Colonization Patterns of an mCherry-Tagged <i>Pectobacterium carotovorum</i> subsp. <i>brasiliense</i> Strain in Potato Plants

Kubheka, Gugulethu Cresentia; Coutinho, Teresa A.; Moleleki, Ntsane; Moleleki, Lucy N.

doi:10.1094/phyto-02-13-0049-r

Cited by 43 publications

(29 citation statements)

References 38 publications

(54 reference statements)

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“…Trace amounts of Pcb_mCherry were isolated from roots of both treated and untreated plants inoculated with Pcb_mCherry (Fig 2). This confirmed our previous observations that Pcb has a limited ability to infect intact potato plant roots and tubers Kubheka et al, 2013).…”

Section: The Effect Of Three Elicitors On Rkn Reproduction I Potato Psupporting

confidence: 92%

The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

Mongae

Moleleki

2015

Eur J Plant Pathol

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The efficacy of three elicitors, DL-β-aminobutyric acid (BABA), Acibenzolar-S-methyl (Bion) and Messenger (Harpin) on potato plants inoculated with Meloidogyne incognita second stage juveniles (J2s) was investigated in this study. The efficacy of each elicitor was evaluated based on galling index, egg numbers, total root knot nematode (RKN) population numbers, number of juveniles and the percentage of females in respective extracted populations per treatment at 28 and 56 dpi. There was a significant decrease in galling indices of all treated compared to untreated control plants. The highest percentage reduction was observed for 20 mM BABA-treated plants (33%) followed by 5mM BABA (23%), 50mg/L Bion (18%), 100mg/L Bion (15%) and Messenger (8%). The number of eggs extracted from plants treated with 20mM BABA was significantly lower (66%) compared to untreated controls. Furthermore, treating plants with 20mM BABA decreased the total RKN population numbers by 55% whilst the decrease observed for 2 Bion and Messenger was between 24-39%. Of the three tested elicitors, 20mM BABA was found to be the most effective elicitor at both 28 and 56 dpi. Hence, it was selected for further testing against RKN and Pectobacterium carotovorum subsp. brasiliense (Pcb) disease complex. Efficacy was tested by comparing the colony forming units (cfu) of Pcb from treated and untreated control plants inoculated with M. incongita and Pcb simultaneously. The concentration of Pcb from untreated plants inoculated with both pathogens was significantly higher (3.3 × 10 3 cfu/ml) compared to their treated counterparts (8.71 × 10 2 cfu/ml). This suggests that BABA has the potential to decrease soft rot bacterial infections in RKN disease complexes by decreasing the rate of RKN infection. This is the first report where BABA is shown to decrease the infection rate of RKN as a primary causal agent in combination with a secondary pathogen.

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Section: The Effect Of Three Elicitors On Rkn Reproduction I Potato Psupporting

confidence: 92%

The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

Mongae

Moleleki

2015

Eur J Plant Pathol

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“…brasiliense colonizes the xylem tissue of symptomatic potatoes and forms biofilm-like aggregates that increase its capacity to remain in the infected host21. Here, we assessed the effect of the phenolic volatiles carvacrol and eugenol on the biofilm formation ability of two Pectobacterium strains, P. carotovorum subsp .…”

Section: Resultsmentioning

confidence: 99%

Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins

Joshi

Khazanov

Senderowitz

et al. 2016

Sci Rep

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Quorum sensing (QS) is a population density-dependent regulatory system in bacteria that couples gene expression to cell density through accumulation of diffusible signaling molecules. Pectobacteria are causal agents of soft rot disease in a range of economically important crops. They rely on QS to coordinate their main virulence factor, production of plant cell wall degrading enzymes (PCWDEs). Plants have evolved an array of antimicrobial compounds to anticipate and cope with pathogens, of which essential oils (EOs) are widely recognized. Here, volatile EOs, carvacrol and eugenol, were shown to specifically interfere with QS, the master regulator of virulence in pectobacteria, resulting in strong inhibition of QS genes, biofilm formation and PCWDEs, thereby leading to impaired infection. Accumulation of the signal molecule N-acylhomoserine lactone declined upon treatment with EOs, suggesting direct interaction of EOs with either homoserine lactone synthase (ExpI) or with the regulatory protein (ExpR). Homology models of both proteins were constructed and docking simulations were performed to test the above hypotheses. The resulting binding modes and docking scores of carvacrol and eugenol support potential binding to ExpI/ExpR, with stronger interactions than previously known inhibitors of both proteins. The results demonstrate the potential involvement of phytochemicals in the control of Pectobacterium.

