Colonization of the rabbit small intestine by clinical and environmental isolates of non-O1 Vibrio cholerae and Vibrio mimicus

Spira, William M.; Fedorka-Cray, Paula J.; Pettebone, P

doi:10.1128/iai.41.3.1175-1183.1983

Cited by 30 publications

(19 citation statements)

References 21 publications

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“…Therefore, in contrast to 987P and K99 fimbriae, TCP and BFP expression is expected to be activated at an earlier stage of infection. Consistent with this, colonization by V. cholerae (Nelson et al, 1976;Cray et al, 1983;Spira et al, 1983;Pierce et al, 1985) and EPEC (Ulshen and Rollo, 1980;Rothbaum et al, 1982; has been described to involve proximal as well as distal segments of the small intestine.…”

Section: Discussionmentioning

confidence: 52%

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Edwards

Schifferli

1997

Molecular Microbiology

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SummaryAn early process in the pathogenesis of enteric bacteria is colonization of the intestinal epithelium leading to local multiplication, pathophysiological interactions with the host and further spreading. Attachment is typically mediated by bacterial fimbriae, which are selectively expressed during growth in the intestine. Here we report an analysis of the regulation of 987P fimbrial expression of enterotoxigenic Escherichia coli (ETEC). Expression of both fasH, the transcriptional activator of the 987P fimbrial genes, and fasA, the major fimbrial subunit, is regulated in response to a variety of environmental stimuli. We have found that expression of fasH is regulated in response to the carbon status of the growth medium by the cAMP-CRP complex. Moreover, fasH is regulated in response to both the nitrogen status of the growth medium and the external pH. Expression of fasA is activated by FasH, and is also selectively regulated in response to growth temperature by HNS. Regulation of fimbrial expression by carbon and/or nitrogen gradients is proposed to provide a mechanism that allows preferential colonization of different segments of the intestine by various enteropathogens, such as ETEC, enteropathogenic E. coli and Vibrio cholerae.

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Section: Discussionmentioning

confidence: 52%

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Edwards

Schifferli

1997

Molecular Microbiology

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“…It is also noteworthy that this bacterium is capable of producing haemolysins, a group of toxins considered to constitute a common pathogenic factor shared by various species of the genus Vibrio [19]. In addition, Spira et al [20] reported that the majority of isolates from diarrhoeal stools displayed this characteristic, and the Table 2 Results assay with non-01 V cholerae in animal model (permeability factor and fluid accumulation) same characteristic was observed in 89.7% of isolates taken from the environment [1]. In this study, haemolysin was detected in 63.28% of the samples.…”

Section: Resultsmentioning

confidence: 99%

Analysis of some factors involved in the virulence mechanism of Vibrio cholerae non-01

Rodrigues¹

1993

FEMS Immunology and Medical Microbiology

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A study was carried out to evaluate the potential intestinal toxicity of 188 samples of Vibrio cholerae non-01 isolated from seawater found along the beaches of Rio de Janeiro city. Three different assays were carried out involving: (a) detection of vascular permeability factor (PF) in guinea pigs (together with assessment of two culture media for production of the toxin); (b) intestinal fluid accumulation (FA) in suckling mice; and (c) detection of haemolysin. The results demonstrated that both culture media gave a similar level of performance. In the animal assays, 43% of the samples induced PF in guinea pigs, 28.7% caused intestinal fluid accumulation in suckling mice, and 63.28% contained haemolysin. Only 4.25% of the samples gave positive results in all three tests.

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“…Colonization of rabbit small intestinal mucosa by clinical and environmental isolates of V. cholerae and V. mimicus was shown (25). Correlation has been established between in vitro adherence to intestinal epithelium of human (26) and rabbit (14) and the cell-associated haemagglutination of V. cholerae.…”

Section: Activation Of Cell-associated Vm-omphamentioning

confidence: 94%

“…Correlation between the adherence and the cellmediated haemagglutination has been established for many intestinal pathogens (10,18,25,26), and cell surface proteins, such as haemagglutinins (HAs) and/or different types of pili, presumably accomplish both bacterial phenomena (20). In Vibrio cholerae, the etiologic agent of cholera, the toxin-coregulated pilus (TCP) has been documented to be essential for adherence of the classical biotype strains (13); however, the mannosesensitive HA as well as TCP has been implicated in the case of the El Tor strains (21).…”

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confidence: 99%

Proteolytic Activation of Vibrio mimicus (Vm) Major Outer Membrane Protein Haemagglutinin (HA) with Vm‐HA/Protease: Implication for Understanding Bacterial Adherence

Alam

Miyoshi

Ahmed

et al. 2006

Microbiology and Immunology

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Vibrio mimicus (Vm) haemagglutinins (HAs), such as an extracellular HA/protease (Vm-HA/protease) and a major outer membrane protein-HA (Vm-OMPHA), have been recognized as the putative adherence factors for the bacterium. However, the mechanism by which HAs coordinate the adherence function of the bacterium remains as yet unknown. We report herein the positive interaction between Vm-HA/protease and Vm-OMPHA resulting in significant enhancement of the haemagglutinating ability. In this interaction, no cleaved polypeptide was detected; however, limited proteolysis of Vm-OMPHA was confirmed by SDS-PAGE. The proteolytic activation of the native cell-associated Vm-OMPHA by limited proteolysis was also demonstrated in several V. mimicus strains. Proteolytic activation of OMPHA was also achieved with various proteases from bacterial and eukaryotic sources. These findings may indicate a novel coordination of V. mimicus HAs in the adherence of the bacterium.

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Colonization of the rabbit small intestine by clinical and environmental isolates of non-O1 Vibrio cholerae and Vibrio mimicus

Cited by 30 publications

References 21 publications

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Analysis of some factors involved in the virulence mechanism of Vibrio cholerae non-01

Proteolytic Activation of Vibrio mimicus (Vm) Major Outer Membrane Protein Haemagglutinin (HA) with Vm‐HA/Protease: Implication for Understanding Bacterial Adherence

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