Colonization potential to reconstitute a microbe community in patients detected early after fecal microbe transplant for recurrent C. difficile

Kumar, Ranjit; Maynard, Craig L.; Eipers, Peter; Goldsmith, Kelly T.; Ptacek, Travis; Grubbs, James; Dixon, Paula; Howard, Donna; Crossman, David K.; Crowley, Michael R.; Benjamin, William H.; Lefkowitz, Elliot J.; Weaver, Casey T.; Rodríguez, Jorge Martin; Morrow, Casey D.

doi:10.1186/s12866-015-0622-2

Cited by 21 publications

(26 citation statements)

References 44 publications

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“…This study provides additional evidence linking the human intestinal microbiota with the pathogenesis of arthritis. We did not demonstrate differences in the extent of arthritis between mice colonized with patient versus control microbiota, findings that could be attributed to the failure of human-mouse FMT to recapitulate the precise microbial contents of the human donors, as observed herein ( Figure 3 ) and previously [ 11 , 12 ]. Our prior work demonstrated that a subset of children with ERA demonstrated a high intestinal abundance of A. muciniphila [ 1 ].…”

Section: Discussionsupporting

confidence: 72%

Akkermansia muciniphila is permissive to arthritis in the K/BxN mouse model of arthritis

et al. 2018

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Studies have identified abnormalities in the microbiota of patients with arthritis. To evaluate the pathogenicity of human microbiota, we performed fecal microbial transplantation from children with spondyloarthritis and controls to germ-free KRN/B6xNOD mice. Ankle swelling was equivalent in those that received patient vs control microbiota. Principal coordinates analysis revealed incomplete uptake of the human microbiota with over-representation of two genera (Bacteroides and Akkermansia) among the transplanted mice. The microbiota predicted the extent of ankle swelling (R2 = 0.185, p = 0.018). The abundances of Bacteroides (r = −0.510, p = 0.010) inversely and Akkermansia.(r = 0.367, p = 0.078) directly correlated with ankle swelling. Addition of A. muciniphila to Altered Schaedler’s Flora (ASF) resulted in small but statistically significant increased ankle swelling as compared to mice that received ASF alone (4.0 mm, 3.9 – 4.1 versus 3.9 mm, IQR 3.6 – 4.0, p = 0.041), as did addition of A. muciniphila cultures to transplanted human microbiota as compared to mice that received transplanted human microbiota alone (4.5 mm, IQR 4.3 – 5.5 versus 4.1 mm, IQR 3.9 – 4.3, p = 0.019). This study supports previous findings of an association between Akkermansia muciniphila and arthritis.

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Section: Discussionsupporting

confidence: 72%

Akkermansia muciniphila is permissive to arthritis in the K/BxN mouse model of arthritis

et al. 2018

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“…Mice were housed by human sample chemotherapy status and diet [ Table 1 ] (4 mice/ cage- a total 48 mice in 12 cages). Fecal suspension (200 μl) was transplanted into mice using oral-gavage and enema routes [ 35 ]. Co-housing of four GF mice with one colonized mouse led to successful colonization of all four GF mice.…”

Section: Methodsmentioning

confidence: 99%

Impact of genistein on the gut microbiome of humanized mice and its role in breast tumor inhibition

Paul

Royston

et al. 2017

PLoS ONE

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Since dietary polyphenols can have beneficial effects in prevention and treatment of cancer, we tested the hypothesis that breast cancer patients’ intestinal microbiota is modulated by genistein (GE), an isoflavone found in soy, and that microbial alterations may offset the side effects brought about by chemotherapy. We demonstrated successful humanization of germ-free mice by transplanting fecal samples from breast cancer patients before and after chemotherapy. Mice were then grouped based on chemotherapy status and GE or control diet. We did not find any significant differences between pre-chemotherapy and post-chemotherapy bacterial composition and abundances. Germ-free mice on a GE diet showed differences in microbial composition as compared to mice on control diet. Four weeks after introduction of the customized GE diet, there was distinct clustering of GE-fed mice as compared to the control-fed group. In the gut microbiome of GE-treated humanized mice, there was an increase in abundance of genera Lactococcus and Eubacterium. Phylum Verrucomicrobia showed statistically significant (p = 0.02) differences in abundances between the GE-fed and control-fed groups. There was an increase in bacteria belonging to family Lachnospiraceae and Ruminococcaceae in GE-fed mice. Marked changes were observed in GE catabolism in mice humanized with fecal material from two of three patients’ post-chemotherapy with complete disappearance of 4-ethylphenol and 2-(4-hydroxyphenol) propionic acid conjugates. The post-tumor samples did not show any distinct clustering of the gut microbiota between the two diet groups. There was an increase in latency of about 25% for tumor growth of the humanized mice that were on a GE diet as compared to humanized mice on a control diet. The average tumor size for the GE group was significantly decreased compared to the non-GE group. Collectively, our results suggest that the intestinal microbiota becomes altered with a GE diet before induction of tumor. Our findings indicate that GE modulates the microbiome in humanized mice that may contribute to its effects on increasing the latency of breast tumor and reducing tumor growth.

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“…Mice were individually housed in separate isolators in the UAB Gnotobiotic Facility, and sterile (autoclaved) mouse chow (Teklad #2019S) was supplied ad libitum. Previously, we determined that two weeks post-transplant was sufficient to allow re-constitution of an intact microbiome [22].…”

Section: Faecal Transplantation Of Gnotobiotic Micementioning

confidence: 99%

“…Faecal samples from individual mice were processed for DNA as previously described [22]. The DNA from a total of 30 faecal samples (5 human faeces, 5 dam faeces and 20 F1 progeny faeces) was prepared, processed and sequenced using the Illumina NextSeq platform, with 150 base paired-end reads with an average of 17 million reads per sample (electronic supplementary material, table S1).…”

Section: Metagenomic Sequencing Of Murine Faecal Samplesmentioning

confidence: 99%

An individualized mosaic of maternal microbial strains is transmitted to the infant gut microbial community

et al. 2020

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To understand the origins of the infant gut microbial community, we have used a published metagenomic dataset of the faecal microbiome of mothers and their related infants at early (4, 7 and 21 days) and late times (6–15 months) following birth. Using strain-tracking analysis, individual-specific patterns of microbial strain sharing were found between mothers and infants following vaginal birth. Overall, three mother–infant pairs showed only related strains, while 12 infants of mother–infant pairs contained a mosaic of maternal-related and unrelated microbes. Analysis of a second dataset from nine women taken at different times of pregnancy revealed individual-specific faecal microbial strain variation that occurred in seven women. To model transmission in the absence of environmental microbes, we analysed the microbial strain transmission to F1 progenies of human faecal transplanted gnotobiotic mice bred with gnotobiotic males. Strain-tracking analysis of five different dams and their F1 progeny revealed both related and unrelated microbial strains in the mother's faeces. The results of our analysis demonstrate that multiple strains of maternal microbes, some that are not abundant in the maternal faecal community, can be transmitted during birth to establish a diverse infant gut microbial community.

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Colonization potential to reconstitute a microbe community in patients detected early after fecal microbe transplant for recurrent C. difficile

Cited by 21 publications

References 44 publications

Akkermansia muciniphila is permissive to arthritis in the K/BxN mouse model of arthritis

Akkermansia muciniphila is permissive to arthritis in the K/BxN mouse model of arthritis

Impact of genistein on the gut microbiome of humanized mice and its role in breast tumor inhibition

An individualized mosaic of maternal microbial strains is transmitted to the infant gut microbial community

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