Colorimetric detection of blood glucose based on GOx@ZIF-8@Fe-polydopamine cascade reaction

Zhao, Zhenghong; Lin, Tianran; Liu, Wenren; Hou, Li; Ye, Fanggui; Zhao, Shulin

doi:10.1016/j.saa.2019.04.061

Cited by 44 publications

(32 citation statements)

References 35 publications

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“…In recent years, metal-organic frameworks (MOFs), a kind of porous crystalline organic-inorganic hybrid materials, composing of inorganic metal ions or metal clusters and organic ligands, have emerged as an excellent carrier for enzyme immobilization because of their fascinating physicochemical properties like thermal stability, high-surface area, pore size selectivity, and biocompatibility (Tan et al, 2019;Zhao et al, 2019;Zhou et al, 2019). Several approaches including physical adsorption (Nobakht et al, 2018;Jia et al, 2019), covalent bonding (Yue et al, 2016;Nowroozi-Nejad et al, 2019), and biomimetic mineralization (Liang et al, 2015;Jiang et al, 2017) have been employed to immobilize enzymes into MOFs.…”

Section: Introductionmentioning

confidence: 99%

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Lei

Liang

Luo

2020

Front. Bioeng. Biotechnol.

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In this study, we have developed a mild and effective method to prepare a metal-organic framework (MOF)-based microcapsule by the self-assembly of pre-synthesized zeolite imidazolate framework-8 (ZIF-8) nanoparticles at the oil-water interface combined with deposition of a dense ZIF-8 coating outside the capsule. By introducing the enzyme Candida antarctica lipase B (CalB) directly into the stabilizer ZIF-8 or the water phase of Pickering emulsion during the preparation process, we achieved that the enzyme was immobilized within the shell (CalB@ZIF-8@cap) or in the cavity (ZIF-8@cap-CalB) of the microcapsules, respectively. The resulting CalB-loaded microcapsules were robust and had a core-shell structure proved by scanning electron microscopy. Meanwhile, Fourier transform infrared spectroscopy was conducted to confirm the encapsulation of enzymes in the microcapsules and their position in the microcapsules was confirmed by fluorescence microscopy. Furthermore, through the comparison of transesterification reactions between a pair of small substrates and a pair of larger ones, the two types of CalB-loaded microcapsules showed great catalytic activity, stability and size selectivity, and the catalytic activity of CalB@ZIF-8@cap was slightly higher than that of ZIF-8@cap-CalB. Importantly, due to the large size of the microcapsules, the catalyst could be separated from the reaction system by sedimentation, thereby reducing the energy consumption for separation. These kinds of multifunctional MOF-enzyme composites may open up new opportunities for the biocatalysis and microreactor.

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Section: Introductionmentioning

confidence: 99%

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Lei

Liang

Luo

2020

Front. Bioeng. Biotechnol.

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“…First, the GOx was loaded into NCPs through the coordination between FA and Zn 2+ (scheme 1 ), and the successful formation of NCPs/GOx was confirmed by TEM ( Figure S1 ), with GOx encapsulation efficiency (EE%) of 60%. Such effective GOx loading was benefited from the high porous of NCPs, strong coordination of the protein with Zn 2+ 41 , as well as the π-π stacking interactions between the molecules 41 - 43 . Within NCPs/GOx structure, both GOx (275 nm) and FA (280 nm) displayed a red-shift of the UV-vis spectrum, confirming the π-π stacking interactions (Figure 1 A) 44 .…”

Section: Resultsmentioning

confidence: 99%

Cell membrane inspired nano-shell enabling long-acting Glucose Oxidase for Melanoma starvation therapy via microneedles-based percutaneous delivery

Zeng¹,

Zhou²,

Ding³

et al. 2021

Theranostics

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Rationale: Glucose oxidase (GOx) has gained tremendous research interest recently as a glucose-consuming enzyme for tumor starvation therapy, while its in vivo applications are strictly limited by rapid deactivation, as well as side effects of non-specific catalysis. Methods: To address these issues, here we report a protective nano-shell to encapsule GOx for localized melanoma therapy delivered by dissolving microneedles (MNs). Inspired by cell membrane that separates and protects cell organelles and components from outside environment while selectively ingesting nutrition sources, we designed polydopamine (PDA)-structured nano-shell to allow free transportation of glucose for catalytic reaction, while impede the penetration of GOx, proteinase, and other GOx-deactivating macromolecules across the shell membrane. Results: GOx was well protected in core layer with persistent catalytic activity for at least 6 d under various biological matrixes ( e.g. , PBS, serum, and cell lysate) and surviving different harsh conditions ( e.g. , acid/base treatments, and proteinase-induced degradation). Such long-acting nano-catalyst can be easily integrated into MNs as topical delivery carrier for effective glucose consumption in melanoma tissue, achieving significant tumor growth inhibition via starvation therapy with minimized side effects as compared to systemic administration. Conclusion: This work provides an elegant platform for in vivo delivery of GOx, and our cell-mimicking nano-system can also be applied for other enzyme-based therapeutics.

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“…In order to further enhance the selectivity of glucose sensing, glucose oxidase (GOx) has been integrated to the MOFs through absorption, covalent linkage, pore encapsulation, and coprecipitation [300][301][302]. The MOFs/natural enzyme hybrids combined the advantages of controllable catalytic activity of the MOFs and selectivity of natural enzymes [303].…”

Section: Biomoleculesmentioning

confidence: 99%

Metal-organic frameworks as functional materials for implantable flexible biochemical sensors

Ling

Liu

et al. 2021

Nano Res.

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Metal-organic frameworks (MOFs) exhibit attractive properties such as highly accessible surface area, large porosity, tunable pore size, and built-in redox-active metal sites. They may serve as excellent candidates to construct implantable flexible devices for biochemical sensing due to their high thermal and solution stability. However, MOFs-based sensors have only been mostly reported for in-vitro chemical sensing, their use in implantable chemical sensing and combination with flexible electronics to achieve excellent mechanical compatibility with tissues and organs has rarely been summarized. This paper systematically reviews the biochemical sensors based on MOFs and discusses the feasibility to achieve implantable biochemical sensing through MOFsbased flexible electronics. The properties of MOFs and underlying mechanisms have been introduced, followed by a summarization of different biochemical sensing applications. Strategies to integrate MOFs with flexible devices have been supplied from the standpoints of matching mechanics and compatible fabrication processes. Issues that should be addressed in developing flexible MOFs sensors and potential solutions have also been provided, followed by the perspective for future applications of flexible MOFs sensors. This paper may serve as a reference to offer potential guidelines for the development of flexible MOFs-based biochemical sensors that may benefit future applications in personal healthcare, disease diagnosis and treatment, and fundamental study of various biological processes.

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Colorimetric detection of blood glucose based on GOx@ZIF-8@Fe-polydopamine cascade reaction

Cited by 44 publications

References 35 publications

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Cell membrane inspired nano-shell enabling long-acting Glucose Oxidase for Melanoma starvation therapy via microneedles-based percutaneous delivery

Metal-organic frameworks as functional materials for implantable flexible biochemical sensors

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