Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.)

Ferguson, Yvonne

doi:10.1016/s0168-6496(98)00071-3

Cited by 4 publications

(5 citation statements)

References 20 publications

(28 reference statements)

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“…In fish, Aeromonas salmonicida expressing luxAB was used to monitor colonization and transmission of the pathogen in Atlantic salmon ( Salmo salar ), but bioluminescence was not detected in live fish (Ferguson et al , 1998). In addition, luxAB marked A. salmonicida required exogenous addition of aldehyde substrate for luminescence (Ferguson et al , 1998). We have previously used luminescent E. ictaluri ( luxCDABE carried on a ColE1 plasmid) for in vitro quantification of E. ictaluri serum resistance (Lawrence et al , 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Real-time monitoring of bacterial infection using BLI in a living host has been used mainly in mouse models (Contag et al, 1995;Forde et al, 1998). In fish, Aeromonas salmonicida expressing luxAB was used to monitor colonization and transmission of the pathogen in Atlantic salmon (Salmo salar), but bioluminescence was not detected in live fish (Ferguson et al, 1998). In addition, luxAB marked A. salmonicida required exogenous addition of aldehyde substrate for luminescence (Ferguson et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

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Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring

Karsi

Menanteau–Ledouble

Lawrence

2006

FEMS Microbiology Letters

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Edwardsiella ictaluri is a facultative intracellular bacterium that causes enteric septicemia of catfish (ESC). In this study, we aimed to develop bioluminescent E. ictaluri that can be monitored by noninvasive bioluminescence imaging (BLI). To accomplish this, the luxCDABE operon of Photorhabdus luminescens was cloned downstream of the lacZ promoter in the broad host range plasmid pBBR1MCS4. Edwardsiella ictaluri strain 93-146 transformed with the new plasmid, pAKlux1, was highly bioluminescent. pAKlux1 was stably maintained in E. ictaluri without any apparent effect on growth or native plasmid stability. To assess the usefulness of the bioluminescent strain in disease studies, catfish were infected with 93-146 pAKlux1 by intraperitoneal injection and by bath immersion, and in vivo bacterial dissemination was observed using BLI. This study demonstrated that bioluminescent E. ictaluri can be used for real-time monitoring of ESC in live fish, which should enable observation of pathogen attachment sites and tissue predilections.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring

Karsi

Menanteau–Ledouble

Lawrence

2006

FEMS Microbiology Letters

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“…Several studies have suggested that skin, lateral line, gills and GI tract (or a combination of these organs) are infection routes for pathogenic bacteria. In a study on Atlantic salmon Salmo salar L., Ferguson, Bricknell, Glover, MacGregor & Prosser (1998) suggested that the gills and skin/mucus regions were the main sites for attachment of A. salmonicida. Here, we have clearly demonstrated that A. salmonicida ssp.…”

Section: Discussionmentioning

confidence: 99%

The effects of soybean, linseed and marine oils on aerobic gut microbiota of Arctic charrSalvelinus alpinusL. before and after challenge withAeromonas salmonicidassp.salmonicida

Ringø

Lødemel

Myklebust

et al. 2002

Aquaculture Research

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Populations of heterotrophic bacteria present in the hindgut region of Arctic charr Salvelinus alpinus L. fed dietary soybean, linseed and marine oils before challenge with Aeromonas salmonicida ssp. salmonicida and marine oil after challenge were estimated using the dilution plate technique. There were differences in bacterial composition between the rearing groups before and after challenge, as well as interindividual variations. For example, carnobacteria were only isolated from the hindgut region of fish fed soybean oil and linseed oil before challenge, whereas Carnobacterium spp. and Carnobacterium funditum‐like species were isolated from fish fed the same oils after challenge. Three non‐motile Aeromonas spp. were isolated from infected fish fed marine oil. One of these isolates was identified as identical to A. salmonicida ssp. salmonicida used in&the challenge test by microbial fingerprinting (amplified fragment length polymorphism). Electron microscopic examinations of hindgut regions demonstrated substantial numbers of bacterial cells associated with enterocytes, but bacterial colonization of the enterocyte surface varied between different rearing groups. The potential of bacteria found associated with the hindgut region to inhibit the fish pathogens A. salmonicida, Vibrio salmonicida and Vibrio anguillarum differed between rearing groups.

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“…However, as demonstrated earlier in this study, Digestarom ® has a direct antimicrobial effect, and because it was delivered as a feed supplement it seems likely that this effect would be most efficient within the digestive track. A. salmonicida, on the other hand, can infect the fish through the intestine (Jutfelt et al 2006), gills or epithelium (Svendsen & Bøgwald 1997, Ferguson et al 1998. Therefore, the antimicrobial effect of Digestarom ® is likely to play an important protective role in fish exposed through natural routes of exposure, whereas injecting the bacterial solution intraperitoneally would bypass this protective effect.…”

Section: In Vivo Challenges With Aeromonas Salmonicidamentioning

confidence: 99%

Effect of a phytogenic feed additive on the susceptibility of Onchorhynchus mykiss to Aeromonas salmonicida

Menanteau–Ledouble¹,

Krauss²,

Santos³

et al. 2015

Dis. Aquat. Org.

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In recent years, feed additives have increasingly been adopted by the aquaculture industry. These supplements not only offer an alternative to antibiotics but have also been linked to enhanced growth performance. However, the literature is still limited and provides contradictory information on their effectiveness. This is mainly due to the wide variety of available products and their complex mechanisms of action. Phytogenic feed additives have been shown to have antimicrobial effects and can improve growth performance. In the present study, we investigated the susceptibility of several fish pathogenic bacteria to a phytogenic essential oil product in vitro. In addition, we determined the protective effect of a commercial phytogenic feed additive containing oregano, anis and citrus oils on the resistance of rainbow trout Oncorhynchus mykiss to infection by Aeromonas salmonicida. The bacterium was administered through 3 different routes: intra-peritoneal injection, immersion in a bacterial solution and cohabitation with infected fish. Mortality rates were significantly lower in infected rainbow trout that had received the feed additive: the overall mortality rate across all routes of infection was 18% in fish fed a diet containing the additive compared to 37% in fish that received unsupplemented feed. The route of infection also significantly impacted mortality, with average mortality rates of 60, 17.5 and 5% for intraperitoneal injection, immersion and cohabitation, respectively. In general, fish were better protected against infection by immersion than infection by injection.

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Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.)

Cited by 4 publications

References 20 publications

Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring

Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring

The effects of soybean, linseed and marine oils on aerobic gut microbiota of Arctic charrSalvelinus alpinusL. before and after challenge withAeromonas salmonicidassp.salmonicida

Effect of a phytogenic feed additive on the susceptibility of Onchorhynchus mykiss to Aeromonas salmonicida

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