It is important to prepare a suitable microsurgical environment for vascular anastomosis or nerve suture. The background sheet is a useful element of this preparation, as it prevents the tissue and nylon thread from sticking and it can pull the sutured nylon thread. Various types of commercially produced background sheets are used according to the surgeon's preference. In order to investigate the parameters important in background sheets, we emailed a questionnaire to our office staff. The variables included color, elasticity, thickness, availability, workability, and cost. In particular, most respondents pointed out the importance of color. Several papers have described the materials or fashion of background sheets, but no studies have discussed their color. The purpose of our study was to investigate the optimal color of background sheets for microsurgery in reference to chromatic papers. We investigated the visibility of objects due to differences in the color of background on chromatic papers. We prepared a figure in which background sheets of different colors were lined up to compare the visibility of objects. To enhance the visibility of black nylon thread, colors with high luminance were deemed suitable for the background sheet. Additionally, green or blue was appropriate as these colors made the borders of objects (pink vessels) clearly visible and reduced a microsurgeon's discomfort in gazing upon objects. Thus, high-luminance green or blue was the optimal color of background sheets for microsurgery. Several papers have described the materials or fashion of background sheets. The materials include silicone, plastic, rubber, polyethylene, and polypropylene. The category