Colonization and Investigation of Vibrio Cholera Recombination Protein in E-Coli

Abstract

Background and aim: Vibrio cholerae is a gram-negative bacterial pathogen that causes diarrheal disease. One of the most pathogenic factors of V. cholerae is toxin-coregulated pili. This pilus is required as the first factor in the colonization and bacterial persistence in the small intestine. Materials and Methods: In this study, V. cholerae toxin-coregulated pili A (TCPA) gene was amplified using PCR method. The above genes were purified and then expressed by being cloned into the pGEX4T-1 plasmid. Then the recombinant plasmid structure was introduced into the E. coli bacterium. Protein production was carried out by IPTG induction and optimization of culture conditions. The recombinant proteins were purified using Glutathione S-Transferase (GST) Assay Kit and western blot test was then carried out for confirmation of recombinant protein. Protein levels were measured using Bradford protein assay. Results: The results of the present study proved the successful expression of recombinant proteins in E. coli cells. The recombinant protein was purified by affinity chromatography. The reaction pattern between these proteins and their anti-antibodies showed that these proteins have antigenic properties. Conclusion: Since it was proved that these proteins have antigenic properties in this study, they may be used as an appropriate antigen for vaccination of V. cholera.

Promising effects of gingerol against toxins: A review article

“…Environmental changes such as pH or temperature or salt stimulate the onset of gene expression of the bacteria. 92 It has been reported that Vibrio cholera toxin (VCHT) increases the interleukin-6 (IL-6), interleukin-1 (IL-1), and IL-8 secretions. 93,94 This toxin also enhances the release of tumor necrosis factor (TNF-α), 95 extracellular signal-regulated kinases (ERK1/2), and p38 mitogen-activated protein kinases (MAPK).…”

The Investigation of the Corrosive Effects of Sulphates and Salts on the Concrete and the Study of Environmental Changes on it

“…The use of bagasse ashes in the mixing scheme of concrete increases the water absorption and since bagasse ash from Al2O3 and SiO2 It is rich, the hardening process and clamping of concrete samples are accelerated. Also, the presence of calcium oxide the combination of bagasse ash accelerates the process of hardening and stacking of concrete [43][44][45][46][47][48][49][50]. The influence and displacement of chloride ions depends on the structure and pore of concrete.…”

Application of Zeolite Nanofiltration for Removal of Heavy Metals from Urban Wastewater

“…The crystal structure of zeolite nanoparticles was confirmed with the powder's X-ray diffraction (XRD) patterns which were recorded on a PAN analytical instrument Empyrean model with a scanning rate of 0.005˚ 2 min -1 in between 6˚ to 45˚ intervals. The morphologies of the zeolite nanoparticles and porous membranes were characterized by scanning electron microscopy (Quanta 250 FEG) (40)(41)(42)(43)(44). To analyze cross section of the membranes, samples were fractured cryogenically in liquid nitrogen and then subsequently coated with gold using a Magnetron Sputter Coating Instrument for 90 s. EDX was used to evaluate the elemental compositions of the nanoparticles and to determine Si/Al ratio.…”