Colonization patterns and topochemical aspects of sap streak caused by Stereum sanguinolentum in Norway spruce

Abstract

Summary Fresh sapwood blocks of Norway spruce (Picea abies) were exposed to 12‐ and 60‐day attack by Stereum sanguinolentum in pure culture and were examined with respect to structural, topochemical and colour alterations. Final stages of degradation by 5. sanguinolentum were studied on naturally decayed spruce wood after 1 year of fungal growth. The development of discoloration in the main anatomical directions of wood was assessed. Furthermore, microspectrophotometrical studies on the decomposition of cell wall polyphenols were performed. Initial signs of sap streak appeared already 8 days after fungal infection. Within the three‐dimensional decay pattern, mycelial aggregations associ‐ated with the ray tissue proved to be the major cause of discoloration. UV extinction measurements taken at 280 nm of different parts of cell wall transverse sections revealed a specific pattern of lignin distribution and its derivatives during fungal degradation.

Application of Scanning UV Microspectrophotometry to Localise Lignins and Phenolic Extractives in Plant Cell Walls

“…Fukazawa and Imagawa 1981;Takabe et al 1992), (ii) the deterioration of lignin during fungal wood decay (e.g. Bauch et al 1976;Kleist and Seehann 1997;Kleist and Schmitt 2001), (iii) the characterisation of acetylated and hydrothermally treated tissues (Sander and Koch 2001), (iv) the localisation and analyses of cell wall wood extractives (e.g. Bland and Hillis 1969;Hillis et al 1976;Imagawa and Fukazawa 1978;Kleist and Bauch 2001) and (v) the chemical composition of pit membranes of gymnosperms (e.g.…”

Permeability changes in heartwood of Picea abies and Abies alba induced by incubation with Physisporinus vitreus

“…The latter orientation was used for the smaller specimens, to allow them to rest on their broader (radial) surfaces. The radial surfaces of wood specimens are relatively resistant to colonisation by fungal hyphae (Kleist and Seehann 1997;Schwarze et al 2004). Thus, a more rapid hyphal growth in the radial than tangential direction resulted in a higher increase of water uptake in larger wood specimens irrespective of the wood species.…”

Effect of Physisporinus vitreus on wood properties of Norway spruce. Part 1: Aspects of delignification and surface hardness

“…Bauch et al (1976) applied UMSP successfully to investigate topochemical delignification caused by fungal decay in woody tissue. Kleist and Seehann (1997) described effects of fungal activity in latewood regions of Norway spruce wood after 8 weeks incubation with white rot fungus Stereum sanguinolentum. Lower lignin contents caused by fungal delignification result in lower absorbances at 280 nm.…”