Colorimetric detection of 1,5-anhydroglucitol based on graphene quantum dots and enzyme-catalyzed reaction

Zhou, Zhide; Zhao, Le; Wang, Zhihong; Xue, Wen; Wang, Yunxiao; Huang, Yong; Liang, Jianwei; Chen, Jiejing; Li, Guiyin

doi:10.1016/j.ijbiomac.2018.02.093

Cited by 12 publications

(5 citation statements)

References 40 publications

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“…In this regard, these concentrations are especially appropriate since the concentrations of this biomarker in blood, urine and saliva are notoriously lower than others (1.7–243 μmol L −1 ), as presented in Table 1. In addition, the LOD reported here (0.08 μmol L −1 ) is lower than that of other similar studies with LOD of 2.98, 54 and 0.88 μmol L −1 47‐49 …”

Section: Resultscontrasting

confidence: 80%

“…In addition, the LOD reported here (0.08 μmol L −1 ) is lower than that of other similar studies with LOD of 2.98, 54 and 0.88 μmol L −1 . [47][48][49] All the buffer solutions used for this set of experiments were phosphate buffers (0.1 mol L −1 , pH 7) for galactose and 1,5-AHG HRP, horseradish peroxidase; LOD, limit of detection (3.3⊞/slope); LOQ, limit of quantification (10⊞/slope); TMB, 3,3 0 ,5,5 0 -tetramethylbenzidine.…”

Section: Quantitative Detection Of H 2 O 2 and Biomarkers In Buffermentioning

confidence: 99%

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Development of a simple and flexible enzyme‐based platform for the colorimetric detection of multiple biomarkers in non‐conventional biofluids

Ornelas‐González

Rito‐Palomares

2022

J of Chemical Tech & Biotech

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Background Enzyme‐based colorimetric systems are inexpensive, simple, adaptable and sensitive methods that allow specific quantification of a substrate. In the clinical field, their use has been the basis for the development of equipment and devices that today are key for disease detection. The objective of this project was to demonstrate the flexibility of a previously developed multi‐enzyme system for colorimetric glucose quantification by adapting and optimizing it for the detection and quantification of other clinically relevant biomolecules, such as galactose, uric acid and 1,5‐anhydroglucitol in buffer conditions. Results The obtained calibration curves for galactose, uric acid and 1,5‐anhydroglucitol show remarkable linearity (R2 ≥ 0.997), precision (CV ≤ 2.38%) and sensitivity with detection and quantification limits of 3.96 and 12.01 μmol L−1; 0.16 and 0.48 μmol L−1; and 0.08 and 0.25 μmol L−1, respectively. In addition, it was found that the three systems are capable of quantifying different concentrations of their respective substrate, showing low variability (CV < 3.3%) and notable recovery percentages (99.21–103.39%). Conclusions The results obtained with the three optimized enzyme‐based colorimetric systems demonstrate the platform flexibility by modifying parameters such as pH buffer, incubation time, enzyme and concentration of the reducing agent under buffer conditions. All results together demonstrate the great potential of this multi‐enzyme platform for the quantification of various substrates in non‐conventional biofluids. © 2022 Society of Chemical Industry (SCI).

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Section: Resultscontrasting

confidence: 80%

Section: Quantitative Detection Of H 2 O 2 and Biomarkers In Buffermentioning

confidence: 99%

Development of a simple and flexible enzyme‐based platform for the colorimetric detection of multiple biomarkers in non‐conventional biofluids

Ornelas‐González

Rito‐Palomares

2022

J of Chemical Tech & Biotech

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“…Z Zhou et al. ( 23 ) adopted a quantitative study for the colorimetric detection of serum 1,5-AG based on graphene quantum dots and enzyme-catalyzed reactions. Their findings indicated a linear correlation between the absorbance and the concentration of serum 1,5-AG in the range of 20.0–100.0 μg/mL, with a detection limit of approximately 0.144 μg/mL.…”

Section: Overview Of 15-agmentioning

confidence: 99%

The progress of clinical research on the detection of 1,5-anhydroglucitol in diabetes and its complications

Xu,

Pan,

Chen

2024

Front. Endocrinol.

