Colonic epithelium reactive monoclonal antibodies

Verstijnen, C.; Arends, Jan Willem; Moerkerk, Peter T.M.; Pijls, M M; Kuypers-Engelen, B.T.M.J.; Bosman, Fred T.

doi:10.1007/bf00492497

Cited by 6 publications

(1 citation statement)

References 35 publications

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“…Immunohistochemically, the tumors showed reactivity for various mucin-associated antibodies. Both expression of Parlam 319, identifying a protein moiety of glycoproteins present in normal goblet cells, and of Parlam 13, identifying a carbohydrate epitope in glycoproteins increasingly expressed in neoplastic colonic epithelium [50], were found ( Fig. 6a-d).…”

Section: Caco-2 Ej6 Cellsmentioning

confidence: 98%

Human Caco-2 cells transfected with c-Ha-Ras as a model for endocrine differentiation in the large intestine

et al. 1993

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Section: Caco-2 Ej6 Cellsmentioning

confidence: 98%

Human Caco-2 cells transfected with c-Ha-Ras as a model for endocrine differentiation in the large intestine

et al. 1993

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Clonal origin of trisomy for chromosome 7 in the epithelial compartment of colon neoplasia

Herbergs

Arends

Bongers

et al. 1996

Genes Chromosom. Cancer

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In this study, we demonstrated the clonal origin of trisomy for chromosome 7 in epithelial cells of colon neoplasia. By using the double‐target fluorescence in situ hybridization (FISH) technique in frozen tissue sections that were also immunostained for keratin and vimentin, ratio analysis of FISH signals for chromosomes 7 and 17 could be performed in epithelial (cytokeratin‐positive) or stromal (vimentin‐positive) areas. The data demonstrated that trisomy for chromosome 7 is found exclusively in the epithelial compartments and not in the stroma of colon adenocarcinoma. We then demonstrated the occurrence of trisomy for chromosome 7 in the different types of epithelial neoplastic cells, i.e., columnar and goblet cells, which were isolated from frozen tissue sections by mechanical disaggregation of colon tissue and mild lysis of the cells while protease activity was inhibited. In these cell suspensions, the columnar cells were detected with an antibody to villin, and the goblet cells were stained for mucin, whereas all cells were subsequently subjected to FISH for chromosome 7. For analysis of neuroendocrine cells, which are present in a very low frequency in colon neoplasia, frozen tissue sections that were immunostained for Chromogranin A could be used. Individual neuroendocrine cells could be distinguished in these thin frozen tissue sections. The presence of trisomy for chromosome 7 in all three different epithelial cell types strengthens our suggestion that this chromosomal aberration is found in the epithelial stem cell compartment of colon neoplasia. Genes Chromosom Cancer 16:106–112 (1996). © 1996 Wiley‐Liss, Inc.

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Endocrine cells in colorectal adenocarcinomas: Incidence, hormone profile and prognostic relevance

Bruïne¹,

Wiggers

Beek³

et al. 1993

Intl Journal of Cancer

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The occurrence of endocrine cells in 350 cases of colorectal adenocarcinoma was studied by immunohistochemistry for chromogranin A (CGA). The hormone profile of endocrine tumor cells, the correlation between endocrine differentiation and presence of other colorectal epithelial-cell lineages and the prognostic relevance of endocrine differentiation in colorectal cancer were investigated. CGA-positive tumor cells were found in 30% of cases, 21% showing moderate positivity and 9.0% extensive positivity. Of CGA-positive tumors, 70% additionally produced neurohormones, mainly indigenous to normal colorectal epithelium: 55% showed immunoreactivity for glucagon-like substances, 20% for serotonin and 10% for somatostatin, PYY and HCG. No immunoreactivity was found for various neurohormones not normally produced by colorectal endocrine cells. CGA-positive tumors tended to be more aggressive than CGA-negative tumors. Especially, tumors with extensive CGA positivity showed shorter survival, which was most apparent within Dukes' stage C. In multivariate analysis, extensive CGA positivity was an independent indicator of poor prognosis. CGA immunoreactivity significantly correlated with mucin production, but not with expression of secretory component (SC), a columnar-cell marker. Mucin production significantly correlated with SC expression. Tumors positive for CGA but not for mucin and/or SC showed the worst prognosis. SC expression was a relatively favorable feature, and mucin-producing tumors showed intermediate behavior.

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Colonic epithelium reactive monoclonal antibodies

Cited by 6 publications

References 35 publications

Human Caco-2 cells transfected with c-Ha-Ras as a model for endocrine differentiation in the large intestine

Human Caco-2 cells transfected with c-Ha-Ras as a model for endocrine differentiation in the large intestine

Clonal origin of trisomy for chromosome 7 in the epithelial compartment of colon neoplasia

Endocrine cells in colorectal adenocarcinomas: Incidence, hormone profile and prognostic relevance

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