Colonization and Healthcare-Associated Infection of Carbapenem-Resistant Enterobacteriaceae, Data from Polish Hospital with High Incidence of Carbapenem-Resistant Enterobacteriaceae, Does Active Target Screening Matter?

Pawłowska, Iwona; Ziółkowski, Grzegorz; Jachowicz-Matczak, Estera; Stasiowski, Michał; Gajda, Mateusz; Wójkowska‐Mach, Jadwiga

doi:10.3390/microorganisms11020437

Cited by 2 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main variant of the blaKPC gene detected is blaKPC-2 , with prevalence values varying from 1.2% to 51.6% among enterobacteria in studies carried out in the United States of America and China respectively [34, 35]. This gene is found more frequently in isolates of K. pneumoniae , studies report high prevalence rates in this species ranging from 17.2% to 64.6% [34, 36, 37, 38].…”

Section: Discussionmentioning

confidence: 99%

Molecular insights of β-lactams resistance inKlebsiella pneumoniaeisolates with focus on multidrug resistance and virulence from colonization samples

Nogueira,

Clementino,

Maia

et al. 2023

Preprint

View full text Add to dashboard Cite

Klebsiella pneumoniae is associated with high resistance to antimicrobials and is common in isolates from colonization and nosocomial infections. The study aims to detect resistance genes belonging to the bla family and investigate metabolic pathways in K. pneumoniae isolates. Genes from the subfamilies included: blaSHV, blaTEM, blaNDM, blaKPC, blaGES, blaCTX-M and relevant variants of the blaOXA subfamily. Mass spectrometry data were acquired on the Orbitrap IDX spectrometer (Thermo) connected to the Vanquish UPLC system. Isolates from 122 K. pneumoniae samples were collected from 04/23/2019 to 05/29/2021. A high prevalence of resistance to penicillins, cephalosporins and carbapenems was found among the isolates. The identified genotypic profile showed a high prevalence of genes belonging to Ambler's classes of beta-lactamases A, B and D. In the metabolomic study, the N-fructosyl isoleucine metabolite was identified increased in multidrug-resistant (MDR) strains of K pneumoniae compared to strains susceptible to antimicrobials. In conclusion, the assays developed were efficient in detecting the main genes of the bla family of resistance in K. pneumoniae. The use of the pentose phosphate metabolic pathway suggests the regulation of bacterial growth, virulence, and colonization in MDR K. pneumoniae strains.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular insights of β-lactams resistance inKlebsiella pneumoniaeisolates with focus on multidrug resistance and virulence from colonization samples

Nogueira,

Clementino,

Maia

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Biological specimens considered clinically significant were blood and bronchial lavage cultures. Conversely, colonization status was defined as the isolation of K. pneumoniae from the rectal swab of a patient without clinical signs or symptoms of infection [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

A Whole-Genome Sequencing-Based Approach for the Characterization of Klebsiella pneumoniae Co-Producing KPC and OXA-48-like Carbapenemases Circulating in Sardinia, Italy

Del Rio,

Fox,

Muresu

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Background: Whole-genome sequencing (WGS) provides important information for the characterization, surveillance, and monitoring of antimicrobial resistance (AMR) determinants, particularly in cases of multi- and extensively drug-resistant microorganisms. We reported the results of a WGS analysis carried out on carbapenemases-producing Klebsiella pneumoniae, which causes hospital-acquired infections (HAIs) and is characterized by a marked resistance profile. Methods: Clinical, phenotypic, and genotypic data were collected for the AMR surveillance screening program of the University Hospital of Sassari (Italy) during 2020–2021. Genomic DNA was sequenced using the Illumina Nova Seq 6000 platform. Final assemblies were manually curated and carefully verified for the detection of antimicrobial resistance genes, porin mutations, and virulence factors. A phylogenetic analysis was performed using the maximum likelihood method. Results: All 17 strains analyzed belonged to ST512, and most of them carried the blaKPC-31 variant blaOXA-48-like, an OmpK35 truncation, and an OmpK36 mutation. Phenotypic analysis showed a marked resistance profile to all antibiotic classes, including β-lactams, carbapenems, aminoglycosides, fluoroquinolone, sulphonamides, and novel β-lactam/β-lactamase inhibitors (BL/BLI). Conclusion: WGS characterization revealed the presence of several antibiotic resistance determinants and porin mutations in highly resistant K. pneumoniae strains responsible for HAIs. The detection of blaKPC-31 in our hospital wards highlights the importance of genomic surveillance in hospital settings to monitor the emergence of new clones and the need to improve control and preventive strategies to efficiently contrast AMR.

show abstract

Colonization and Healthcare-Associated Infection of Carbapenem-Resistant Enterobacteriaceae, Data from Polish Hospital with High Incidence of Carbapenem-Resistant Enterobacteriaceae, Does Active Target Screening Matter?

Cited by 2 publications

References 26 publications

Molecular insights of β-lactams resistance inKlebsiella pneumoniaeisolates with focus on multidrug resistance and virulence from colonization samples

Molecular insights of β-lactams resistance inKlebsiella pneumoniaeisolates with focus on multidrug resistance and virulence from colonization samples

A Whole-Genome Sequencing-Based Approach for the Characterization of Klebsiella pneumoniae Co-Producing KPC and OXA-48-like Carbapenemases Circulating in Sardinia, Italy

Contact Info

Product

Resources

About