Colorimetric Determination of 2-Nitro-1,1-bis(p-Chlorophenyl) Alkanes

Jones, L. R.; Riddick, John A.

doi:10.1021/ac60050a033

Cited by 13 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various papers have been published (Clifford, 1947 ;Schechter, Pogorelskin & Haller, 1947 ;Fairing & Warrington, 1950;Jones & Riddick, 1951, 1952Krzeminski & Landmann, 1963) describing the isolation of minute amounts of pesticides from biological specimens by means that are usually complex and tedious. Extensive cleanup is required, and is accomplished for example by hexane : aceto-nitrile partitioning (Jones & Riddick, 1952;McKinley & Savary, 1962), paper chromatography (Mitchell, 1957;Muller, Ernst & Schock, 1957;MacRae & McKinley, 1961), thin layer chromatography (Walker and Beroza, 1963), silicic acid (Moats, 1962) and florisil column chromatography (Moddes, 1961 ;Moats, 1963).…”

mentioning

confidence: 99%

Simplified gas chromatographic analysis of pesticides from blood

Jain

Fontan

Kirk

1965

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

A rapid method for the extraction and identification of 23 pesticides present in blood is described. The pesticides are extracted from blood with a mixture of acetone and ethyl ether in equal volumes. The extracts are evaporated to dryness, the residue dissolved in a known quantity of hexane and an aliquot injected directly into the gas chromatograph equipped with an electron capture detector. No purification of the acetone-ether extract is required. A glass column packed with 5% SE 52 on hexamethyldisilizane-treated chromosorb W 60180 is used at a temperature of 190". The method was successfully applied to blood from laboratory animals to which acute doses of six of the most common pesticides were given orally. The quantity of pesticides found is reported.YSTEMATIC schemes for the isolation and identification of toxic S substances are largely confined to conventional poisons. With the advent of many new compounds having toxic properties, the deficiencies of the classical procedures have been made more obvious. This is especially true of the group of pesticides which includes chlorinated hydrocarbons and organophosphorus compounds. Symptoms associated with these have been treated as chronic rather than acute effects, and analysis has largely been directed towards the control of pesticide residues on foodstuffs.Acute poisoning from these materials is increasing and the toxicologist needs to be able to identify rapidly and with certainty the presence in physiological materials of acutely toxic quantities of such materials. The quantities of pesticides in such cases are so small, and the conventional tests so insensitive and non-specific, that these poisons may readily be overlooked. It is therefore essential to make available simple and rapid methods of extraction to be used with detection methods to ensure that toxic material is not overlooked. Also the procedure should not require extensive clean up of the extracts before it can be applied.Various papers have been published (

show abstract

mentioning

confidence: 99%

Simplified gas chromatographic analysis of pesticides from blood

Jain

Fontan

Kirk

1965

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

show abstract

“…A ten-minute reaction time a t 100' was chosen to insure complete hydralysis. Color Formation.-The absorption spectra, the effect of water on the colored complex, the effect of acidity on the intensity of color, the reaction time and temperature, and the validity of Beer's law were compared and found to coincide with the results obtained and reported for the determination of 2-nitro-l,l-bis(~-chlorophenyl)alkanes (1).…”

mentioning

confidence: 56%

Colorimetric Determination of 2-Nitro-2-bromo-1-methoxy-1-phenylpropane

Jones

Riddick

1954

Journal of the American Pharmaceutical Association (Scientific

View full text Add to dashboard Cite

“…These residual biological materials can be eliminated if the alcoholic sample, used in the color development, is divided into two parts of 5 ml. each; one is treated in the regular manner (9) , and the order of adding sodium hydroxide and ferric chloride reagents is reversed on the other. When this procedure is followed the color does not develop in the latter solution.…”

Section: Discussionmentioning

confidence: 99%

“…The methods for analyzing the insecticides per se generally are sufficiently sensitive and accurate for microgram quantities. There are specific methods for isolating microgram quantities of some insecticides from biological samples (5-7), (9,10), but they usually are long and complicated. A general method that is simple and rapid for isolating insecticides from fats, waxes, and other biological substances should find wide application.…”

Section: Literature Citedmentioning

confidence: 99%