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“…Pectobacterium species are usually found in tuber lenticels, on roots or colonizing and occluding potato plant xylem (Charkowski, 2015). Previous work on the host-pathogen interaction between potato and soft rot bacterial pathogens has mainly focused on the pathogen, that is, its pathogenicity and colonization patterns of tubers, roots and stems (Czajkowski et al, 2010; Kubheka et al, 2013). However, not much has been reported on the host potato stem responses against soft rot pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…BP1, susceptible and tolerant to P. carotovorum subsp brasiliense strain 1692 ( Pcb1692 ) infection, respectively, were greenhouse grown under standard conditions (22 to 26°C, 16 h light/8 h dark photoperiod and 70% relative humidity). Stem inoculations were done following the approach previously described in (Kubheka et al, 2013), except that in this study we only used wild-type P. carotovorum subsp. brasiliense 1692 for the inoculations.…”

Section: Methodsmentioning

confidence: 99%

“…We previously reported on a potato cultivar S. tuberosum cv BP1 that shows significant tolerance to P. c. brasilense strain 1692 (Pcb1692) compared to the more susceptible S. tuberosum cv Valor (Kubheka et al, 2013). Thus, we wanted to use this tolerant vs susceptible model to further dissect the molecular basis of tolerance in S. tuberosum cv BP1, particularly in the early stages of infection (0–72 hpi) that signify the transition from asymptomatic to symptomatic phase in Pcb1692 within the susceptible cultivar.…”

Section: Introductionmentioning

confidence: 99%

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RNA-seq Profiling Reveals Defense Responses in a Tolerant Potato Cultivar to Stem Infection by Pectobacterium carotovorum ssp. brasiliense

et al. 2016

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Pectobacterium carotovorum subsp. brasiliense is a member of the soft rot Enterobacteriaceae (SRE) family that causes tuber soft rot and blackleg diseases of stems in potato plants. Currently, there are no effective chemical strategies for the control of members of the SRE. Thus, an understanding of the inducible defense responses in stems of potato plants is important, particularly during colonization of the vascular system. Here, time-course RNA-sequencing analysis was used to compare expressed genes between a susceptible potato cultivar (Solanum tuberosum cv Valor) and a tolerant cultivar (S. tuberosum cv BP1) at 0, 6, 12, 24, and 72 h post-inoculation with P. c. brasiliense. In total, we identified 6139 and 8214 differentially expressed genes (DEGs) in the tolerant and susceptible cultivars, compared to mock-inoculated controls, respectively. Key DEGs distinguishing between tolerance and susceptibility were associated with negative regulation of cell death and plant-type cell wall organization/biogenesis biological processes in the tolerant and susceptible cultivars, respectively. Among these were DEGs involved in signaling (mainly MAPK cascade and ethylene pathway), defense-related transcription regulation including WRKY transcription factors, and downstream secondary cell biosynthesis. Together, our results suggest that S. tuberosum cv BP1 likely employs quantitative defense response against P. c. brasiliense. Overall, our study provides the first transcriptome-wide insight into the molecular basis of tolerance and/or resistance of potato stems to SRE infection.

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Colonization Patterns of an mCherry-Tagged Pectobacterium carotovorum subsp. brasiliense Strain in Potato Plants

Cited by 43 publications

References 38 publications

The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins

RNA-seq Profiling Reveals Defense Responses in a Tolerant Potato Cultivar to Stem Infection by Pectobacterium carotovorum ssp. brasiliense

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