Colonic expression of calcium transporter TRPV6 is regulated by dietary sodium butyrate

Gommers, Lisanne M. M.; Wijst, Jenny van der; Bos, Caro; Janssen, Luuk; Bindels, René J. M.; Baaij, Jeroen H. F. de; Hoenderop, Joost G. J.

doi:10.1007/s00424-021-02648-6

Cited by 3 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the fact that Mg 2+ absorption via TRPM6/7 channels is high in the proximal colon, and this intestinal segment is dominated by butyrate-producing bacteria 10,47,48 , butyrate could act as negative regulator of Mg 2+ absorption in this segment. Although butyrate supplementation did not result in differences in serum Mg 2+ concentration or fecal Mg 2+ extrusion between butyrate and control-treated mice after 1 and 14 days of treatment in a previous study 49 , our correlation analyses demonstrated that the colonic butyrate content is inversely associated with the serum Mg 2+ levels in a physiologically relevant setting.…”

Section: Discussioncontrasting

confidence: 60%

Butyrate reduces cellular magnesium absorption independently of metabolic regulation in Caco-2 human colon cells

Gommers

Leermakers

Wijst

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Digestion of dietary fibers by gut bacteria has been shown to stimulate intestinal mineral absorption [e.g., calcium (Ca2+) and magnesium (Mg2+)]. Although it has been suggested that local pH and short-chain fatty acid (SCFA) concentrations determine divalent cation absorption, the exact molecular mechanisms are still unknown. Therefore, this study aimed to determine the effects of SCFAs on intestinal Mg2+ absorption. We show that the butyrate concentration in the colon negatively correlates with serum Mg2+ levels in wildtype mice. Moreover, Na-butyrate significantly inhibited Mg2+ uptake in Caco-2 cells, while Ca2+ uptake was unaffected. Although Na-butyrate significantly lowered total ATP production rate, and resulted in increased phosphorylation of AMP-activated protein kinase (AMPK), inhibition of Mg2+ uptake by butyrate preceded these consequences. Importantly, electrophysiological examinations demonstrated that intracellular butyrate directly reduced the activity of the heteromeric Mg2+ channel complex, transient receptor potential melastatin (TRPM)6/7. Blocking cellular butyrate uptake prevented its inhibitory effect on Mg2+ uptake, demonstrating that butyrate acts intracellularly. Our work identified butyrate as novel regulator of intestinal Mg2+ uptake that works independently from metabolic regulation. This finding further highlights the role of microbial fermentation in the regulation of mineral absorption.

show abstract

Section: Discussioncontrasting

confidence: 60%

Butyrate reduces cellular magnesium absorption independently of metabolic regulation in Caco-2 human colon cells

Gommers

Leermakers

Wijst

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Supplementary SB can improve the performance of broilers by improving intestinal development ( Liu et al, 2017 ). Dietary butyrate has a regulatory effect on the transport of mineral elements ( Gommers et al, 2022 ), while SB improves egg production and shell strength and reduces feed conversion ( Wang et al, 2021b ; Zhang et al, 2022b ). Feeding SB for 5 wk improved the immunity and growth potential of 40-wk-old laying hens’ offspring ( Gong et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Dietary sodium butyrate improves female broiler breeder performance and offspring immune function by enhancing maternal intestinal barrier and microbiota

Xiao¹,

Zhang²,

Zhang³

et al. 2023

Poultry Science

View full text Add to dashboard Cite

Soluble corn fiber, resistant corn starch, and protected butyrate effects on performance, gastrointestinal volatile fatty acids, and apparent total-tract digestibility of calcium and phosphorus in nursery pigs

Kerr

Pearce

Ramirez

et al. 2023

Journal of Animal Science

View full text Add to dashboard Cite

An experiment was conducted to determine how feeding a calcium (Ca) deficient diet would affect gastrointestinal pH and volatile fatty acids (VFA), Ca digestibility, bone mineral density (BMD), and performance in nursery pigs; and if supplementation of nondigestible oligosaccharides would affect these same parameters. Two hundred and forty weaned pigs (BW = 7.1 kg) were placed into 80 pens with 3 pigs/pen. The 8 dietary treatments consisted of: 1) positive control (PC, 0.83% total Ca), 2) negative control (NC, 0.50% total Ca), 3 and 4) NC + 5% or 7.5% soluble corn fiber (SCF), 5 and 6) NC + 5% or 7.5% resistant corn starch (rCS), 7 and 8) NC + 0.25% or 0.50% fat-protected butyrate (pBRT). Pigs were ad libitum fed the dietary treatments for 21 d to determine average daily gain (ADG), average daily feed intake (ADFI) and gain:feed ratio (GF) with a fecal sample collected from each pen to determine Ca digestibility using acid insoluble ash as the dietary marker, with 1 pig/pen euthanized on d 21 for collection of ileal and colon contents and the left humerus. Pigs fed the NC diet had a lower colonic pH compared to pigs fed the PC (P = 0.06) but no effect on total VFA was observed (P > 0.10). Pigs fed diets containing SCF and rCS had lower colonic pH and total VFA compared to pigs fed the NC diet (P ≤ 0.05). Pigs fed diets containing pBRT had greater colonic total VFA compared to pigs fed the NC diet (P ≤ 0.07), but no difference in colonic pH was observed (P > 0.10). Pigs fed the NC diet had a greater Ca digestibility compared to pigs fed the PC (P ≤ 0.01), with no treatment to the NC having any effect on Ca digestibility compared to pigs fed the NC (P > 0.10). There was no effect of dietary Ca level on BMD and no overall addition of feeding SCF, rCS, or pBRT on BMD compared to pigs fed the NC (P > 0.10). There was no impact on pig ADG, ADFI, or GF by reducing dietary Ca by 40% (i.e., pigs fed the NC) compared to pigs fed the PC (P > 0.10). Relative to pigs fed the NC, there was no overall effect of SCF, rCS, or pBRT on ADG, ADFI, or GF (P > 0.10). In conclusion, feeding young pigs a Ca-deficient diet reduced colonic pH, increased digestibility of Ca, but had no impact on bone mineralization or overall pig performance. Supplementation of nondigestible oligosaccharides pr protected butyrate had either no effect or an inconsistent effect on colonic pH, Ca or PHOS digestibility, bone mineralization, or overall pig performance.

show abstract

Colonic expression of calcium transporter TRPV6 is regulated by dietary sodium butyrate

Cited by 3 publications

References 35 publications

Butyrate reduces cellular magnesium absorption independently of metabolic regulation in Caco-2 human colon cells

Butyrate reduces cellular magnesium absorption independently of metabolic regulation in Caco-2 human colon cells

Dietary sodium butyrate improves female broiler breeder performance and offspring immune function by enhancing maternal intestinal barrier and microbiota

Soluble corn fiber, resistant corn starch, and protected butyrate effects on performance, gastrointestinal volatile fatty acids, and apparent total-tract digestibility of calcium and phosphorus in nursery pigs

Contact Info

Product

Resources

About