Colonization dynamic and distribution of the endophytic fungus Microdochium bolleyi in plants measured by qPCR
Pavel Matušinsky,
Vendula Florová,
Božena Sedláková
et al.
Abstract:Microdochium bolleyi is a fungal endophyte of cereals and grasses proposed as an ideal model organism for studying plant-endophyte interactions. A qPCR-based diagnostic assay was developed to detect M. bolleyi in wheat and Brachypodium distachyon tissues using the species-specific primers MbqITS derived from the ITS of the ribosomal gene. Specificity was tested against 20 fungal organisms associated with barley and wheat. Colonization dynamics, endophyte distribution in the plant, and potential of the seed tra… Show more
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