Chemical Corps, Frederick, Md.) and Donald A. Kautter , Effect of nutrition on the respiratory virulence of Listeria monocytogenes . J. Bacteriol. 83: 456–462. 1962.—The respiratory virulence for the mouse and guinea pig of two strains of Listeria monocytogenes grown in a rich nonsynthetic medium may be increased four to ninefold by decreasing the glucose concentration from 1.0 to 0.6% or by including 0.1% l -cysteine in the medium. This effect, not observed in intraperitoneal challenge of mice, is not due to selection of a more virulent population. Cultures demonstrating lowered virulence at 16 hr increase in virulence if incubated beyond 50 hr. The presence of 1.0% fructose or cellobiose results in the same growth and virulence characteristics as when glucose is used; however, cultures in media with 1.0% maltose are of decreased cell yield but increased virulence. Strain A4413 grown in media of low glucose concentration or in the presence of cysteine has greater oxidative activity than when grown in a medium of high glucose content. Other strains of lesser respiratory virulence, when grown in a low concentration of glucose, contain glucose dehydrogenase systems equal in activity with that of strain A4413.

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Colorimetric Determination of Ribose, Deoxyribose, and Nucleic Acid with Anthrone

Cited by 15 publications

References 2 publications

Safety and Antioxidant Activity of a Pomegranate Ellagitannin-Enriched Polyphenol Dietary Supplement in Overweight Individuals with Increased Waist Size

Safety and Antioxidant Activity of a Pomegranate Ellagitannin-Enriched Polyphenol Dietary Supplement in Overweight Individuals with Increased Waist Size

Development of a novel pomegranate standard and new method for the quantitative measurement of pomegranate polyphenols

EFFECT OF NUTRITION ON THE RESPIRATORY VIRULENCE OF LISTERIA MONOCYTOGENES

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