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1,5-Anhydroglucitol (1,5-AG) is sensitive to short-term glucose fluctuations and postprandial hyperglycemia, which has great potential in the clinical application of diabetes as a nontraditional blood glucose monitoring indicator. A large number of studies have found that 1,5-AG can be used to screen for diabetes, manage diabetes, and predict the perils of diabetes complications (diabetic nephropathy, diabetic cardiovascular disease, diabetic retinopathy, diabetic pregnancy complications, diabetic peripheral neuropathy, etc.). Additionally, 1,5-AG and β cells are also associated with each other. As a noninvasive blood glucose monitoring indicator, salivary 1,5-AG has much more benefit for clinical application; however, it cannot be ignored that its detection methods are not perfect. Thus, a considerable stack of research is still needed to establish an accurate and simple enzyme assay for the detection of salivary 1,5-AG. More clinical studies will also be required in the future to confirm the normal reference range of 1,5-AG and its role in diabetes complications to further enhance the blood glucose monitoring system for diabetes.

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“…Several methods have been explored to detect the level of serum 1,5-AG in a sensitive and accurate way. Routine techniques, such as the enzymatic method, chromatography, colourimetric assay, and mass spectrometry, have been used in clinical practice [ 4 , 9 , 10 , 11 , 12 , 13 ]. Currently, the Glycomark TM assay, approved by the United States Federal Drug Administration in 2003, is regarded as the gold standard to determine serum 1,5-AG levels [ 11 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

An Efficient Electrochemical Biosensor to Determine 1,5-Anhydroglucitol with Persimmon-Tannin-Reduced Graphene Oxide-PtPd Nanocomposites

Zhou

Wang

et al. 2023

Materials

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1,5-Anhydroglucitol (1,5-AG) is a sensitive biomarker for real-time detection of diabetes mellitus. In this study, an electrochemical biosensor to specifically detect 1,5-AG levels based on persimmon-tannin-reduced graphene oxide-PtPd nanocomposites (PT-rGO-PtPd NCs), which were modified onto the surface of a screen-printed carbon electrode (SPCE), was designed. The PT-rGO-PtPd NCs were prepared by using PT as the film-forming material and ascorbic acid as the reducing agent. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), ultraviolet–visible spectroscopy (UV–vis), and X-ray diffraction (XRD) spectroscopy analysis were used to characterise the newly synthesised materials. PT-rGO-PtPd NCs present a synergistic effect not only to increase the active surface area to bio-capture more targets, but also to exhibit electrocatalytic efficiency to catalyze the decomposition of hydrogen peroxide (H2O2). A sensitive layer is formed by pyranose oxidase (PROD) attached to the surface of PT-rGO-PtPd NC/SPCE. In the presence of 1,5-AG, PROD catalyzes the oxidization of 1,5-AG to generate 1,5-anhydrofuctose (1,5-AF) and H2O2 which can be decomposed into H2O under the synergistic catalysis of PT-rGO-PtPd NCs. The redox reaction between PT and its oxidative product (quinones, PTox) can be enhanced simultaneously by PT-rGO-PtPd NCs, and the current signal was recorded by the differential pulse voltammetry (DPV) method. Under optimal conditions, our biosensor shows a wide range (0.1–2.0 mg/mL) for 1,5-AG detection with a detection limit of 30 μg/mL (S/N = 3). Moreover, our electrochemical biosensor exhibits acceptable applicability with recoveries from 99.80 to 106.80%. In summary, our study provides an electrochemical method for the determination of 1,5-AG with simple procedures, lower costs, good reproducibility, and acceptable stability.

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Colorimetric detection of 1,5-anhydroglucitol based on graphene quantum dots and enzyme-catalyzed reaction

Cited by 12 publications

References 40 publications

Development of a simple and flexible enzyme‐based platform for the colorimetric detection of multiple biomarkers in non‐conventional biofluids

Development of a simple and flexible enzyme‐based platform for the colorimetric detection of multiple biomarkers in non‐conventional biofluids

The progress of clinical research on the detection of 1,5-anhydroglucitol in diabetes and its complications

An Efficient Electrochemical Biosensor to Determine 1,5-Anhydroglucitol with Persimmon-Tannin-Reduced Graphene Oxide-PtPd Nanocomposites

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