Colonization and Various Parameters Affecting Egg Hatch and Development of the Old Balara Strain (Philippines), Aedes aegypti

Abstract

Aedes aegypti strain obtained from field collections in Old Balara, Quezon City, Philippines was colonized at the Department of Science and Technology–Philippine Nuclear Research Institute (DOST-PNRI) laboratory. The number of eggs and their weight followed a significant linear relationship for the Ae. aegypti colony. Storing the hatching solution composed of nutrient broth (NB) and brewer’s yeast (BY) longer for at least 16 h hatched the mosquito fastest within 4h. Among the different ages tested, 2-wk-old eggs had the highest hatch rate. After collecting eggs from rearing cages, eggs for 6 wk still produced a high hatch rate and pupal recovery. Mosquito eggs can be effectively stored in cup and ziplock with a hatch rate of at least 77% on the third week, and at least 71% on the fourth week. Eggs stored in plastic cups can be best stored at 17–20 °C in the incubator 4 wk without a decrease in hatch rate. Eggs at these temperatures still produced about 50% egg hatch even until 3 mo.

Ovitrap Monitoring of Aedes aegypti and Aedes albopictus in Two Selected Sites in Quezon City, Philippines

“…This population density would be classified as a level four dengue risk category (40% or above OI, the highest category) based on the classification of Cheung and Fok ( 2009 Hatch rate from field-collected eggs was similar to those reported by Vitek and Livdahl (2006) between the laboratory and field-collected eggs. Pupal recovery (from eggs) was slightly lower than in the laboratory, where the authors typically observe a pupation rate of 87.36 ± 1.24 in colonies established locally (Obra and Javier-Hila 2021). This may be due to insufficient food availability and exposure to varying weather and climatic conditions in the field compared to laboratory conditions.…”

Evaluation of Larval Diets for Mass Rearing of Aedes aegypti L. (Diptera: Culicidae)

“…The stock colony of Ae. aegypti at the Philippine Nuclear Research Institute (PNRI) used in the study was the Old Balara strain, established from collections made in Old Balara, Quezon City (Obra and Javier-Hila, 2021). Ae.…”

“…CF and IAEA diets were given as liquid solutions, while CFM was weighed and given as powder. Eggs were hatched using nutrient broth (M002-500G, HiMedia Laboratories, India) (0.36 g) with BY (0.07 g) dissolved in 1-L dechlorinated water that was stored for 16 h before use (Obra and Javier-Hila, 2021). After 4 h, 100 hatched larvae were counted and placed in a rectangular plastic container measuring 10 x 15 x 3.5 cm with 100-mL dechlorinated water.…”