Colonization and Distribution ofXanthomonas phaseoliandXanthomonas phaseolivar.fuscansin Field-Grown Navy Beans

Weller, David M.; Saettler, A. W.

doi:10.1094/phyto-70-500

Cited by 56 publications

(41 citation statements)

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“…Photoassimilates like sucrose, fructose, and glucose, which are found in abundances of 0.2-2.0 g per leaf on uninhabited bean leaf surfaces, were readily consumed and converted into biomass by the bacterium Pseudomonas fluorescens A506 (24). Bacterial and fungal colonization of the phyllosphere does not occur evenly across the leaf (25,26), suggesting that resources are not evenly available or exploitable. Microscopic analyses have revealed that bacteria are more likely to be found clustered in crevices between epidermal cells, near the base of trichomes, in the proximity of stomates, and along veins (27)(28)(29).…”

mentioning

confidence: 99%

Appetite of an epiphyte: Quantitative monitoring of bacterial sugar consumption in the phyllosphere

Leveau

Lindow

2001

Proc. Natl. Acad. Sci. U.S.A.

308

289

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We report here the construction, characterization, and application of a bacterial bioreporter for fructose and sucrose that was designed to monitor the availability of these sugars to microbial colonizers of the phyllosphere. Plasmid pPfruB-gfp[AAV] carries the Escherichia coli fruB promoter upstream from the gfp[AAV] allele that codes for an unstable variant of green fluorescent protein (GFP). In Erwinia herbicola, this plasmid brings about the accumulation of GFP fluorescence in response to both fructose and sucrose. Cells of E. herbicola (pP fruB-gfp[AAV]) were sprayed onto bean plants, recovered from leaves at various time intervals after inoculation, and analyzed individually for GFP content by quantitative analysis of digital microscope images. We observed a positive correlation between single-cell GFP accumulation and ribosomal content as determined by fluorescence in situ hybridization, indicating that foliar growth of E. herbicola occurred at the expense of fructose and͞or sucrose. One hour after inoculation, nearly all bioreporter cells appeared to be actively engaged in fructose consumption. This fraction dropped to approximately 11% after 7 h and to Ϸ1% a day after inoculation. This pattern suggests a highly heterogeneous availability of fructose to individual E. herbicola cells as they colonize the phyllosphere. We estimated that individual cells were exposed to local initial fructose abundances ranging from less than 0.15 pg fructose to more than 4.6 pg.

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mentioning

confidence: 99%

Appetite of an epiphyte: Quantitative monitoring of bacterial sugar consumption in the phyllosphere

Leveau

Lindow

2001

Proc. Natl. Acad. Sci. U.S.A.

308

289

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“…Xap and Xff survive epiphytically until favorable conditions for infection are present (Weller and Saettler, 1980b). These bacteria are well adapted to survive harsh phyllosphere conditions following epiphytic aggregation in biofilms (Jacques et al, 2005).…”

Section: Infectionmentioning

confidence: 99%

“…Bacteria penetrate into the seed through the funiculus and the micropyle (Zaumeyer, 1930). External contamination of seeds occurs from contaminated pods and during threshing and cleaning (Weller and Saettler, 1980b).…”

Section: Infectionmentioning

confidence: 99%

Scientific Opinion on the pest categorisation ofXanthomonas axonopodispv.phaseoliandXanthomonas fuscanssubsp.fuscans

2014

EFSA Journal

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The European Commission requested the EFSA Panel on Plant Health to perform a pest categorisation for Xanthomonas campestris pv. phaseoli. In reaching its conclusions, the Panel considered four parameters to be of critical importance in the risk assessment area: (i) the presence and distribution of X. campestris pv. phaseoli in the risk assessment area and its potential for spread; (ii) the existence and the distribution of the main host plants throughout the European Union (EU); (iii) the potential for damage to crops grown in Europe; and (iv) the implementation of pest control strategies and cultural measures. The organisms formerly identified as X. campestris pv. phaseoli have proven to be genetically diverse and are currently known as Xanthomonas fuscans subsp. fuscans and Xanthomonas axonopodis pv. phaseoli. Based on its analysis, the Panel concluded that the identity of these pathogens is clearly defined and their distribution involves limited areas or regions in the EU, although with a medium uncertainty. The main dispersal pathway for these pathogens is contaminated seeds. In addition, the Panel noted that host plants are widely cropped throughout the EU, common bean Phaseolus vulgaris being of great commercial importance, not only for consumption-fresh, canned, deep frozen and dried-but also for the seed industry. Currently, the impact of common bacterial blight is limited in the EU as a consequence of seed testing. Finally, the Panel concluded that moderate to high consequences, in terms of yield and quality losses, are expected for beans, especially if weather conditions are conducive to disease and seed inoculum no longer contained. © European Food Safety Authority, 2014 KEY WORDSXanthomonas campestris pv. phaseoli, Xanthomonas axonopodis pv. phaseoli, Xanthomonas fuscans subsp. fuscans, common bacterial blight of beans, bacterial leaf pustule and fuscous blight of bean, quarantine pest, regulated non-quarantine pest

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Section: Introductionmentioning

confidence: 99%

“…Seed transmission is the primary means by which the pathogen is disseminated [13][14][15]. Internally and externally infested seeds are important sources of primary inocula for Xap [14,16]. Sutton and Wallen [17] reported that approximately one diseased seed in 10000 seeds are capable of causing an outbreak of blight.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Importance of Xanthomonas axonopodis pv. Phaseoli in Common Bean (Phaseolus vulgaris L) Seed Produced under Different Seed Production System in Central Rift Valley of Ethiopia

Leta¹,

Lamessa²,

Ayana³

2017

J Plant Pathol Microbiol

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Colonization and Distribution ofXanthomonas phaseoliandXanthomonas phaseolivar.fuscansin Field-Grown Navy Beans

Cited by 56 publications

References 0 publications

Appetite of an epiphyte: Quantitative monitoring of bacterial sugar consumption in the phyllosphere

Appetite of an epiphyte: Quantitative monitoring of bacterial sugar consumption in the phyllosphere

Scientific Opinion on the pest categorisation ofXanthomonas axonopodispv.phaseoliandXanthomonas fuscanssubsp.fuscans

Occurrence and Importance of Xanthomonas axonopodis pv. Phaseoli in Common Bean (Phaseolus vulgaris L) Seed Produced under Different Seed Production System in Central Rift Valley of Ethiopia

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