Colorimetric Determination of Polyamidoamine Dendrimers and their Derivates using a Simple and Rapid Ninhydrin Assay

Xu, Zhenhua; Xu, Tongwen; Cheng, Yiyun; Ma, Minglu; Xu, Peng; Qu, Haiou; Wen, Longping

doi:10.1080/00032710701484350

Cited by 9 publications

(8 citation statements)

References 10 publications

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“…To quantify the amount of primary amines chemically available on the surface of the PSilQ NPs after functionalization with a PEI polymer, the ninhydrin-based Kaiser’s test was performed . The amounts of chemically accessible primary amines obtained for PEG-PEI-PSilQ(cur) and PEI-PSilQ(cur) NPs were 328 ± 10 and 536 ± 45 nmol per mg of nanoparticles ( n = 3), respectively (Table S1).…”

Section: Resultsmentioning

confidence: 99%

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Multimodal Polysilsesquioxane Nanoparticles for Combinatorial Therapy and Gene Delivery in Triple-Negative Breast Cancer

Juneja

Lyles

Vadarevu

et al. 2019

ACS Appl. Mater. Interfaces

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Multifunctional hybrid nanoparticles are being developed to carry a wide variety of therapeutic and imaging agents for multiple biomedical applications. Polysilsesquioxane (PSilQ) nanoparticles are a promising hybrid platform with numerous advantages to be used as a delivery system. In this report, we demonstrate the ability of a stimuli-responsive PSilQ-based platform to transport and deliver simultaneously protoporphyrin IX, curcumin, and RNA interference inducers inside human cells. This multimodal delivery system shows a synergistic performance for the combined phototherapy and chemotherapy of triple-negative breast cancer and can be used for efficient transfection of therapeutic nucleic acids. The current work represents the first report of using the PSilQ platform for the combined phototherapy and chemotherapy and gene delivery.

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Section: Resultsmentioning

confidence: 99%

“…The PEG-PEI-PSilQ(cur) NPs were redispersed and stored in ethanol at 4 °C. The amount of amine groups chemically available on the surface of PEG-PEI-PSilQ(cur) NPs was indirectly quantified using the Kaiser’s test …”

Section: Methodsmentioning

confidence: 99%

Multimodal Polysilsesquioxane Nanoparticles for Combinatorial Therapy and Gene Delivery in Triple-Negative Breast Cancer

Juneja

Lyles

Vadarevu

et al. 2019

ACS Appl. Mater. Interfaces

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“…The changes on the ζ‐potential values through the different functionalization steps for the synthesis of the PEG‐MSN confirm the successful modifications of the material (Table S2, Supporting Information). The electrostatically driven binding between the negatively charged phosphate backbone of nucleic acids and positive amine groups on the PEG‐MSN is evaluated at different N/P ratios (N = number of moles of free amines on MSN as determined by a ninhydrin assay; P = number of moles of phosphates) by electrophoretic mobility shift assay (Figure S6, Supporting Information). The optimal N/P ratio is determined to be 10 and then used for all subsequent studies.…”

Section: Resultsmentioning

confidence: 99%

RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition

Rackley

Stewart

Salotti

et al. 2018

Adv Funct Materials

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RNA is a versatile biomaterial that can be used to engineer nanoassemblies for personalized treatment of various diseases. Despite promising advancements, the design of RNA nanoassemblies with minimal recognition by the immune system remains a major challenge. Here, an approach is reported to engineer RNA fibrous structures to operate as a customizable platform for efficient coordination of siRNAs and for maintaining low immunostimulation. Functional RNA fibers are studied in silico and their formation is confirmed by various experimental techniques and visualized by atomic force microscopy (AFM). It is demonstrated that the RNA fibers offer multiple advantages among which are: i) programmability and modular design that allow for simultaneous controlled delivery of multiple siRNAs and fluorophores, ii) reduced immunostimulation when compared to other programmable RNA nanoassemblies, and iii) simple production protocol for endotoxin-free fibers with the option of their cotranscriptional assembly. Furthermore, it is shown that functional RNA fibers can be efficiently delivered with various organic and inorganic carriers while retaining their structural integrity in cells. Specific gene silencing triggered by RNA fibers is assessed in human breast cancer and melanoma cell lines, with the confirmed ability of functional fibers to selectively target single nucleotide mutations.

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“…The stock suspension of well-defined concentration was purified by dialysis using a Slide-A-Lyzer dialysis cassette (Thermo Fisher Scientific, Waltham, MA, USA) to remove low-molecular-weight impurities. Using ultraviolet-visible spectrophotometry, we determined the exact concentration of G6 PAMAM dendrimers after purification using the ninhydrin assay 43. This procedure is based on the interaction of the primary amino group of PAMAM dendrimers with ninhydrin (Sigma-Aldrich) to form a blue product.…”

Section: Methodsmentioning

confidence: 99%

<p>Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo</p>

Dąbkowska

Rogińska

Kłos

et al. 2019

IJN

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Background: NT4 has been regarded as a promising therapeutic protein for treatment of damaged retinal pigment epithelium cells. Purpose: Here, we studied physicochemical parameters of an NT4–polyamidoamine (PAMAM) electrostatic complex, which can provide a sustained concentration of protein in intraocular space over an extended period after delivery. Adsorption/desorption of NT4 molecules to/from positively charged PAMAM dendrimers were precisely determined to control the concentration of bounded/unbounded protein molecules, diffusion coefficient, and size of a protein-laden dendrimer structure. We determined kinetics of NT4 desorption in PBS, vitreous, and damaged retina. Methods: Initially, adsorption of NT4 molecules on PAMAM dendrimers was studied in PBS using dynamic light scattering, electrophoresis, solution depletion, ELISA, and atomic force microscopy. This allowed us precisely to determine desorption of NT4 from nanoparticles under in situ conditions. The maximum coverage of irreversibly adsorbed NT4 determined by ELISA allowed us to devise a robust procedure for preparing stable and well-controlled coverage of NT4 on PAMAM nanoparticles. Thereafter, we studied diffusion of nanospheres containing NT4 molecules by injecting them into vitreous cavities of mice exposed to intravenous injections of sodium iodate and evaluated their intraocular desorption kinetics from drug carriers in vivo. Results: Our measurements revealed NT4–dendrimer nanoparticles can be used for continuous neurotrophic factor delivery, enhancing its distribution into mouse vitreous, as well as damaged retina over 28 days of postinjury observation. Conclusion: Understanding of polyvalent neurotrophin interactions with dendrimer nanoparticles might be useful to obtain well-ordered protein layers, targeting future development of drug-delivery systems, especially for neuroprotection of damaged retinal neurons.

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Colorimetric Determination of Polyamidoamine Dendrimers and their Derivates using a Simple and Rapid Ninhydrin Assay

Cited by 9 publications

References 10 publications

Multimodal Polysilsesquioxane Nanoparticles for Combinatorial Therapy and Gene Delivery in Triple-Negative Breast Cancer

Multimodal Polysilsesquioxane Nanoparticles for Combinatorial Therapy and Gene Delivery in Triple-Negative Breast Cancer

RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition

<p>Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo</p>

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