Colonization status and community structure of arbuscular mycorrhizal fungi in the coniferous tree, Cryptomeria japonica, with special reference to root orders

Matsuda, Yosuke; Kita, K; Kitagami, Yudai; Tanikawa, Toko

doi:10.1007/s11104-021-05147-w

Cited by 19 publications

(13 citation statements)

References 66 publications

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“…This discrepancy can be explained by the use of different hosts, sites, seasons, AMF quantification proxies, and overall approaches [ 2 , 4 , 8 ], which varied among prior studies but were controlled in this work. In previous studies of the AMF communities of Cj and Co [ 19 , 20 , 38 ], root and soil OTU richness were not both evaluated, thus precluding comparison of intra- and extraradical AMF communities. In Venn diagrams, the number of AMF OTUs exclusive to the roots of Cj or Co decreased when data from all sites were considered (Online Resource 2 ).…”

Section: Discussionmentioning

confidence: 99%

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Paired Root-Soil Samples and Metabarcoding Reveal Taxon-Based Colonization Strategies in Arbuscular Mycorrhizal Fungi Communities in Japanese Cedar and Cypress Stands

2023

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Arbuscular mycorrhizal fungi (AMF) in the roots and soil surrounding their hosts are typically independently investigated and little is known of the relationships between the communities of the two compartments. We simultaneously collected root and surrounding soil samples from Cryptomeria japonica (Cj) and Chamaecyparis obtusa (Co) at three environmentally different sites. Based on molecular and morphological analyses, we characterized their associated AMF communities. Cj was more densely colonized than Co and that root colonization intensity was significantly correlated with soil AMF diversity. The communities comprised 15 AMF genera dominated by Glomus and Paraglomus and 1443 operational taxonomic units (OTUs) of which 1067 and 1170 were in roots and soil, respectively. AMF communities were significantly different among sites, and the root AMF communities were significantly different from those of soil at each site. The root and soil AMF communities responded differently to soil pH. At the genus level, Glomus and Acaulospora were abundant in roots while Paraglomus and Redeckera were abundant in soil. Our findings suggest that AMF colonizing roots are protected from environmental stresses in soil. However, the root-soil-abundant taxa have adapted to both environments and represent a model AMF symbiont. This evidence of strategic exploitation of the rhizosphere by AMF supports prior hypotheses and provides insights into community ecology.

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Section: Discussionmentioning

confidence: 99%

“…However, no study has assessed Co root colonization. Furthermore, few studies such as those by Zou et al [ 19 ], Matsuda et al [ 20 ], and Djotan et al [ 10 ] have investigated the AMF communities associated with Cj. To our knowledge, no study has compared the AMF community of the roots and surrounding soil of Cj and Co.…”

Section: Introductionmentioning

confidence: 99%

Paired Root-Soil Samples and Metabarcoding Reveal Taxon-Based Colonization Strategies in Arbuscular Mycorrhizal Fungi Communities in Japanese Cedar and Cypress Stands

2023

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“…According to the MaarjAM, the VTX166 and VTX159 have been detected in various host plants from liverworts to seed plants on six continents. It has also been reported that VTX166 corresponded to one of the dominant OTUs in Japanese temperate forests (Miyake, Ishitsuka, Taniguchi, & Yamato, 2020;Matsuda, Kita, Kitagami, & Tanikawa, 2021). It was suggested that mycoheterotrophic plants preferentially target AM fungi that are well connected to many surrounding autotrophic plants (Gomes et al, 2022).…”

Section: Discussionmentioning

confidence: 90%

Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings of <i>Gentiana zollingeri</i> (<i>Gentianaceae</i>)

Kusakabe

Yamato

2023

Mycoscience

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Gentiana zollingeri (Gentianaceae) is an initial mycoheterotrophic plant that depends on a specific group of arbuscular mycorrhizal (AM) fungi for carbon source during underground growth after seed germination. In this study, a mycorrhizal fungus dominant in mycoheterotrophic seedlings of G. zollingeri was successfully isolated from a soil core collected from a point close to a flowering G. zollingeri. The AM fungal isolate was identified as conspecific or closely related to Dominikia aurea (Glomeraceae) by spore morphology and molecular phylogeny. Basic Local Alignment Search Tool (BLAST) searches against the MaarjAM database showed that the nuclear small subunit ribosomal DNA sequences of the isolate matched the AM fungal sequences obtained from a wide range of plants in various ecosystems, including several mycoheterotrophs. Thus, it is suggested that the AM fungal isolate is one of the cheating susceptible AM fungi. Furthermore, the sequences corresponded to those of a group of AM fungi dominantly detected in Japanese temperate forests. Accordingly, there is a possibility that mycoheterotrophic plants, including seedlings of G. zollingeri, may target AM fungi with a wide host range and ubiquitous distribution.

