Colorimetric Determination of Indene

Skoog, Douglas A.; Dubois, H

doi:10.1021/ac60036a028

Cited by 5 publications

(2 citation statements)

References 3 publications

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“…The conditional binding constants were determined as described previously, [35] taking into account the different pKa values for the EDTA ligand [35,40–42] …”

Section: Methodsmentioning

confidence: 99%

Bi(III) Binding Stoichiometry and Domain‐Specificity Differences Between Apo and Zn(II)‐bound Human Metallothionein 1a

Korkola,

Ostertag,

Toswell

et al. 2024

Chemistry A European J

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Bismuth is a xenobiotic metal with a high affinity to sulfur that is used in a variety of therapeutic applications. Bi(III) induces the cysteine‐rich metallothionein (MT), a protein known to form two‐domain cluster structures with certain metals such as Zn(II), Cd(II), or Cu(I). The binding of Bi(III) to MTs has been previously studied, but there are conflicting reports on the stoichiometry and binding pathway, which appear to be highly dependent on pH and initial metal‐loading status of the MT. Additionally, domain specificity has not been thoroughly investigated. In this paper, ESI‐MS was used to determine the binding constants of [Bi(EDTA)]‐ binding to apo‐MT1a and its individual αMT fragment. The results were compared to previous experiments using βMT1a and βαMT3. Domain specificity was investigated using proteolysis methods and the initial cooperatively formed Bi2MT was found to bind to cysteines that spanned across the traditional metal binding domain regions. Titrations of [Bi(EDTA)]‐ into Zn7MT were performed and were found to result in a maximum stoichiometry of Bi7MT, contrasting the Bi6MT formed when [Bi(EDTA)]‐ was added to apo‐MT. These results show that the initial structure of the apo‐MT determines the stoichiometry of new incoming metals and explains the previously observed differences in stoichiometry.

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“…The conditional binding constants were determined as described previously, [35] taking into account the different pKa values for the EDTA ligand [35,40–42] …”

Section: Methodsmentioning

confidence: 99%

Bi(III) Binding Stoichiometry and Domain‐Specificity Differences Between Apo and Zn(II)‐bound Human Metallothionein 1a

Korkola,

Ostertag,

Toswell

et al. 2024

Chemistry A European J

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show abstract

“…As examples of new7 methods for organic constituents the following were selected: for Chloromycetin, reduce the aryl nitro group, diazotize, and couple to give a red color (16); for serum lipase, couple the 2-naphthol, produced on hydrolysis, with diorthoanisidine (170); for tryptophan, condense wdth p-dimethylaminobenzaldehyde and develop the color with sodium nitrite (188); Aldrin reacts w7ith phenylazide to give dihydro triazole, which is then coupled with diazotized 2,4-dinitroaniline (S3); indene reacts with benzaldehyde in presence of alkali (183); lactic acid reacts with concentrated sulfuric acid and veratrole in absolute ethyl alcohol to give a red color (132); reducing sugars react with triphenyltetrazolium chloride to yield formazan (131); resorcinol iodinates in the presence of catechol to give a precipitate which dissolves in acetone to give a colored system (212); ethanolic extracts of nordihydroguaiaretic acid react with ammonium molybdate to give an orange color (47); triethylene glycol in sulfuric acid reacts with 1-naphthol to give a yellow color (99).…”

Section: Chemistrymentioning

confidence: 99%