Colonoscopy in rats: An endoscopic, histological and tomographic study

Bartolı́, Ramón; Boix, Jaume; Òdena, Gemma; Ossa, Napoleón D De la; Vega, Vicente Moreno de; Lorenzo-Zúñiga, Vicente

doi:10.4253/wjge.v5.i5.226

Cited by 11 publications

(9 citation statements)

References 22 publications

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“…Conventional EMR technique with prior submucosal injection was not able to be done as a result of wall thickness . Lesions were created in the left colon, 6 cm to the anal margin, by Coagrasper Hemostatic Forceps (Olympus), with a power setting of forced coagulation 40 W during 4 s .…”

Section: Methodsmentioning

confidence: 99%

Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Lorenzo-Zúñiga

Boix

Vega

et al. 2017

Digestive Endoscopy

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Section: Methodsmentioning

confidence: 99%

Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Lorenzo-Zúñiga

Boix

Vega

et al. 2017

Digestive Endoscopy

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“…Colonoscopy plays a fundamental role in the diagnosis and the monitoring of disease activity [ 60 ]. As presented study showed, FMT was able to alleviate symptoms of UC, such as thickening of the intestinal wall, bleeding and presence of fibrin fibres in the intestinal lumen.…”

Section: Discussionmentioning

confidence: 99%

Single Donor FMT Reverses Microbial/Immune Dysbiosis and Induces Clinical Remission in a Rat Model of Acute Colitis

et al. 2021

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Deviation in the gut microbial composition is involved in various pathologies, including inflammatory bowel disease (IBD). Faecal microbiota transplant (FMT) can act as a promising approach to treat IBD by which changes in microbiome can be reversed and homeostasis restored. Therefore, the aim of this study was to investigate the effect of FMT on the remission of acute inflammatory response using dextran sulfate sodium (DSS)-induced rat colitis model. Faecal microbial communities were analysed using the 16S rRNA approach, and clinical manifestations together with histological/haematological/biochemical/immunological analyses were assessed. Our study demonstrated significant shifts in the dominant species of microbiota under inflammatory conditions induced by DSS and evident restoration effect of FMT treatment on microbial composition. These faecal microbial alterations in FMT-treated rats led to a relative restoration of colon length, and a significant decrease in both epithelium damage and disease severity, which was reflected in lower serum pro-inflammatory cytokine levels. Haematological/biochemical parameters in DSS-treated animals showed signs of anaemia with a significant reduction in red blood cell count together with increasing levels of total bilirubin, creatinine and phosphorus suggesting potential protective effect of FMT. These results support FMT as a valuable therapeutic strategy to control inflammation during acute colitis.

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“…We also evaluated the feasibility of endoscopy and biopsy to assess for the presence of inflammation and/or the changes of metaplasia in a longitudinal model without animal sacrifice. The use of colonoscopic animal models have been developed and in some cases, coupled with radiographic measures 20, 21 to study chemically-induced colitis 22–25 and colorectal tumors. 26 To our knowledge, using endoscopy to study a surgical model of IRA or IPAA has not been previously performed.…”

Section: Discussionmentioning

confidence: 99%

Development of a total colectomy and ileorectal anastomosis rat model to evaluate colonic metaplasia

Schieffer

Poritz

Yochum

et al. 2017

Journal of Surgical Research

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Background Ulcerative colitis (UC) is an idiopathic inflammatory condition of the colon that may require surgical intervention including proctocolectomy and either ileal pouch-anal anastomosis (IPAA) or in the pediatric population, low ileorectal anastomosis (IRA). Often, subsequent physiologic alteration (or colonic metaplasia) occurs in the anastomosed small bowel that includes changes in mucin content, villous blunting, and increased expression of WNT5A, a marker of colonic crypt regeneration. We developed a rat low IRA model to assess and study the development of colonic metaplasia. Materials and methods We subjected male Sprague-Dawley rats (n=17) to total colectomy and low IRA surgery and evaluated healing periodically by endoscopic evaluation. The ileum upstream of the anastomosis was assessed by hematoxylin and eosin (H&E) staining and the mucin content was measured by high iron diamine-Alcian blue (HID-AB) staining. Wnt5a transcripts were quantified by RT-qPCR at the 8 week study endpoint. Results Although no gross endoscopic evidence of inflammation was seen throughout the course of the study, colonic metaplasia in the small bowel was detected in 7 out of 10 (70%) rats at the study endpoint. In rats with colonic metaplasia, enhanced expression of Wnt5a was evident at the study endpoint compared to levels in the terminal ileum at the time of surgery. Conclusions Within 4–8 weeks, the majority of rats subjected to IRA developed colonic metaplasia defined by villous blunting, changes in mucin content, and increased expression of Wnt5a. This model provides a method to study small bowel colonic metaplasia.

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Colonoscopy in rats: An endoscopic, histological and tomographic study

Abstract: The combination of colonoscopy, tomography and histology leads to a better characterization of the entire colon. These data are important for deciding when to perform endoscopic resections or when to induce perforations to apply endoscopic treatments.

Cited by 11 publications

References 22 publications

Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Single Donor FMT Reverses Microbial/Immune Dysbiosis and Induces Clinical Remission in a Rat Model of Acute Colitis

Development of a total colectomy and ileorectal anastomosis rat model to evaluate colonic metaplasia

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