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“…Genomes of some other types of ascoand basidiomycetous pathogens, such as some on leaves (Dhillon et al 2015;Duplessis et al 2011a;Zhu et al 2012) and of wilt-, blight-and cancer-pathogens in bark and xylem are available, including those of several insect-associated fungal species (Alamouti et al 2011;Comeau et al 2015;Crouch et al 2020;Demené et al 2022;Dhillon et al 2015;Ibarra Caballero et al 2019;Sbaraini et al 2017;Schuelke et al 2017;Stauber et al 2020;Stenlid et al 2017;Yin et al 2015). There is also a growing collection of genomes of symbiotic ECM species (e.g., Kohler et al 2015;Lofgren et al 2021;Looney et al 2022;Martin et al 2008Martin et al , 2010Martino et al 2018;Miyauchi et al 2020b;Peter et al 2016;Wagner et al 2015), some ericoid mycorrhizal ascomycetous fungi (Kohler et al 2015;Martino et al 2018;Perotto et al 2018), an arbuscular mycorrhizal fungus from the conifer Cryptomeria japonica (Matsuda et al 2021), and of a few tree endophytes (Gazis et al 2016;Knapp et al 2018;Schlegel et al 2016) while plant litter decay fungi and in general saprotrophic soil fungi have so far rather been neglected (Barbi et al 2020).…”

Section: Fungal Genomes and Transcriptomesmentioning

confidence: 99%

Applying molecular and genetic methods to trees and their fungal communities

Müller

Kües

Budde

et al. 2023

Appl Microbiol Biotechnol

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Forests provide invaluable economic, ecological, and social services. At the same time, they are exposed to several threats, such as fragmentation, changing climatic conditions, or increasingly destructive pests and pathogens. Trees, the inherent species of forests, cannot be viewed as isolated organisms. Manifold (micro)organisms are associated with trees playing a pivotal role in forest ecosystems. Of these organisms, fungi may have the greatest impact on the life of trees. A multitude of molecular and genetic methods are now available to investigate tree species and their associated organisms. Due to their smaller genome sizes compared to tree species, whole genomes of different fungi are routinely compared. Such studies have only recently started in forest tree species. Here, we summarize the application of molecular and genetic methods in forest conservation genetics, tree breeding, and association genetics as well as for the investigation of fungal communities and their interrelated ecological functions. These techniques provide valuable insights into the molecular basis of adaptive traits, the impacts of forest management, and changing environmental conditions on tree species and fungal communities and can enhance tree-breeding cycles due to reduced time for field testing. It becomes clear that there are multifaceted interactions among microbial species as well as between these organisms and trees. We demonstrate the versatility of the different approaches based on case studies on trees and fungi. Key points • Current knowledge of genetic methods applied to forest trees and associated fungi. • Genomic methods are essential in conservation, breeding, management, and research. • Important role of phytobiomes for trees and their ecosystems.

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Colonization status and community structure of arbuscular mycorrhizal fungi in the coniferous tree, Cryptomeria japonica, with special reference to root orders

Cited by 19 publications

References 66 publications

Paired Root-Soil Samples and Metabarcoding Reveal Taxon-Based Colonization Strategies in Arbuscular Mycorrhizal Fungi Communities in Japanese Cedar and Cypress Stands

Paired Root-Soil Samples and Metabarcoding Reveal Taxon-Based Colonization Strategies in Arbuscular Mycorrhizal Fungi Communities in Japanese Cedar and Cypress Stands

Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings of <i>Gentiana zollingeri</i> (<i>Gentianaceae</i>)

Applying molecular and genetic methods to trees and their fungal communities

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