Colorimetric and titrimetric assay of isoniazid

El-Brashy, A.; El‐Ashry, Saadia M.

doi:10.1016/0731-7085(92)80060-z

Cited by 46 publications

(19 citation statements)

References 17 publications

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“…This has prompted many investigators to plan methods for the rapid determination of INH in its pure form as well as in pharmaceutical preparations. There are various analytical procedures for the assay of INH, the most important being titrimetry [9,10], visible spectrophotometry [11][12][13], polarography [14], coulometry [15], high-performance liquid chromatography [16], and fluorimetry [17] methods. The spectrophotometric methods involve the use of reagents such as chloranil [18], 4-nitrobenzaldehyde, pyridoxal [19], 4-dimethylaminobenzaldehyde [20], and so forth.…”

Section: Introductionmentioning

confidence: 99%

Novel Reagents for the Spectrophotometric Determination of Isoniazid

2012

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Isoniazid is an antitubercular drug, widely used for tuberculosis. Owing to its importance in therapeutics, the present study aims to develop simple method for the spectrophotometric determination of isoniazid (INH). Two novel reagents, epichlorohydrine (ECH) and 4-hydroxyphenaylchloride (HPC) are used for the spectrophotometric determination of INH. Based on the nucleophilic substitution reactions of INH with EPI & HPC in basic medium, rapid, simple, inexpensive, precise, and accurate visible spectrophotometric method is proposed for the determination of INH in bulk drug and in formulations. Method involves the reaction of INH with EPI and HPC in basic medium to form yellow-colored chromogen, measuring the absorbances at 405 and 402 nm for INH-EPI & INH-HPC, respectively. The optimum experimental conditions have been studied. The absorbance was found to increase linearly with the concentration of the drug and formed the basis for quantification. The calibration graphs were linear from 2.00–22.00 μg mL−1and 20.00–120.00 μg mL−1for INH-EPI & INH-HPC, respectively. The apparent molar absorptivity and Sandell's sensitivity are calculated to be0.51×104&0.10×104 L mol−1 cm−1and 0.027 & 0.134 μg cm−2for INH-EPI & INH-HPC, respectively. The procedure is used to determine INH in pharmaceutical products. The associated pharmaceutical materials do not interfere in the measurements.

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Section: Introductionmentioning

confidence: 99%

Novel Reagents for the Spectrophotometric Determination of Isoniazid

2012

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“…including titrimetry [24], spectrophotometry [25] [26], high performance liquid chromatography (HPLC) [27], chemiluminescence [28], fluorimetry [29], capillary electrophoresis [30] and electroanalytical methods [31]. Among these approaches, electroanalytical techniques are of particular advantage because of their practicality, simplicity, low-cost, good sensitivity, precision and rapidity for real-time detection.…”

Section: Many Analytical Methods Have Been Developed For the Determinmentioning

confidence: 99%

A highly sensitive electrochemical determination of Isoniazid in pharmaceutical preparation by differential pulse Polarography

AL-Ameri¹,

AL-Mayahi²

2016

IJCER

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A simple strategy and highly sensitive electrochemical method was developed and described in Britton _ Robinson buffer for isoniazid determination by Differential Pulse Polarography (DPP). A dropping mercury electrode (DME) was used to characterize the performance of the sensor. The reduction process on the (DME) gave a rise with one peak over E p-1.17 v (vs. Ag/AgCl/), within the studied pH range (2-9), 3M KNO 3 as a supporting electrolyte and 4mm 3 mercury drop size at 25°C. A standard calibration plot was conducted in the range between 0.05-0.40μg.ml-1. The monitored differential pulse current was directly proportional to the concentration of Isoniazid and it showed a linear response in the studied range (correlation coefficient (r) = 0.9997) and the detection limit was 0.05µg. ml-1. This method effectively applied in commercial Isoniazid pharmaceuticals.

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“…Therefore, there is scope to develop a simple, rapid and robust method for the sensitive determination of INZ in various pharmaceutical samples. As per previous literature reports, INZ was analysed by using high performance liquid chromatography [4], capillary electrophoresis [5], fluorimetry [6], titrimetry [7,8], chemiluminescence [9] and electrochemical methods [10]. Electrochemical methods showed superior advantages like rapid, low-cost instrumentation, less time-consuming analyses and simple sample preparation, with reduced chemical consumption [11].…”

Section: Introductionmentioning

confidence: 99%

An Enzyme-Induced Novel Biosensor for the Sensitive Electrochemical Determination of Isoniazid

Chokkareddy

Bhajanthri

2017

Biosensors

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Abstract:In this present work, a glassy carbon electrode (GCE) was modified primarily with multiwalled carbon nanotubes (MWCNTs) and a composite of MWCNTs and titanium oxide nanoparticles (TiO 2 NPs). The enzyme horseradish peroxidase (HRP) was immobilized to enhance the sensing ability of GCE. The proposed biosensor was used for the sensitive determination of isoniazid (INZ) in various pharmaceutical samples. The electrochemical behaviour of the developed MWCNT-TiO 2 NPs-HRP-GCE biosensor was studied by using cyclic voltammetry (CV) and differential pulse voltammetric (DPV) techniques. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetry (TGA) and transmission electron microscopy (TEM) techniques were used to characterize the developed sensor. Phosphate buffer solution (PBS) with pH 7 was used as supporting electrolyte in the present investigation. The cyclic voltammetric results revealed that the increment of anodic peak currents for the enzyme-induced sensor was almost 8-fold greater than that of a bare GCE. The DPV technique exhibited good limit of detection and limit of quantification values, viz., 0.0335 µM and 0.1118 µM, respectively. Moreover, the developed sensor showed long-lasting stability and repeatability without any interferents. This strongly indicates that the fabricated sensor shows outstanding electrochemical performance towards INZ, with excellent selectivity and sensitivity. The developed sensor was successfully applied to pharmaceutical samples and gave good percentages of recoveries.

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Colorimetric and titrimetric assay of isoniazid

Cited by 46 publications

References 17 publications

Novel Reagents for the Spectrophotometric Determination of Isoniazid

Novel Reagents for the Spectrophotometric Determination of Isoniazid

A highly sensitive electrochemical determination of Isoniazid in pharmaceutical preparation by differential pulse Polarography

An Enzyme-Induced Novel Biosensor for the Sensitive Electrochemical Determination of Isoniazid

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