Colony-stimulating factor-1 receptor protein expression is a specific marker for goldfish (Carassius auratus L.) macrophage progenitors and their differentiated cell types

Katzenback, Barbara A.; Belosevic, Miodrag

doi:10.1016/j.fsi.2011.12.003

Cited by 26 publications

(9 citation statements)

References 40 publications

(51 reference statements)

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“…The effect of clodronate-liposomes treatment on ayu monocyte/macrophage depletion was studied by ip injection with clodronate-liposomes or PBS-liposomes on Days -4 and -2. In this experiment, the clodronate-liposomes were given ip at a dose of 0.25 ml/100 g. CD115, also known as colony-stimulating factor-1 receptor (CSF-1R), has been identified as the marker of monocytes/macrophages in mammal and teleost [33]. The depletion of monocytes/macrophages was confirmed by flow cytometry analysis of the CD115-positive cells in the head kidney and blood on Day 0.…”

Section: In Vivo Monocyte/macrophage Depletionmentioning

confidence: 91%

“…CD115 is identified as marker of monocytes/macrophages in teleosts [33,34]. Here, a peptide derived from extracellular domain of CD115 was chemically synthesized and subsequently used to immunize mice to prepare polyclonal antibodies, which can recognize CD115 in ayu monocytes/macrophages (Fig.…”

Section: Effect Of Liposome Injection On the Depletion Of Ayu Monocytmentioning

confidence: 99%

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LECT2 improves the outcomes in ayu with Vibrio anguillarum infection via monocytes/macrophages

Chen

et al. 2014

Fish & Shellfish Immunology

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Section: In Vivo Monocyte/macrophage Depletionmentioning

confidence: 91%

Section: Effect Of Liposome Injection On the Depletion Of Ayu Monocytmentioning

confidence: 99%

LECT2 improves the outcomes in ayu with Vibrio anguillarum infection via monocytes/macrophages

Chen

et al. 2014

Fish & Shellfish Immunology

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“…Antibodies were added according to the manufacturer's protocols. Goldfish leukocytes were stained with a polyclonal anti-CSF-1R antibody that was produced and validated by the Belosevic lab (University of Alberta) [35]. Cells were stained with 1x PBS −/− with 2% calf serum and 0.05% sodium azide.…”

Section: Methodsmentioning

confidence: 99%

Fish and Mammalian Phagocytes Differentially Regulate Pro-Inflammatory and Homeostatic Responses In Vivo

et al. 2012

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Phagocytosis is a cellular mechanism that is important to the early induction of antimicrobial responses and the regulation of adaptive immunity. At an inflammatory site, phagocytes serve as central regulators for both pro-inflammatory and homeostatic anti-inflammatory processes. However, it remains unclear if this is a recent evolutionary development or whether the capacity to balance between these two seemingly contradictory processes is a feature already displayed in lower vertebrates. In this study, we used murine (C57BL/6) and teleost fish (C. auratus) in vitro and in vivo models to assess the evolutionary conservation of this dichotomy at a site of inflammation. At the level of the macrophage, we found that teleost fish already displayed divergent pro-inflammatory and homeostatic responses following internalization of zymosan or apoptotic bodies, respectively, and that these were consistent with those of mice. However, fish and mice displayed significant differences in vivo with regards to the level of responsiveness to zymosan and apoptotic bodies, the identity of infiltrating leukocytes, their rate of infiltration, and the kinetics and strength of resulting antimicrobial responses. Unlike macrophages, significant differences were identified between teleost and murine neutrophilic responses. We report for the first time that activated murine, but not teleost neutrophils, possess the capacity to internalize apoptotic bodies. This internalization translates into reduction of neutrophil ROS production. This may play an important part in the recently identified anti-inflammatory activity that mammalian neutrophils display during the resolution phase of inflammation. Our observations are consistent with continued honing of inflammatory control mechanisms from fish to mammals, and provide added insights into the evolutionary path that has resulted in the integrated, multilayered responses that are characteristic of higher vertebrates.

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“…Zebrafish raised at cooler temperatures (17°C versus 26.5°C) for up to 7 months display a selective decrease in erythrocytes, but not myeloid cells, resulting in an anemic state [ 49 ]. Examination of the gene expression of key growth factors involved in hematopoiesis revealed a decrease in the expression of erythropoietin ( epo ) and erythropoietin receptor ( epor) in kidney marrow, important for erythropoiesis [ 50 , 51 , 52 ], but not in colony-stimulating factor-1a ( csf1a) or csf3 expressions that are important for generation of macrophages and granulocytes, respectively [ 49 , 53 , 54 , 55 ]. These studies suggest that while macrophages and granulocytes (neutrophils) are still produced at normal levels, the expressions of key proinflammatory cytokines and proteins involved in viral recognition may be suppressed or delayed, at least at the transcript level, under hypothermic levels during fish ontogeny.…”

Section: Innate Immunity and Temperaturementioning

confidence: 99%

Impacts of Low Temperature on the Teleost Immune System

Abram

Dixon

Katzenback

2017

Biology

130

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As poikilothermic vertebrates, fish can experience changes in water temperature, and hence body temperature, as a result of seasonal changes, migration, or efflux of large quantities of effluent into a body of water. Temperature shifts outside of the optimal temperature range for an individual fish species can have negative impacts on the physiology of the animal, including the immune system. As a result, acute or chronic exposure to suboptimal temperatures can impair an organisms’ ability to defend against pathogens and thus compromise the overall health of the animal. This review focuses on the advances made towards understanding the impacts of suboptimal temperature on the soluble and cellular mediators of the innate and adaptive immune systems of fishes. Although cold stress can result in varying effects in different fish species, acute and chronic suboptimal temperature exposure generally yield suppressive effects, particularly on adaptive immunity. Knowledge of the effects of environmental temperature on fish species is critical for both the optimal management of wild species and the best management practices for aquaculture species.

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Colony-stimulating factor-1 receptor protein expression is a specific marker for goldfish (Carassius auratus L.) macrophage progenitors and their differentiated cell types

Cited by 26 publications

References 40 publications

LECT2 improves the outcomes in ayu with Vibrio anguillarum infection via monocytes/macrophages

LECT2 improves the outcomes in ayu with Vibrio anguillarum infection via monocytes/macrophages

Fish and Mammalian Phagocytes Differentially Regulate Pro-Inflammatory and Homeostatic Responses In Vivo

Impacts of Low Temperature on the Teleost Immune System

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