Colorimetric cellulose-based test-strip for rapid detection of amyloid β-42

Moreira, Felismina T.C.; Correia, Barbara P.; Sousa, Mariana P.; Sales, Goreti

doi:10.1007/s00604-021-04996-7

Cited by 8 publications

(2 citation statements)

References 53 publications

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“…Colorimetric paper-based sensors have emerged in the diagnosis of different diseases including cancer [22], neurodegenerative [52], infectious [53], and other chronic diseases [54].…”

Section: Colorimetric Transductionmentioning

confidence: 99%

Colorimetric Paper-Based Sensors against Cancer Biomarkers

Carneiro

Rodrigues

Moreira

et al. 2022

Sensors

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Cancer is a major cause of mortality and morbidity worldwide. Detection and quantification of cancer biomarkers plays a critical role in cancer early diagnosis, screening, and treatment. Clinicians, particularly in developing countries, deal with high costs and limited resources for diagnostic systems. Using low-cost substrates to develop sensor devices could be very helpful. The interest in paper-based sensors with colorimetric detection increased exponentially in the last decade as they meet the criteria for point-of-care (PoC) devices. Cellulose and different nanomaterials have been used as substrate and colorimetric probes, respectively, for these types of devices in their different designs as spot tests, lateral-flow assays, dipsticks, and microfluidic paper-based devices (μPADs), offering low-cost and disposable devices. However, the main challenge with these devices is their low sensitivity and lack of efficiency in performing quantitative measurements. This review includes an overview of the use of paper for the development of sensing devices focusing on colorimetric detection and their application to cancer biomarkers. We highlight recent works reporting the use of paper in the development of colorimetric sensors for cancer biomarkers, such as proteins, nucleic acids, and others. Finally, we discuss the main advantages of these types of devices and highlight their major pitfalls.

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“…Colorimetric paper-based sensors have emerged in the diagnosis of different diseases including cancer [22], neurodegenerative [52], infectious [53], and other chronic diseases [54].…”

Section: Colorimetric Transductionmentioning

confidence: 99%

Colorimetric Paper-Based Sensors against Cancer Biomarkers

Carneiro

Rodrigues

Moreira

et al. 2022

Sensors

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“…In this context, matrix metalloproteinases have become one important focus in biosensor development and extensively studied for their roles in cancer-related pathologies, in the past decades. − In fact, matrix metalloproteinases play a role in a wide variety of physiologic processes, such as the pathogenesis of different diseases including inflammation, tumor growth, and cancer metastasis. , The matrix metalloproteinase 7 (MMP7), a calcium-dependent zinc-containing extracellular protease, has been reported as a biomarker for early pancreatic cancer (PC) prediction and a noninvasive surrogate biomarker of pancreatic inflammation, being an important signaling biomarker of the tumor progression and follow-up of the disease after surgery. ,, Several approaches currently available for the detection of MMP7 levels include enzyme-linked immunosorbent assay (ELISA), Western blot analysis, and colorimetric − and fluorescence-based assays − as well as electrochemical processes. , Despite their sensitivity and accuracy, these techniques require extensive protocols to obtain the result. In line with this, point-of-care tests (PoCTs) focus on the quantification of several biological targets in a user-friendly perspective, taking advantage of the accuracy of the biorecognition method and the sensitivity of the corresponding technique. − In this context, molecularly imprinted polymers (MIPs) arise as new biomimetic recognition units to simulate the antigen antibodies interactions, already being used in the quantification of several biological targets with the advantage that can be adapted to PoCTs. − These three-dimensional structures are based on polymeric imprinting that mimics the structure and conformation of the target, thus acting as its biomimetic recognition element. MIPs possess higher chemical stability at wider pH ranges and temperatures, comparatively to immuno-based recognition methods and usually prepared with controlled size polymerization in aqueous media for the imprinting of large biomolecules as proteins. − Surface imprinting using free radical polymerization (FRP) techniques is one possible strategy for the assembly of MIPs around biological molecules, as it requires soft polymerization conditions in aqueous media. − Quantum dots (QDs) are semiconducting nanocrystals that find application as optical sensing probes.…”

Section: Introductionmentioning

confidence: 99%

Development of a Sensitive Ratiometric Imprinted Hydrogel for the Detection of Matrix Metalloproteinase 7 (MMP7) Biomarker

Piloto,

Ribeiro,

Santos

et al. 2023

ACS Appl. Opt. Mater.

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A dual-emissive fluorescent probe was developed for the sensitive and selective detection of matrix metalloproteinase 7 (MMP7), a protein biomarker associated with pancreatic cancer. The ratiometric probe consisting of blue emitting carbon dots (CDs) and molecularly imprinted polymers (MIPs) previously assembled around red emitting quantum dots (QDs) was successfully combined for the detection of MMP7 protein. The concept is to use MIPs that function as the biorecognition elements, conjugated to cadmium telluride QDs, as the sensing system. The fluorescence intensity of red QDs is quenched with increasing concentrations of the analyte, acting as sensitive probes, while the fluorescence intensity of blue emitting CDs remains constant, acting as internal controls. The resultant fluorescence color changed from red to blue as a function of the MMP7 concentration, under a 365 nm UV lamp. The imprinted material MIP@QDs were successfully incorporated within a cellulose hydrogel containing CDs as reference probes. The resultant materials were designated as imprinted ratiometric hydrogels (imprinted rHGs). The fluorescence quenching of the imprinted rHGs occurred with increasing concentrations of MMP7, showing linearity in the range [1.49 × 10 −11 − 1.92 × 10 −9 ] g/mL, in 1000-fold diluted human serum. The imprinted rHGs showed an imprinting factor of 1.83 and a limit of detection of 4.11 × 10 −12 g/mL. Overall, the imprinted rHGs developed in this work presented increased selectivity for the MMP7 protein over red emitting QDs nanoparticles and higher sensitivity comparatively to the non-imprinted rHGs.

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