Colonization dynamics of subgingival microbiota in recently installed dental implants compared to healthy teeth in the same individual: a 6-month prospective observational study

Abstract

Objectives To evaluate the colonization dynamics of subgingival microbiota established over six months around newly installed dental implants in periodontally healthy individuals, compared with their corresponding teeth. Methodology Seventeen healthy individuals assigned to receive single dental implants participated in the study. Subgingival biofilm was sampled from all implant sites and contralateral/ antagonist teeth on days 7, 30, 90, and 180 after implant installation. Microbiological analysis was performed using the Checkerboard DNA-DNA hybridization technique for detection of classical oral taxa and non-oral microorganisms. Significant differences were estimated by Mann-Whitney and Friedman tests, while associations between implants/teeth and target species levels were assessed by linear regression analysis (LRA). Significance level was set at 5%. Results Levels of some species were significantly higher in teeth compared to implants, respectively, at day 7 ( V.parvula , 6 vs 3 105 ; Milleri streptococci , 2 vs 6 ; Capnocytophaga spp., 2 vs 9 ; E.corrodens , 2 vs 5 ; N. mucosa , 2 vs 5 ; S.noxia , 2 vs 3 ; T.socranskii , 2 vs 5 ; H.alvei , 4 vs 2 ; and Neisseria spp., 6 vs 4 ), day 30 ( V.parvula , 5 vs 10 5 ; Capnocytophaga spp., 1.3 vs 6.8 ; F.periodonticum , 2 vs 10 6 ; S.noxia , 6 vs 2 ; H.alvei , 8 vs 9 ; and Neisseria spp., 2 vs 10 6 ), day 120 ( V.parvula , 8 vs 3 ; S.noxia ...