Colorimetric Detection of <i>Escherichia coli</i> by Polydiacetylene Vesicles Functionalized with Glycolipid

Ma, Zhanfang; Li, Jinru; Liu, Minghua; Cao, Jie; Zou, Zhiying; Tu, Jing; Jiang, Long

doi:10.1021/ja982663a

Cited by 159 publications

(118 citation statements)

References 12 publications

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“…Previous studies have employed chemically derivatized PDA for bacterial sensing; however, those systems relied mostly on the covalently displaying specific recognition units on the PDA surface, producing signals only through actual binding of bacterial proteins to the PDA matrix. Such assemblies require chemical modifications of the PDA units, and their detection levels are determined by the number of bacterial particles attaching to the PDA-displayed receptors (15,23). A previous study employing PDA for bacterial sensing exhibited a detection limit of 10 8 bacterial particles (16).…”

Section: Resultsmentioning

confidence: 99%

Rapid Chromatic Detection of Bacteria by Use of a New Biomimetic Polymer Sensor

Silbert¹,

Shlush²,

Israel

et al. 2006

Appl Environ Microbiol

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We present a new platform for visual and spectroscopic detection of bacteria. The detection scheme is based on the interaction of membrane-active compounds secreted by bacteria with agar-embedded nanoparticles comprising phospholipids and the chromatic polymer polydiacetylene (PDA). We demonstrate that PDA undergoes dramatic visible blue-to-red transformations together with an intense fluorescence emission that are induced by molecules released by multiplying bacteria. The chromatic transitions are easily identified by the naked eye and can also be recorded by conventional high-throughput screening instruments. Furthermore, the color and fluorescence changes generally occur in shorter times than the visual appearance of bacterial colonies on the agar. The chromatic technology is generic and simple, does not require identification a priori of specific bacterial recognition elements, and can be applied for detection of both gram-negative and grampositive bacteria. We demonstrate applications of the new platform for reporting on bacterial contaminations in foods and for screening for bacterial antibiotic resistance.The emerging global risks of bioterrorism, the recurring incidents of bacterial food contaminations, and the need to monitor sterile environments in health care applications and other industries have pushed the development of methods for detection of pathogens to the forefront of technological and scientific research. Numerous technologies for reporting on bacterial presence have been developed (3,5,9,25,26). There are, however, limitations to existing bacterial detection techniques as rapid and generic approaches. Specifically, many bioanalytical techniques employed for pathogen detection (such as culture-based methods) provide results after relatively long time spans (several hours to days) (10). Other currently employed technologies often involve complex detection mechanisms that require specialized instrumentation, application by trained personnel, and the need for active operation (addition of reagents, initiation of chemical reactions, etc.), which overall do not lend their use in settings other than laboratory environments (3, 6). Furthermore, a prerequisite for many detection methods is the detailed understanding of the biochemical and structural properties of the bacterial species sought, limiting applications in the case of unknown pathogens or variants (9, 31).Various membrane-active compounds are released by bacteria to their environments (2, 30), a process that often has an essential functional role as a means for overcoming host defense mechanisms, allowing colony proliferation, and facilitating bacterial communication (17). Membrane-active peptides and toxins, in particular, are produced by bacteria, for example, pneumolysins secreted by streptococci (27) and ␣-toxin, which is the major cytolysin emitted by Staphylococcus aureus (1). Secretion of pore-forming exotoxins by bacteria is abundant, and endotoxins, such as lipopolysaccharides, which are often released by gram-negative bacteria, ...

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Section: Resultsmentioning

confidence: 99%

Rapid Chromatic Detection of Bacteria by Use of a New Biomimetic Polymer Sensor

Silbert¹,

Shlush²,

Israel

et al. 2006

Appl Environ Microbiol

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“…[2][3][4][5][6][7][8][9][10][11] It is well known that the spacially aligned monomeric diacetylene moieties undergo photo polymerization process via a 1,4-addition mechanism and form conjugated chains that give the molecule a significant color change. 12 Due to this unique color change, efforts have been devoted to develop efficient sensor systems based on this polyacetylenes.…”

Section: Methodsmentioning

confidence: 99%

Preparation of Carbohydrate Derivatives with PCDA Tails: Applicationfor Cell Surface Recognition

2009

Bulletin of the Korean Chemical Society

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“…9,10 Because the polymerization does not require a chemical initiator or catalyst, this process has the added advantage of avoiding the need for a purification step. The conjugated groups of PDA undergo further conformational changes in response to external stimuli such as heat, [11][12][13] organic solvents, 14,15 mechanical stress, [16][17][18] or biomolecule addition, [19][20][21][22][23][24] resulting in distinctive chromic changes that can be exploited for the development of colorimetric chemosensors or biomolecular sensors. Herein, we constructed a PEGylated PDA liposomal nanovesicle and characterized its unique properties along with the inclusion complex formation by PEGinduced interaction with α-cyclodextrin.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of PEGylated Polydiacetylene Liposome and its Inclusion Complex Formation with α-Cyclodextrin

Choi¹,

Choi²

2013

Bulletin of the Korean Chemical Society

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Diacetylene lipid monomers possess the capability to self-assemble into vesicles via polymerization under ultraviolet irradiation, resulting in the formation of polydiacetylene (PDA) liposomes. Exposure of the polymerized vesicles to external stimuli is known to induce a unique blue-to-red color transition. The cyclic oligosaccharide α-cyclodextrin known for its use in many applications, such as drug delivery, purification, and stimulus sensing, is able to form an inclusion complex with poly(ethylene glycol) (PEG) in aqueous solution. In this study, we prepared polymeric liposomes with PEG (PEG-PDA) with the aim of improving the stability of the vesicles and colorimetric response toward α-cyclodextrin. We demonstrated that PEG-PDA liposome displays unique characteristics compared with native PDA liposome and it also shows apparent chromic properties of the inclusion complex formation with α-cyclodextrin.

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Colorimetric Detection of Escherichia coli by Polydiacetylene Vesicles Functionalized with Glycolipid

Cited by 159 publications

References 12 publications

Rapid Chromatic Detection of Bacteria by Use of a New Biomimetic Polymer Sensor

Rapid Chromatic Detection of Bacteria by Use of a New Biomimetic Polymer Sensor

Preparation of Carbohydrate Derivatives with PCDA Tails: Applicationfor Cell Surface Recognition

Characteristics of PEGylated Polydiacetylene Liposome and its Inclusion Complex Formation with α-Cyclodextrin

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