Colonization of barley roots by Fusarium culmorum and influence of Pseudomonas fluorescens on the process

Струнникова, О. К.; Vishnevskaya, N. A.; Тихонович, И. А.

doi:10.1134/s0026261710060251

Cited by 5 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Joint inoculation of the vermiculite with the pathogen and its antagonist resulted in a greater amount of F. culmorum but a lesser amount of P. fluorescens in barley roots as compared to the mono-inoculation (Table 1). We have already demonstrated that F. culmorum 30 and P. fluorescens 2137 jointly inoculated into the vermiculite successfully colonized the roots and occupied almost the same root zones [19,39]. When both microorganisms were present on the root surface, the amount of each could later fluctuate and, in particular, decrease significantly.…”

Section: Discussionmentioning

confidence: 99%

“…When both microorganisms were present on the root surface, the amount of each could later fluctuate and, in particular, decrease significantly. However, regardless of the fluctuations of the abundance of P. fluorescens on the root surface, its presence always resulted in a decrease in the number of diseased barley plants [18][19][20]. Apparently, the final outcome (the disease incidence in barley) was determined not by the amount of the phytopathogenic fungus and/or antagonistic bacterium in the roots but by their functional activity.…”

Section: Discussionmentioning

confidence: 99%

“…In our earlier studies P. fluorescens strain 2137 actively suppressed the development of F. culmorum 30 both in the soil without plants and in the barley rhizosphere [17,18]. At the same time, we noted that after joint inoculation of the substrate with F. culmorum and P. fluorescens the amount of the fungus on the root surface in the beginning of barley colonization was greater than in case of inoculation with the fungus only [18][19][20]. Later the amount of F. culmorum on barley roots varied considerably both after inoculation with the fungus only and after inoculation with the fungus and the bacterium together.…”

Section: Introductionmentioning

confidence: 92%

“…Later the amount of F. culmorum on barley roots varied considerably both after inoculation with the fungus only and after inoculation with the fungus and the bacterium together. However, an increased amount of F. culmorum on the root surface in the first days of colonization in case of joint inoculation with P. fluorescens was always followed by a considerable decrease in the incidence of Fusarium root rot [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Role of Root Exudates of Barley Colonized by Pseudomonas fluorescens in Enhancing Root Colonization by Fusarium culmorum

Vishnevskaya

Shakhnazarova

Шапошников

et al. 2020

Plants

View full text Add to dashboard Cite

The aim of this study was to find out why after joint inoculation of the substrate with the phytopathogenic fungus Fusarium culmorum and the antagonistic bacterium Pseudomonas fluorescens the amount of the fungus on the root surface in the beginning of the colonization was greater on the roots colonized by the bacterium than on control roots. This phenomenon is especially interesting because joint inoculation with P. fluorescens was always followed by a considerable decrease in the incidence of Fusarium root rot. In two experiments barley was grown in sterile vermiculite inoculated only with F. culmorum, only with P. fluorescens and jointly with the fungus and the bacterium. In the control, vermiculite was not inoculated with any microorganisms. After the removal from the vermiculite, barley plants were transferred into deionized water for the collection of root exudates. The duration of barley growth in the vermiculite and in the water was different in the two experiments. The exudates were tested for their ability to elicit chemotropism in F. culmorum and influence its growth. We did not observe any chemotropism of F. culmorum towards barley root exudates. However, the exudates of the barley colonized by the bacterium stimulated the growth of fungal germ tubes. Using an ultra-performance liquid chromatography system, we found that experimental conditions influenced the quantitative composition of the exudates. The amount of amino acids in the solution of exudates decreased considerably after a prolonged growth of control barley in water, while the presence of P. fluorescens resulted in a considerably increase of the amount of amino acids in the exudates. The exudates of barley colonized by P. fluorescens contained much more glucose, lactic acid and several amino acids than the exudates of control barley. These components are known to be necessary for the growth of F. culmorum. Their presence in the exudates of barley colonized by P. fluorescens seems to be the reason of a more active colonization by the fungus of barley roots colonized by the bacterium.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%