Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity

Wen, Junlin; Zhou, Shungui; Chen, Junhua

doi:10.1038/srep05191

Cited by 37 publications

(14 citation statements)

References 36 publications

(36 reference statements)

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“…The SEM images provided visual evidence showing the successful binding between the bacteria and the particles. The incubation time and binding efficacy here were consistent with those in previous studies based on the bead-based immunoassay [ 14 , 21 , 29 ].…”

Section: Resultssupporting

confidence: 90%

Rapid Bead-Based Antimicrobial Susceptibility Testing by Optical Diffusometry

2016

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This study combined optical diffusometry and bead-based immunoassays to develop a novel technique for quantifying the growth of specific microorganisms and achieving rapid AST. Diffusivity rises when live bacteria attach to particles, resulting in additional energy from motile microorganisms. However, when UV-sterilized (dead) bacteria attach to particles, diffusivity declines. The experimental data are consistent with the theoretical model predicted according to the equivalent volume diameter. Using this diffusometric platform, the susceptibility of Pseudomonas aeruginosa to the antibiotic gentamicin was tested. The result suggests that the proliferation of bacteria is effectively controlled by gentamicin. This study demonstrated a sensitive (one bacterium on single particles) and time-saving (within 2 h) platform with a small sample volume (~0.5 μL) and a low initial bacteria count (50 CFU per droplet ~ 105 CFU/mL) for quantifying the growth of microorganisms depending on Brownian motion. The technique can be applied further to other bacterial strains and increase the success of treatments against infectious diseases in the near future.

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Section: Resultssupporting

confidence: 90%

Rapid Bead-Based Antimicrobial Susceptibility Testing by Optical Diffusometry

2016

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“…On this basis, we further expanded the technique to AST for co-cultured bacteria by drawing on the features of bead-based immunoassays. The incubation time and binding efficacy for particle–bacterium binding in the present study were consistent with those of the bead-based immunoassay in previous studies 16 26 31 . Although non-specific bindings remain unavoidable without a special surface modification 32 , this result proved that the simultaneous detection of multipathogens is achieved through immunoassays.…”

Section: Discussionsupporting

confidence: 91%

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

Chung

Wang

Chuang

2017

Sci Rep

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Successful treatments against bacterial infections depend on antimicrobial susceptibility testing (AST). However, conventional AST requires more than 24 h to obtain an outcome, thereby contributing to high patient mortality. An antibiotic therapy based on experiences is therefore necessary for saving lives and escalating the emergence of multidrug-resistant pathogens. Accordingly, a fast and effective drug screen is necessary for the appropriate administration of antibiotics. The mixed pathogenic nature of infectious diseases emphasizes the need to develop an assay system for polymicrobial infections. On this basis, we present a novel technique for simultaneous and quantitative monitoring of co-cultured microorganisms by coupling optical diffusometry with bead-based immunoassays. This simple integration simultaneously achieves a rapid AST analysis for two pathogens. Triple color particles were simultaneously recorded and subsequently analyzed by functionalizing different fluorescent color particles with dissimilar pathogen-specific antibodies. Results suggested that the effect of the antibiotic, gentamicin, on co-cultured Pseudomonas aeruginosa and Staphylococcus aureus was effectively distinguished by the proposed technique. This study revealed a multiplexed and time-saving (within 2 h) platform with a small sample volume (~0.5 μL) and a low initial bacterial count (50 CFU per droplet, ~105 CFU/mL) for continuously monitoring the growth of co-cultured microorganisms. This technique provides insights into timely therapies against polymicrobial diseases in the near future.

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“…Many oxygenase and reductase reactions may be involved in metal ion facilitation in bacteria [ 58 ]. The ability of S. oneidensis to reduce oxidized metals or nitrate effectively has been identified as an important intrinsic activity of Shewanella species [ 59 – 62 ]. These results all indicate that S. algae is capable of adapting to environmental changes.…”

Section: Discussionmentioning

confidence: 99%

The Pathogenicity ofShewanella algaeand Ability to Tolerate a Wide Range of Temperatures and Salinities

Tseng

Liu

Lee

et al. 2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Shewanella algae is a rod-shaped Gram-negative marine bacterium frequently found in nonhuman sources such as aquatic ecosystems and has been shown to be the pathogenic agent in various clinical cases due to the ingestion of raw seafood. The results of this study showed that S. algae was present in approximately one in four samples, including water and shellfish samples. Positive reactions (API systems) in S. algae strains were seen for gelatinase (gelatin); however, negative reactions were found for indole production (tryptophan). S. algae is adapted to a wide range of temperatures (4°C, 25°C, 37°C, and 42°C) and salinity. Temperature is a key parameter in the pathogenicity of S. algae as it appears to induce hemolysis at 25°C and 37°C. S. algae exhibits pathogenic characteristics at widely varying temperatures, which suggests that it may have the ability to adapt to climate change.

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Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity

Cited by 37 publications

References 36 publications

Rapid Bead-Based Antimicrobial Susceptibility Testing by Optical Diffusometry

Rapid Bead-Based Antimicrobial Susceptibility Testing by Optical Diffusometry

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

The Pathogenicity ofShewanella algaeand Ability to Tolerate a Wide Range of Temperatures and Salinities

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