Colorimetric detection of endogenous hydrogen sulfide production in living cells

Ahn, Youngjun; Lee, Young Ju; Lee, Jaemyeon; Lee, Doyeon; Park, Hun-Kuk; Lee, Gi-Ja

doi:10.1016/j.saa.2017.01.040

Cited by 37 publications

(16 citation statements)

References 30 publications

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“…A microplate colorimetric H 2 S detection assay designed for H 2 S measurement was used as previously described [35] , [36] . Briefly, poly vinyl pyrrolidone (PVP, 5% w/v) solution was added to Nafion® perfluorinated resin solution in a ratio of 9:1 (v/v).…”

Section: Methodsmentioning

confidence: 99%

Targeting hydrogen sulphide signaling in breast cancer

Youness

Gad

Sanber

et al. 2021

Journal of Advanced Research

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Section: Methodsmentioning

confidence: 99%

Targeting hydrogen sulphide signaling in breast cancer

Youness

Gad

Sanber

et al. 2021

Journal of Advanced Research

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“…The most obvious method to increase H 2 S levels in the body, of course, is by "feeding" the endogenous H 2 S producing enzymes with its substrates: L-cysteine and L-homocysteine for CSE and CBS, 3-mercaptopyruvate for 3-MST, and a-ketoglutarate for CAT (which, in turn, produces 3-MP). Although it is generally assumed that cells contain saturating concentrations of these substrates and, therefore, exogenous supplementation would not have a marked effect, in fact, in vitro and in vivo studies demonstrate that these three substrates can increase H 2 S levels, in a fashion that is reduced by inhibitors of the respective H 2 S-producing enzymes (e.g., Kartha et al, 2012;Ahn et al, 2017;, and induce biologic effects that are consistent with elevation of H 2 S production, such as smooth muscle relaxation (d'Emmanuele di Villa Bianca et al, 2009;Flannigan et al, 2013;Yamane et al, 2015;Prieto-Lloret and Aaronson, 2015;Kuo et al, 2016;Yetik-Anacak et al, 2016), cell proliferation and angiogenesis (Wang et al, 2013c;Coletta et al, 2015), organ protection (Elsey et al, 2010;Magierowski et al, 2017) and bell-shaped effects on mitochondrial function (stimulation at lower concentrations and inhibition at higher concentrations) (Modis et al, 2013a,b). In some tissues, D-cysteine can also yield H 2 S, through actions on the D-amino acid oxidase, with beneficial/cytoprotective results Souza et al, 2017).…”

Section: Alternative Means To Increase Biologic H 2 S Levelsmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. CII: Pharmacological Modulation of H₂S Levels: H₂S Donors and H₂S Biosynthesis Inhibitors

2017

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Over the last decade, hydrogen sulfide (HS) has emerged as an important endogenous gasotransmitter in mammalian cells and tissues. Similar to the previously characterized gasotransmitters nitric oxide and carbon monoxide, HS is produced by various enzymatic reactions and regulates a host of physiologic and pathophysiological processes in various cells and tissues. HS levels are decreased in a number of conditions (e.g., diabetes mellitus, ischemia, and aging) and are increased in other states (e.g., inflammation, critical illness, and cancer). Over the last decades, multiple approaches have been identified for the therapeutic exploitation of HS, either based on HS donation or inhibition of HS biosynthesis. HS donation can be achieved through the inhalation of HS gas and/or the parenteral or enteral administration of so-called fast-releasing HS donors (salts of HS such as NaHS and NaS) or slow-releasing HS donors (GYY4137 being the prototypical compound used in hundreds of studies in vitro and in vivo). Recent work also identifies various donors with regulated HS release profiles, including oxidant-triggered donors, pH-dependent donors, esterase-activated donors, and organelle-targeted (e.g., mitochondrial) compounds. There are also approaches where existing, clinically approved drugs of various classes (e.g., nonsteroidal anti-inflammatories) are coupled with HS-donating groups (the most advanced compound in clinical trials is ATB-346, an HS-donating derivative of the non-steroidal anti-inflammatory compound naproxen). For pharmacological inhibition of HS synthesis, there are now several small molecule compounds targeting each of the three HS-producing enzymes cystathionine--synthase (CBS), cystathionine--lyase, and 3-mercaptopyruvate sulfurtransferase. Although many of these compounds have their limitations (potency, selectivity), these molecules, especially in combination with genetic approaches, can be instrumental for the delineation of the biologic processes involving endogenous HS production. Moreover, some of these compounds (e.g., cell-permeable prodrugs of the CBS inhibitor aminooxyacetate, or benserazide, a potentially repurposable CBS inhibitor) may serve as starting points for future clinical translation. The present article overviews the currently known HS donors and HS biosynthesis inhibitors, delineates their mode of action, and offers examples for their biologic effects and potential therapeutic utility.

