Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome

Abstract

Recent studies on the pathophysiology of irritable bowel syndrome (IBS) have focused on the role of mast cells (MCs) in intestinal mucosal immunity. A link between allergic airway diseases (AADs) and IBS has been suggested because both diseases have similar pathophysiology. We aimed to investigate whether the induction of AAD in mice could lead to inflammation of the colonic mucosa, similar to IBS. We also evaluated whether this inflammatory response could be suppressed by administering a therapeutic agent. Mice were divided into three groups: control, AAD-induced, and salbutamol-treated. An AAD mouse model was established by intraperitoneal injection and nasal challenge with ovalbumin. Mice with AAD were intranasally administered salbutamol. Analyses of cytokine levels, MC count, and tryptase levels in the intestinal mucosa were performed to compare the changes in inflammatory responses among the three groups. Inflammation was observed in the intestinal mucosa of mice in the AAD group. This inflammation in AAD mice was suppressed after salbutamol treatment. Our study demonstrates that AAD induces an inflammatory response similar to that in IBS, suggesting a possible association between IBS and AADs. In patients with IBS with such allergic components, salbutamol may have the potential to alleviate the inflammatory response.

Sirtuin 7 Inhibitor Attenuates Colonic Mucosal Immune Activation in Mice—Potential Therapeutic Target in Inflammatory Bowel Disease

“…In our preliminary study, we confirmed that SIRT7 was elevated in the lungs of mice that induced allergic inflammation of the lung and hypothesized that a similar response would also occur in the colon mucosa that induced colitis [ 15 ]. We further evaluated whether SIRT7 inhibitors attenuated inflammation in a colitis model.…”

“…In an OVA-induced colitis model, OVA simultaneously induced colitis and allergic inflammation in the lungs. We induced inflammation in the lungs and colons of female BALB/c mice and performed multiple tests to confirm that the OVA-induced lung inflammation/colitis model was successfully established [ 15 ]. We decided to analyze SIRT7 in lung allergic inflammation as a preliminary study before colon evaluation.…”

“…In this experiment, we used the OVA-induced inflammation murine model used in our previous study [ 15 ]. Briefly, it is a model wherein OVA simultaneously induces inflammation in the lungs and intestines through immune migration.…”

“…All experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, the ARRIVE guidelines, and other relevant guidelines and regulations. In this study, we used the OVA-induced colitis murine model used in our previous studies [ 15 ].…”

“…We previously confirmed subsequent inflammatory changes in the large intestine when inflammatory reactions were induced in the lungs of the mice by nasal stimulation of ovalbumin (OVA); we named this the “ovalbumin-induced colitis model” [ 15 ]. In our preliminary study, using an OVA-induced colitis model, we found that SIRT7 levels in the lungs were increased when inflammatory responses were induced.…”