Colorimetric‐based detection of Ureaplasma urealyticum using gold nanoparticles

Abstract

Ureaplasma urealyticum (uu) is one of the most common agents of urogenital infections and is associated with complications such as infertility, spontaneous abortion and other sexually transmitted diseases. Here, a DNA sensor based on oligonucleotide target-specific gold nanoparticles (AuNPs) was developed, in which the dispersed and aggregated states of oligonucleotide-functionalised AuNPs were optimised for the colorimetric detection of a polymerase chain reaction (PCR) amplicon of U. urealyticum DNA. A non-cross-linking approach utilising a single Au-nanoprobe specific of the urease gene was utilised and the effect of a PCR product concentration gradient evaluated. Results from both visual and spectral analyses showed that target-Au-nanoprobe hybrids were stable against aggregation after adding the inducer. Furthermore, when a nontarget PCR product was used, the peak position shifted and salt-induced aggregation occurred. The assay's limit of detection of the assay was 10 ng with a dynamic range of 10-60 ng. This procedure provides a rapid, facile and low-cost detection format, compared to methods currently used for the identification of U. urealyticum.

Design of a gold nanoprobe for the detection ofPseudomonas aeruginosaelastase gene (lasB)

“…In 2020, Nahid Ghorbanzadeh et al using a gold nanoprobe and the NCL approach were able to identify the urease amplied gene related to ureaplasma urealyticum. 44 In their study, a 23-nucleotide probe with 6 nucleotide spacers was used. In their study, as in our study, MgCl 2 salt was used for the induction of aggregation and the probe used in their study had a spacer like our probe.…”