Colorimetric detection of Helicobacter pylori DNA using isothermal helicase-dependent amplification and gold nanoparticle probes

Gill, Pooria; Alvandi, Amirhooshang; Abdul-Tehrani, Hossein; Majid, Shahana

doi:10.1016/j.diagmicrobio.2008.05.003

Cited by 71 publications

(44 citation statements)

References 22 publications

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“…In the optical assay when both internal probes matches perfectly the specific sequence, occurs the nanoparticle aggregation and the colour changes from red to purple indicating the presence of the bacterium DNA. 51 This method showed high sensitivity and specificity in comparison to histologic studies and also reduced the time and cost needed for the molecular diagnosis of H. pylori. It is possible to detect as little as 10 CFU (colony forming units) mL −1 of H. pylori in less than 1 h. AuNPs were also used to recognize and detect specific DNA sequences in a single step.…”

Section: Opticalmentioning

confidence: 99%

Biosensors based on gold nanostructures

Vidotti¹,

Carvalhal²,

Ferreira³

et al. 2011

J. Braz. Chem. Soc.

130

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O presente trabalho de revisão aborda os mais recentes avanços na tecnologia de biossensores alcançados através da montagem de biomoléculas associada com nanopartículas de ouro na construção de dispositivos analíticos. Esta revisão está dividida de acordo com a biomolécula empregada no desenvolvimento de biossensores: (i) compostos imunológicos; (ii) DNA/RNA funcionais; e (iii) enzimas e proteínas Heme. A fim de facilitar a compreensão, cada seção foi subdividida de acordo com o modo de transdução. Os imunossensores contendo nanopartículas de ouro têm uma ampla gama de aplicações nos campos alimentício, ambiental, farmacêutico, químico e de diagnósticos clínicos. As nanopartículas foram empregadas para melhorar o sinal analítico ou a imobilização dos imunocompostos. Em outra seção, os biossensores DNA/ RNA empregando nanoestruturas de ouro como labels e biossensores label-free associados a nanoestruturas de ouro como transdutores foram sistematicamente relatados para a rápida identificação de patógenos, espécies de interesse ambiental e diagnóstico clínico. A inclusão de nanopartículas de ouro em eletrodos modificados aumenta a transferência de elétrons entre o transdutor e a biomolécula proporcionando um melhor desempenho quando enzimas e proteínas redox heme são usados. Biossensores para a detecção e quantificação de glicose e peróxido de hidrogênio foram também discutidos.The present review discusses the latest advances in biosensor technology achieved by the assembly of biomolecules associated with gold nanoparticles in analytical devices. This review is divided in sections according to the biomolecule employed in the biosensor development: (i) immunocompounds; (ii) DNA/RNA and functional DNA/RNA; and (iii) enzymes and Heme proteins. In order to facilitate the comprehension each section was subdivided according to the transduction mode. Gold nanoparticles based immunosensors have a wide range of applications in food, environmental, pharmaceutical, chemistry and clinical diagnostics. The nanoparticles were employed to improve whether the analytical signal or the immunocompounds immobilization. In another section, biosensors based on DNA/RNA biomolecules employing gold nanostructures as labels and label-free funtional DNA/RNA biosensors associated to gold nanostructures as tranducers were systematically reported for rapid identification of pathogens, species of environmental interest and clinical diagnostics, respectively. The inclusion of gold nanoparticles in modified electrodes itself enhances the electron transfer between the transducer and biomolecules leading to improved bioanalytical devices when redox enzymes and heme proteins are used. Biosensors for the detection and quantification of glucose and hydrogen peroxide are discussed as well.

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Section: Opticalmentioning

confidence: 99%

Biosensors based on gold nanostructures

Vidotti¹,

Carvalhal²,

Ferreira³

et al. 2011

J. Braz. Chem. Soc.

130

View full text Add to dashboard Cite

show abstract

“…A number of exciting advances have been made in the field of nanodiagnostics largely because of advances in nanoparticle synthesis and the control over their surface binding properties [21][22][23]. On the other hand, several amplification technologies have been described in the past two decades without using thermocycler machine [6].…”

Section: Discussionmentioning

confidence: 99%

“…For the evaluation of the specificity, non-target nucleic acids, notably yeast tRNA and total E. coli RNA, were added, alone or in the presence of M. tuberculosis 16S rRNA and analyzed by the assay [12,21].…”

Section: Validation Of the Colorimetric Assaymentioning

confidence: 99%

Nanodiagnostic Method for Colorimetric Detection of Mycobacterium tuberculosis 16S rRNA

et al. 2008

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A nanodiagnostic method using nucleic acid sequence-based amplification (NASBA) and gold nanoparticle probes (AuNP probes) was developed for colorimetric detection of Mycobacterium tuberculosis. The primers targeting 16S rRNA were used for the amplification of mycobacterial RNA by the isothermal NASBA process. The amplicons were hybridized with specific gold nanoparticle probes. The RNA-DNA hybrids were colorimetrically detected by the accumulation of gold nanoparticles. Using this method, 10 CFU ml −1 of M. tuberculosis was detected within less than 1 h. Results obtained from the clinical specimens showed 94.7% and 96% sensitivity and specificity, respectively. No interference was encountered in the amplification and detection of M. tuberculosis in the presence of non-target bacteria, confirming the specificity of the method.

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“…Thermophilic HDA was also used for the detection of Helicobacter pylori in combination with ELISA using a digoxigenin-labeled primer by Gill and colleagues. In addition, they also developed a colorimetric method to detect H. pylori using thermophilic HDA and gold nonoparticles [77,78]. It has been reported that RNA targets as well as DNA were also amplified and detected by .…”

Section: Helicase-dependent Dna Amplificationmentioning

confidence: 99%

Isothermal DNA amplification in vitro: the helicase-dependent amplification system

Jeong

Park

Kim

2009

Cell. Mol. Life Sci.

118

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Since the development of polymerase chain reaction, amplification of nucleic acids has emerged as an elemental tool for molecular biology, genomics, and biotechnology. Amplification methods often use temperature cycling to exponentially amplify nucleic acids; however, isothermal amplification methods have also been developed, which do not require heating the double-stranded nucleic acid to dissociate the synthesized products from templates. Among the several methods used for isothermal DNA amplification, the helicase-dependent amplification (HDA) is discussed in this review with an emphasis on the reconstituted DNA replication system. Since DNA helicase can unwind the double-stranded DNA without the need for heating, the HDA system provides a very useful tool to amplify DNA in vitro under isothermal conditions with a simplified reaction scheme. This review describes components and detailed aspects of current HDA systems using Escherichia coli UvrD helicase and T7 bacteriophage gp4 helicase with consideration of the processivity and efficiency of DNA amplification.

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Colorimetric detection of Helicobacter pylori DNA using isothermal helicase-dependent amplification and gold nanoparticle probes

Cited by 71 publications

References 22 publications

Biosensors based on gold nanostructures

Biosensors based on gold nanostructures

Nanodiagnostic Method for Colorimetric Detection of Mycobacterium tuberculosis 16S rRNA

Isothermal DNA amplification in vitro: the helicase-dependent amplification system

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