Colorimetric determination of 8-hydroxy–2′-deoxyguanosine using label-free aptamer and unmodified gold nanoparticles

Wang, Jiacheng; Wang, Yongsheng; Rang, Wei-Qing; Xue, Jiaying; Zhou, Bin; Liu, Lu; Qian, Qiu-Mei; Wang, Yong-Song; Yin, Ji-Cheng

doi:10.1007/s00604-014-1173-1

Cited by 25 publications

(21 citation statements)

References 33 publications

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“…The method worked in the diluted human serum (1:10 ratio) samples spiked with various concentrations of o 8 dGuo. This method is not as fast as the previously reported method based on aptamer and unmodified AuNPs [ 38 ] because of several incubation procedures, but the LOD of this method is lower by nearly three orders of magnitude than that of earlier methods ( Figure 9 ).…”

Section: Analytical Detection and Quantitation Methodsmentioning

confidence: 69%

“…Beyond the information on the quantity, concentration or frequency of oxidative lesions, in some cases, additional pieces of information can hint at temperature-dependence, kinetic and thermodynamic parameters [29], processes preceding oxidation [41] or eventual cross-link formation in short ON tracts [44]. The transformation of G can take place in different sequence contexts, including GQs, which have also been utilized in some analytical methods [34,35,[38][39][40].…”

Section: Summary and Comparison Of The Efficacy Of Analytical Methodsmentioning

confidence: 99%

“…A similarly sensitive and specific colorimetric method has been elaborated earlier by Wang et al [ 38 ] for urine sample analysis. The method consists of three steps: (1) the aptamer was adsorbed on the surface of AuNPs which enhances their stability, (2) upon addition of o 8 dGuo, the conformation of the aptamer changes to form a GQ structure and (3) as a consequence, it loses the ability to protect the nanoparticles and causes a color change from red to blue.…”

Section: Analytical Detection and Quantitation Methodsmentioning

confidence: 99%

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Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

Ferenc

Váradi

Kupihár

et al. 2020

IJMS

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DNA damage plays a decisive role in epigenetic effects. The detection and analysis of DNA damages, like the most common change of guanine (G) to 8-oxo-7,8-dihydroguanine (OG), is a key factor in cancer research. It is especially true for G quadruplex structure (GQ), which is one of the best-known examples of a non-canonical DNA arrangement. In the present work, we provided an overview on analytical methods in connection with the detection of OG in oligonucleotides with GQ-forming capacity. Focusing on the last five years, novel electrochemical tools, like dedicated electrodes, were overviewed, as well as different optical methods (fluorometric assays, resonance light scattering or UV radiation) along with hyphenated detection and structural analysis methods (CD, NMR, melting temperature analysis and nanopore detection) were also applied for OG detection. Additionally, GQ-related computational simulations were also summarized. All these results emphasize that OG detection and the analysis of the effect of its presence in higher ordered structures like GQ is still a state-of-the-art research line with continuously increasing interest.

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Section: Analytical Detection and Quantitation Methodsmentioning

confidence: 69%

Section: Summary and Comparison Of The Efficacy Of Analytical Methodsmentioning

confidence: 99%

Section: Analytical Detection and Quantitation Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

Ferenc

Váradi

Kupihár

et al. 2020

IJMS

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“…The 66-nt 8-oxo-dG aptamer was established by the SELEX method and reported in the literature. 36 , 44 , 50 Based on the parent 66-nt aptamer sequence, we previously designed and reported a shortened aptamer sequence of 38-nt. 33 Both aptamers have been applied in the development of 66-nt and 38-nt aptasensors.…”

Section: Discussionmentioning

confidence: 99%

Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine

et al. 2020

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8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG), an oxidized form of guanosine residues, is a critical biomarker for various cancers. Herein, a sensitive citrate-capped gold nanoparticle-based aptasensor device has been developed for the detection of 8-oxo-dG in urine. We previously designed a 38-nt anti-8-oxo-dG-aptamer by a computer simulation and the experimental validation has been performed in the present work. The analytical performance of the 38-nt aptamer from the in silico design was compared with the parent 66-nt aptamer. This assay is based on the principle of salt-induced aggregation of citrate-capped gold nanoparticles. Based on this sensing mechanism, the difference between the absorbance in the presence and absence of 8-oxo-dG at λ = 525 nm (ΔA525) increased linearly as a function of 8-oxo-dG concentrations in the ranges of 10–100 and 15–100 nM for 38-nt and 66-nt aptasensors, respectively. This method can provide detection limits of 6.4 nM for 8-oxo-dG in the 38-nt aptasensor and 13.2 nM in the 66-nt aptasensor. Similar to the 66-nt aptamer, the shortened aptamer, 38-nt long, can provide high sensitivity and selectivity with rapid detection time. In addition, using the 38-nt aptamer as a recognition component in the developed portable low-cost device showed high sensitivity in the detection range of 15–100 nM with a detection limit of 12.9 nM, which is much lower than the threshold value (280 nM) for normal human urine. This easy-to-use device could effectively and economically be utilized for monitoring 8-oxo-dG in real urine samples and potentially serve as a prototype for a commercial device.

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“…The most commonly used analytical techniques for the detection of 8-OHdG in urine are high performance liquid chromatography-electrochemical detection (HPLC-ECD) [244], HPLC tandem mass spectrometry (HPLC MS/MS) [245], capillary electrophoresis with electrochemical detection (CE-ECD) [242,246], electrochemical methods including cyclic voltammetry (CV) [247][248][249], differential pulse voltammetry [250] and linear sweep voltammetry (LSV) [251]. Ultraviolet spectroscopy [252,253],…”

Section: Discussionmentioning

confidence: 99%

Point of care diagnostics for carbonyl/oxidative stress biomarkers

Gopal¹

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Prof. Bernhard Boehm for their advices, knowledge sharing and constructive comments to understand the subject from the biological/clinical perspective.I sincerely thank my mentor, Dr. Alagappan Palaniappan, for his thoughtful and patient supervision, encouragement and useful suggestions for my project and the report. I would also like to thank Asst. Prof. Hakan Yildiz and Dr. Deepa Rajwar, for their help on polythiophene synthesis, characterizations, and insightful discussions on the project.

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Colorimetric determination of 8-hydroxy–2′-deoxyguanosine using label-free aptamer and unmodified gold nanoparticles

Cited by 25 publications

References 33 publications

Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine

Point of care diagnostics for carbonyl/oxidative stress biomarkers

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