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“…23 summarises the different fluorescent probes for measurement of sulfide. Measurement of sulfide in fluids 14,24–26 and living cells 13,27–29 has been usually performed using bulk sample making it unwieldy for clinical use. However, by miniaturizing the detection and measurement system, it would be possible to usher in point of care devices for gasotransmitters.…”

Section: Introductionmentioning

confidence: 99%

Rapid measurement of hydrogen sulphide in human blood plasma using a microfluidic method

Karunya

Jayaprakash

Gaikwad

et al. 2019

Sci Rep

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Hydrogen sulfide (H 2 S) is emerging as an important gasotransmitter in both physiological and pathological states. Rapid measurement of H 2 S remains a challenge. We report a microfluidic method for rapid measurement of sulphide in blood plasma using Dansyl-Azide, a fluorescence (FL) based probe. We have measured known quantities of externally added (exogenous) H 2 S to both buffer and human blood plasma. Surprisingly, a decrease in FL intensity with increase in exogenous sulphide concentration in plasma was observed which is attributed to the interaction between the proteins and sulphide present in plasma underpinning our observation. The effects of mixing and incubation time, pH, and dilution of plasma on the FL intensity is studied which revealed that the FL assay required a mixing time of 2 min, incubation time of 5 min, a pH of 7.1 and performing the test within 10 min of sampling; these together constitute the optimal parameters at room temperature. A linear correlation (with R 2 ≥ 0.95) and an excellent match was obtained when a comparison was done between the proposed microfluidic and conventional spectrofluorometric methods for known concentrations of H 2 S (range 0–100 µM). We have measured the baseline level of endogenous H 2 S in healthy volunteers which was found to lie in the range of 70 μM – 125 μM. The proposed microfluidic device with DNS-Az probe enables rapid and accurate estimation of a key gasotransmitter H 2 S in plasma in conditions closely mimicking real time clinical setting. The availability of this device as at the point of care, will help in understanding the role of H 2 S in health and disease.

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Colorimetric detection of endogenous hydrogen sulfide production in living cells

Cited by 37 publications

References 30 publications

Targeting hydrogen sulphide signaling in breast cancer

Targeting hydrogen sulphide signaling in breast cancer

International Union of Basic and Clinical Pharmacology. CII: Pharmacological Modulation of H₂S Levels: H₂S Donors and H₂S Biosynthesis Inhibitors

Rapid measurement of hydrogen sulphide in human blood plasma using a microfluidic method

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Colorimetric detection of endogenous hydrogen sulfide production in living cells

Cited by 37 publications

References 30 publications

Targeting hydrogen sulphide signaling in breast cancer

Targeting hydrogen sulphide signaling in breast cancer

International Union of Basic and Clinical Pharmacology. CII: Pharmacological Modulation of H2S Levels: H2S Donors and H2S Biosynthesis Inhibitors

Rapid measurement of hydrogen sulphide in human blood plasma using a microfluidic method

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International Union of Basic and Clinical Pharmacology. CII: Pharmacological Modulation of H₂S Levels: H₂S Donors and H₂S Biosynthesis Inhibitors