Colony-Stimulating Factors in the Prevention and Management of Infectious Diseases

Page, Andrea V.; Liles, W. Conrad

doi:10.1016/j.idc.2011.07.007

Cited by 20 publications

(14 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…G-CSF is also used to prevent or shorten neutropenia in chemotherapy-induced or primary congenital neutropenia [42], and mobilize hematopoietic stem cells to peripheral blood for transplantation [43]. Probably because of the anti-apoptotic and anti-inflammatory effects, G-CSF has also been used to treat nonhematopoietic targets including cerebral ischemia [44], spinal cord ischemia [45], infarct heart [46], and end stage liver disease [47].…”

Section: Discussionmentioning

confidence: 99%

Erythrocytic Mobilization Enhanced by the Granulocyte Colony-Stimulating Factor Is Associated with Reduced Anthrax-Lethal-Toxin-Induced Mortality in Mice

et al. 2014

View full text Add to dashboard Cite

Anthrax lethal toxin (LT), one of the primary virulence factors of Bacillus anthracis, causes anthrax-like symptoms and death in animals. Experiments have indicated that levels of erythrocytopenia and hypoxic stress are associated with disease severity after administering LT. In this study, the granulocyte colony-stimulating factor (G-CSF) was used as a therapeutic agent to ameliorate anthrax-LT- and spore-induced mortality in C57BL/6J mice. We demonstrated that G-CSF promoted the mobilization of mature erythrocytes to peripheral blood, resulting in a significantly faster recovery from erythrocytopenia. In addition, combined treatment using G-CSF and erythropoietin tended to ameliorate B. anthracis-spore-elicited mortality in mice. Although specific treatments against LT-mediated pathogenesis remain elusive, these results may be useful in developing feasible strategies to treat anthrax.

show abstract

Section: Discussionmentioning

confidence: 99%

Erythrocytic Mobilization Enhanced by the Granulocyte Colony-Stimulating Factor Is Associated with Reduced Anthrax-Lethal-Toxin-Induced Mortality in Mice

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Some authors have reported enhancement of neutrophil functions in terms of phagocytosis, superoxide anion generation, expression of CD11b/CD18 and bacterial killing (39–42), while others report reduced chemotaxis and altered expression of surface markers (43–45). In general, treatment with the non-pegylated forms of rG-CSF has been shown to result in a modest decrease in the incidence of febrile neutropenia, with no significant impact on overall survival (46). Few studies have been conducted with pegfilgrastim to assess neutrophil function.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Neutrophils in Mice Exposed to Radiation and/or Granulocyte Colony-Stimulating Factor Treatment

et al. 2013

View full text Add to dashboard Cite

Astronauts have the potential to develop the hematopoietic syndrome as a result of exposure to radiation from a solar particle event (SPE) during exploration class missions. This syndrome is characterized by a reduction in the number of circulating blood cells (cytopenias). In the present study the effects of SPE-like proton and γ radiation on the kinetics of circulating neutrophils were evaluated during a one-month time period using mice as a model system. The results revealed that exposure to a 2 Gy dose of either SPE-like proton or γ radiation significantly decreased the number of circulating neutrophils, with two nadirs observed on day 4 and day 16 postirradiation. Low circulating neutrophil count (neutropenia) is particularly important because it can increase the risk of astronauts developing infections, which can compromise the success of the mission. Thus, two granulocyte colony-stimulating factors (G-CSFs), filgrastim and pegfilgrastim were evaluated as countermeasures for this endpoint. Both forms of G-CSF significantly increased neutrophil counts in irradiated mice, however, the effect of pegfilgrastim was more potent and lasted longer than filgrastim. Using the expression of CD11b, CD18 and the production of reactive oxygen species (ROS) as markers of neutrophil activation, it was determined that the neutrophils in the irradiated mice treated with pegfilgrastim were physiologically active. Thus, these results suggest that pegfilgrastim could be a potential countermeasure for the reduced number of circulating neutrophils in irradiated animals.

show abstract

“…GM-CSF has recently been ascribed a role in immunity against pathogens via its action in stimulating production of granulocytes and monocytes/macrophages, as well as dendritic cell subsets [32]. GM-CSF enhances innate host defenses against microbial pathogens, and increases absolute numbers of circulating innate immune effector cells by accelerating bone marrow production and maturation [33]. As GM-CSF acts on bone marrow cells via hematopoiesis, its activity in our inflammation model implies that GM-CSF, and possibly other genes, may be at work locally in the bulla bone/bone marrow of the ear.…”

Section: Discussionmentioning

confidence: 99%

Otitis Media Impacts Hundreds of Mouse Middle and Inner Ear Genes

et al. 2013

View full text Add to dashboard Cite

ObjectiveOtitis media is known to alter expression of cytokine and other genes in the mouse middle ear and inner ear. However, whole mouse genome studies of gene expression in otitis media have not previously been undertaken. Ninety-nine percent of mouse genes are shared in the human, so these studies are relevant to the human condition.MethodsTo assess inflammation-driven processes in the mouse ear, gene chip analyses were conducted on mice treated with trans-tympanic heat-killed Hemophilus influenza using untreated mice as controls. Middle and inner ear tissues were separately harvested at 6 hours, RNA extracted, and samples for each treatment processed on the Affymetrix 430 2.0 Gene Chip for expression of its 34,000 genes.ResultsStatistical analysis of gene expression compared to control mice showed significant alteration of gene expression in 2,355 genes, 11% of the genes tested and 8% of the mouse genome. Significant middle and inner ear upregulation (fold change >1.5, p<0.05) was seen in 1,081 and 599 genes respectively. Significant middle and inner ear downregulation (fold change <0.67, p<0.05) was seen in 978 and 287 genes respectively. While otitis media is widely believed to be an exclusively middle ear process with little impact on the inner ear, the inner ear changes noted in this study were numerous and discrete from the middle ear responses. This suggests that the inner ear does indeed respond to otitis media and that its response is a distinctive process. Numerous new genes, previously not studied, are found to be affected by inflammation in the ear.ConclusionWhole genome analysis via gene chip allows simultaneous examination of expression of hundreds of gene families influenced by inflammation in the middle ear. Discovery of new gene families affected by inflammation may lead to new approaches to the study and treatment of otitis media.

show abstract

Colony-Stimulating Factors in the Prevention and Management of Infectious Diseases

Cited by 20 publications

References 89 publications

Erythrocytic Mobilization Enhanced by the Granulocyte Colony-Stimulating Factor Is Associated with Reduced Anthrax-Lethal-Toxin-Induced Mortality in Mice

Erythrocytic Mobilization Enhanced by the Granulocyte Colony-Stimulating Factor Is Associated with Reduced Anthrax-Lethal-Toxin-Induced Mortality in Mice

Kinetics of Neutrophils in Mice Exposed to Radiation and/or Granulocyte Colony-Stimulating Factor Treatment

Otitis Media Impacts Hundreds of Mouse Middle and Inner Ear Genes

Contact Info

Product

Resources

About