Colonization of hospital water systems by Legionella pneumophila, Pseudomonas aeroginosa, and Acinetobacter in ICU wards of Tehran hospitals

Abstract

Hot water from shower heads could be a potential source of infection for Legionella pneumophila. Water was also proved to contain Pseudomonas aeruginonsa, the main GNB-NE causing nosocomila pneumonia at Tehran hospitals. Care should be taken concerning cleanliness and decontamination of water supplies at ICUs for pathogenic organisms.

Nosocomial bacterial infections and their antimicrobial susceptibility patterns among patients in Ugandan intensive care units: a cross sectional study

“…This is in agreement with the EPIC II study in African ICUs especially the proportionately greater number of Acinetobacter species isolates [ 20 ]. Acinetobacter is known to be present in water supplies of hospitals [ 31 ], and contaminates resuscitation equipment and reusable ventilator circuits [ 32 ]. This suggests it can be prevented by simple infection control measures such sterilizing resuscitation equipment, reusable ventilator circuits and avoiding using tap water to flush nasogastric tube [ 20 , 32 ].…”

The €œNEW SETTLERS”: RESULTS OF a BACTERIOLOGICAL SURVEY DURING THE FIRST 6-Months OPERATION PERIOD OF AN INTERNAL MEDICINE WARD IN a TERTIARY HOSPITAL

“…A possible explanation is the examined specimen surfaces selected. The literature describes evidence for presence of pathogenic flora on bed linen (Sui et al, 2012) and water sources (Yaslianifard et al, 2012). It may also be beneficial to sample staff members and their personal equipment (Marinella et al, 1997;Stewart et al, 2011), none of these was examined in the current study.…”

Prevalence ofmipvirulence gene and PCR-base sequence typing ofLegionella pneumophilafrom cooling water systems of two cities in Iran

“…Legionella are presently identified by comparing their 16S rRNA or mip gene sequences, with known sequences deposited in GenBank. 17 To validate the nested -PCR, we performed real-time PCR analysis targeted the mip gene, we found 4 more cases of L. pneumophila corresponds to approximately one-two genome more equivalent per reaction as compared to nested-PCR. Furthermore, real-time PCR offers a rapid amplification of several samples simultaneously and diminishes the likelihood of laboratory contamination.…”

“…[14][15][16] However, current Legionella risk assessments may be compromised by uncertainties in Legionella detection methods. 16,17 There is paucity of information regarding contamination rate of L. pneumophila in water system of different sources in Iran. 17,18 Here, we investigated presence of L. pneumophila mip gene in a total of 128 water samples collected from different hospitals towers, nursing homes and building/hotels water coolants of 2 Iranian cities (Kerman and Bam) during summer season of 2015 (May to August) by both nested and real-time PCR.…”

“…16,17 There is paucity of information regarding contamination rate of L. pneumophila in water system of different sources in Iran. 17,18 Here, we investigated presence of L. pneumophila mip gene in a total of 128 water samples collected from different hospitals towers, nursing homes and building/hotels water coolants of 2 Iranian cities (Kerman and Bam) during summer season of 2015 (May to August) by both nested and real-time PCR. We also studied genetic relationship among the L. pneumophila genomic DNAs from these water samples by PCR-base sequencing method.…”