Colonization and distribution of segmented filamentous bacteria (SFB) in chicken gastrointestinal tract and their relationship with host immunity

Liao, Ningbo; Yin, Yeshi; Sun, Guochang; Xiang, Charlie; Liu, Donghong; Yu, Hongwei D.; Wang, Xin

doi:10.1111/j.1574-6941.2012.01362.x

Cited by 33 publications

(36 citation statements)

References 52 publications

(73 reference statements)

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“…Liao et al. () showed that total bacteria and segmented filamentous bacteria (SBF) in chicken gastrointestinal tract measured by FISH or qPCR were similar, with a slight increase in SBF population quantified by qPCR method. Some authors have shown that next‐generation sequencing (NGS) or qPCR are more precise to detect intestinal bacterial than FISH (Cleusix et al., ).…”

Section: Discussionmentioning

confidence: 99%

Caecal microbiota of chickens fed diets containing propolis

Eyng

Murakami

Pedroso³

et al. 2016

Animal Physiology Nutrition

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The present study aimed to evaluate the effect of different levels of ethanolic extract of propolis (EEP) and raw propolis (RP) on broiler performance and on selected bacterial groups in caecal microbiota using fluorescent in situ hybridization (FISH) measured by fluorescent activated cell sorting. Two experiments were conducted with 120 male chicks from 1 to 21 days of age for each, raised in cages and distributed in a completely randomized experimental design; there were five replicates with four birds per experimental unit and six treatments for each experiment (trial 1 - EEP - 0, 1000, 2000, 3000, 4000 and 5000 ppm and trial 2 - RP - 0, 100, 200, 300, 400 and 500 ppm). Fluorescent probes were used against the bacterial groups in caecal samples collected at 21 days of age. The data were subjected to one-way anova followed by Tukey's and regression analyses were used to assess the relationship between dietary levels of EEP or RP on performance and intestinal microbiota (p < 0.05). In the trial 1, results showed that the EEP did not cause any significant (p > 0.05) modification in the performance and caecal microbiota. In the trial 2, RP inclusion did not affect the performance but changed the bacterial composition (p < 0.05). Clostridiaceae, Gammaproteobacteria excluding Enterobacteriaceae and Lactobacillus spp. showed a quadratic response (p < 0.05), with the lowest value predicted to occur at 240 ppm, 221 ppm and 213 ppm of RP respectively. The proportion of Bacteroidaceae and Gammaproteobacteria did not differ (p > 0.05) among the experimental groups. The inclusion of ethanolic extract of propolis did not affect the performance and intestinal microbiota, whereas the supplementation of raw propolis modulates the caecal microbiota composition without any effects on chicken performance.

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Section: Discussionmentioning

confidence: 99%

Caecal microbiota of chickens fed diets containing propolis

Eyng

Murakami

Pedroso³

et al. 2016

Animal Physiology Nutrition

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“…A higher population of Lactobacillus is particularly associated with SFB-positive Taconic B6 mice. Liao et al (2012) reported that feeding Lactobacillus in drinking water can enhance the earlier colonization of SFB in neonatal chickens. In the current study, qPCR results showed the populations of the total bacteria and other indigenous bacteria to be higher in SFB-positive infants than in SFB-negative infants.…”

Section: Discussionmentioning

confidence: 99%

“…Distribution and diversity of human SFB Y Yin et al the B cells of the host, affects the SFB colonization profoundly (Ohashi et al, 2006;Liao et al, 2012). For mice, the peak number of SFB in the ileum coincides with the window between weaning and the initiation of SIgA self-production.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of the distribution of segmented filamentous bacteria in humans, mice and chickens

et al. 2012

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Segmented filamentous bacteria (SFB) are indigenous gut commensal bacteria. They are commonly detected in the gastrointestinal tracts of both vertebrates and invertebrates. Despite the significant role they have in the modulation of the development of host immune systems, little information exists regarding the presence of SFB in humans. The aim of this study was to investigate the distribution and diversity of SFB in humans and to determine their phylogenetic relationships with their hosts. Gut contents from 251 humans, 92 mice and 72 chickens were collected for bacterial genomic DNA extraction and subjected to SFB 16S rRNA-specific PCR detection. The results showed SFB colonization to be age-dependent in humans, with the majority of individuals colonized within the first 2 years of life, but this colonization disappeared by the age of 3 years. Results of 16S rRNA sequencing showed that multiple operational taxonomic units of SFB could exist in the same individuals. Cross-species comparison among human, mouse and chicken samples demonstrated that each host possessed an exclusive predominant SFB sequence. In summary, our results showed that SFB display host specificity, and SFB colonization, which occurs early in human life, declines in an age-dependent manner.

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“…SFB are found principally in the ileal mucus with some presence in ileal content (63) although there is one report of SFB in the caecal tonsils (64). SFB appear during the first week of life, reaching peak abundance between 9 and 14 d.p.h before declining (63,64). The pattern of colonization varies greatly among individual birds, especially at an early age.…”

Section: Candidatus Arthromitus: Colonization and Immune Developmentmentioning

confidence: 99%

Development of the Ileal Microbiota in Three Broiler Breeds

et al. 2020

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The development and succession of the microbiota in ileal mucus and lumen samples from three breeds of broiler chicken (Cobb 500, n = 36; Hubbard JA87, n = 38; and Ross 308, n = 36) was observed between 3 and 42 days post hatch (d.p.h). Chicks were housed in the same room of a climate-controlled, biosecure chicken housing unit. Between 0 and 14 d.p.h, chicks were kept in three circular brooder pens ensuring a mixture of breeds in each brooder. From 22 d.p.h, chicks were removed from the brooders and kept in the same room. DNA was extracted from a pooled sample of ileal mucus and luminal contents taken from five birds of each breed at 3, 7, 14, 21, 28, and 42 d.p.h. High-throughput Illumina sequencing was performed for the V4 hypervariable region of the 16S rRNA gene. The initial microbiota in the ileum varied between breeds. The common features were a low diversity and general dominance by one or two taxa such as Enterococcus or Escherichia with relatively low numbers of Lactobacillus. Escherichia became the most abundant genus in samples where Enterococcus was previously the dominant taxa. The next phase of development was marked by an increase in the abundance of Candidatus Arthromitus in the mucus and Lactobacillus in the lumen. The high abundance of Candidatus Arthromitus persisted between 7 and 14 d.p.h after which Lactobacillus became the most abundant genus in both the mucus and lumen. Dominance of the ileal microbiota by Lactobacillus was a transient feature. By 42 d.p.h, the relative abundance of Lactobacillus had fallen while a range of other taxa including Escherichia, Turicibacter, and members of Clostridiales increased. This general pattern was followed by all breeds, however, the rate at which succession occurred differed as Ross matured quicker than Cobb with Hubbard as an intermediate.

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Colonization and distribution of segmented filamentous bacteria (SFB) in chicken gastrointestinal tract and their relationship with host immunity

Cited by 33 publications

References 52 publications

Caecal microbiota of chickens fed diets containing propolis

Caecal microbiota of chickens fed diets containing propolis

Comparative analysis of the distribution of segmented filamentous bacteria in humans, mice and chickens

Development of the Ileal Microbiota in Three Broiler Breeds

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