ColonialDifferentiation in
 Streptomyces
 coelicolor
 Depends on Translation of a SpecificCodon within the
 adpA
 Gene

Nguyen, Kien T.; Tenor, Jennifer L.; Stettler, Hansruedi; Nguyen, Lieu Thi; Nguyen, Liem; Thompson, Charles J.

doi:10.1128/jb.185.24.7291-7296.2003

Cited by 72 publications

(96 citation statements)

References 20 publications

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“…From the 145 genes that possess a TTA codon, 33 at least one, adpA (bldH), is required for sporulation on glucose-containing media. 34,35 This suggests that the ability of iron to promote development must act upstream of the adpA regulatory network. The failure of iron to recover actinorhodin production is readily explained by the presence of a TTA codon within the sequence of the pathwayspecific activator actII-ORF4 gene which is required for activation of actinorhodin biosynthesis, as well as in actII-ORF2 for actinorhodin export.…”

Section: S Coelicolor Bld Mutants Are Affected In Siderophore Biosynmentioning

confidence: 99%

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

et al. 2014

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Streptomyces coelicolor is an important model organism for developmental studies of filamentous GCrich actinobacteria. The genetic characterization of mutants of S. coelicolor blocked at the vegetative mycelium stage, the so-called bald (bld) mutants that are unable to erect spore-forming aerial hyphae, has opened the way to discovering the molecular basis of development in actinomycetes. Desferrioxamine (DFO) production and import of ferrioxamines (FO; iron-complexed DFO) are key to triggering morphogenesis of S. coelicolor and we show here that growth of S. coelicolor on the reference medium for Streptomyces developmental studies is fully dependent on DFO biosynthesis. UPLC-ESI-MS analysis revealed that all bld mutants tested are affected in DFO biosynthesis, with bldA, bldJ, and ptsH mutants severely impaired in DFO production, while bldF, bldK, crr and ptsI mutants overproduce DFO.Morphogenesis of bldK and bldJ mutants was recovered by supplying exogenous iron. Transcript analysis showed that the bldJ mutant is impaired in expression of genes involved in the uptake of FO, whereas transcription of genes involved in both DFO biosynthesis and FO uptake is increased in bldK mutants. Our study allows proposing altered DFO production and/or FO uptake as a novel phenotypic marker of many S. coelicolor bld mutants, and strengthens the role of siderophores and iron acquisition in morphological development of actinomycetes.

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Section: S Coelicolor Bld Mutants Are Affected In Siderophore Biosynmentioning

confidence: 99%

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

et al. 2014

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“…A search of the S. coelicolor genome using nrdS as the query revealed 10 ORFs sharing 48-34% sequence identity. One, SCO2792, is a close homologue of the Streptomyces griseus AdpA transcriptional regulator (84.4% identity between the gene products) (Ohnishi et al, 1999;Horinouchi, 2002) and has recently been shown to be necessary for normal S. coelicolor aerial mycelium formation: it is the bldH gene (Nguyen et al, 2003;Takano et al, 2003). A second, SCO7512, is a close homologue of the Streptomyces aureofaciens GapR regulator (89% identity between the gene products) (Sprusansky et al, 2001).…”

Section: The S Coelicolor Class Ia and Class II Rnr Transcription Unmentioning

confidence: 99%

Alternative oxygen‐dependent and oxygen‐independent ribonucleotide reductases in Streptomyces: cross‐regulation and physiological role in response to oxygen limitation

Borovok

Gorovitz

Yanku

et al. 2004

Molecular Microbiology

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SummaryRibonucleotide reductases (RNRs) catalyse the conversion of ribonucleotides to deoxyribonucleotides and are essential for de novo DNA synthesis and repair. Streptomyces spp. contain genes coding for two RNRs. We show here that the Streptomyces coelicolor M145 nrdAB genes encoding an oxygendependent class I RNR are co-transcribed with nrdS , which encodes an AraC-like regulatory protein. Likewise, the class II oxygen-independent RNR nrdJ gene forms an operon with a likely regulatory gene, nrdR , which encodes a protein possessing an ATP-cone domain like those present in the allosteric activity site of many class Ia RNRs. Deletions in nrdB and nrdJ had no discernible effect on growth individually, but abolition of both RNR systems, using hydroxyurea to inactivate the class Ia RNR (NrdAB) in the nrdJ deletion mutant, was lethal, establishing that S. coelicolor possesses just two functional RNR systems. The class II RNR (NrdJ) may function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen, as the nrdJ mutant was slower in growth recovery than the nrdB mutant or the parent strain. The class Ia and class II RNR genes show complex regulation. The nrdRJ genes were transcribed some five-to sixfold higher than the nrdABS genes in vegetative growth, but when nrdJ was deleted, nrdABS transcription was upregulated by 13-fold. In a reciprocal experiment, deletion of nrdB had little effect on nrdRJ transcription. Deletion of nrdR caused a dramatic increase in transcription of nrdJ and to a less extent nrdABS , whereas disruption of cobN , a gene required for synthesis of coenzyme B12 a cofactor for the class II RNR, caused similar upregulation of transcription of nrdRJ and nrdABS . In contrast, deletion of nrdS had no detectable effect on transcription of either set of RNR genes. These results establish the existence of control mechanisms that sense and regulate overall RNR gene expression.

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“…Previously, a bioassay method based on the pigment production by S. coelicolor was developed as a convenient assay for some GBL molecules (15). When we applied S. venezuelae culture extracts harvested at different times to S. coelicolor indicator plates, several fractions (12,24,30,36, and 42 h) were observed to induce pigment production (Fig. S1).…”

Section: Resultsmentioning

confidence: 99%

“…1A) and the fact that ScbR2 shows ∼31% identity to ArpA of Streptomyces griseus-which was known to bind the promoter region of adpA to repress its transcription (22)-we also tested the binding of ScbR2 to the promoter of adpA (adpAp), the product of which is the master regulator of aerial hyphal production in S. coelicolor (23)(24)(25). Remarkably, binding between ScbR2 and adpAp was observed (Fig.…”

Section: Significancementioning

confidence: 99%

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Angucyclines as signals modulate the behaviors of Streptomyces coelicolor

Wang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The angucycline antibiotic jadomycin B (JdB) produced by Streptomyces venezuelae has been found here to induce complex survival responses in Streptomyces coelicolor at subinhibitory concentration. The receptor for JdB was identified as a "pseudo" gammabutyrolactone receptor, ScbR2, which was shown to bind two previously unidentified target promoters, those of redD (redDp) and adpA (adpAp), thus directly regulating undecylprodigiosin (Red) production and morphological differentiation, respectively. Because AdpA also directly regulates the expression of redD, ScbR2, AdpA, and RedD together form a feed-forward loop controlling both differentiation and Red production phenotypes. Different signal strengths (i.e., JdB concentrations) were shown to induce the two different phenotypes by modulating the relative transcription levels of adpA vs. redD. The induction of morphological differentiation and endogenous antibiotic production by exogenous antibiotic exemplifies an important survival strategy more sophisticated than the induction of antibiotic resistance.

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ColonialDifferentiation in Streptomyces coelicolor Depends on Translation of a SpecificCodon within the adpA Gene

Cited by 72 publications

References 20 publications

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

Alternative oxygen‐dependent and oxygen‐independent ribonucleotide reductases in Streptomyces: cross‐regulation and physiological role in response to oxygen limitation

Angucyclines as signals modulate the behaviors of Streptomyces coelicolor

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