COLONIAL MORPHOLOGY AND VIRULENCE OF
            <i>PASTEURELLA PESTIS</i>

Eisler, Daniel M.; Kubik, Gayle; Preston, Herbert

doi:10.1128/jb.76.1.41-47.1958

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…A similar distinction between the smooth and nonsmooth forms of strain Poona was observed in a subsequent trial where L(-)arabinose was used. These reactions further substantiate the findings of Eisler et al (1958) that there are biochemical differences between the two variants. Table 2 summarizes the nonsmooth -k smooth dissociation induced in P. pestis strain Poona by several 5 carbon carbohydrates and other compounds.…”

Section: Description Of Reactantssupporting

confidence: 85%

DISSOCIATION IN PASTEURELLA PESTIS: INTERRELATIONS OF SMOOTH AND NONSMOOTH VARIANTS

1958

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The smooth and nonsmooth variants of Pasteurella pestis described by Eisler et al. (1958) have been investigated further. This study is concerned with establishing the heterogeneity of P. pestis strains and with factors influencing the nonsmooth ± smooth dissociations. METHODS AND MATERIALS Dissociation indices of Pasteurella pestis strains. Observations on viable cell numbers and the morphology of colonies isolated from suspensions of P. pestis strains Poona, 139L, 53,2 and 623 were made by the method described by Eisler et al. (1958). Generation time studies. Subcultures of smooth and nonsmooth colonial variants were made in 100-ml amounts of heart infusion broth (HIB, Difco) which was aerated by shaking at 230 strokes per minute on a reciprocal shaker at 28 C. Generation times were determined by the method of WVilson and Miles (1946) as applied to viable cell numbers only. A cell division factor of 2 was assumed since relative rather than absolute values were required. Serological comparisons. Serological comparisons of dissociants were made in Ouchterlony gel diffusion plates prepared as described by Crumpton and Davies (1956). The antigens were concentrated viable suspensions of smooth or nonsmooth colonies picked from the surfaces of I This work was sponsored by the Office of Naval Research, U. S. Navy, under a contract between the Office of Naval Research and the Regents of the University of California. Opinions expressed in this report are not to be construed as reflecting the view of the Navy Department or of the naval service at large (Article 1252, U. S. Navy Regulations, 1948). Reproduction in whole or in part is permitted for any purpose of the United States Government.

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Section: Description Of Reactantssupporting

confidence: 85%

DISSOCIATION IN PASTEURELLA PESTIS: INTERRELATIONS OF SMOOTH AND NONSMOOTH VARIANTS

1958

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“…An interesting observation was that all VW+ avirulent isolates exhibited rough colonial morphology upon incubation on MGOX at 37 C. Figure 1 illustrates a smooth, VW-avirulent colony and two rough, VW+ avirulent colonies obtained from the virulent nonglycerol-fermenting strain P23 (described below). These colonies were grown on MGOX for 48 hr at 37 C. In the case of the nonglycerol-fermenting strains, VW+ avirulent colonies were rough when grown at 26 C by the criterion of Eisler, Kubik, and Preston (1958), whereas the majority of virulent isolates formed smooth colonies at this temperature. Resistant isolates obtained from the glycerol-fermenting strain Siam and also from the glycerol-fermenting strain Yokohama (not included in this report) exhibited no correlation between smoothness and virulence when grown at 26 C.…”

Section: Resultsmentioning

confidence: 99%

GENOTYPIC ALTERATIONS ASSOCIATED WITH AVIRULENCE IN STREPTOMYCIN-RESISTANTPASTEURELLA PESTIS

Brubaker¹,

Surgalla²

1962

J Bacteriol

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typic alterations associated with avirulence in streptomycin-resistant Pasteurella pestis. J. Bacteriol. 84:615-624. 1962.-Avirulence in Pasteurella pestis, as indicated by the existence of a new genotype, was associated with the genetic loss of Ca++ dependence rather than with the usual concomitant loss of ability to produce virulence antigens. Strains of the new type exhibited all four of the known virulence factors of P. pestis but were avirulent for mice and guinea pigs (LD5o > 108 cells). In addition to producing virulence antigens, the new strains formed rough colonies at 37 C when grown on Caldeficient medium, as opposed to typical avirulent strains which formed smooth colonies on this medium and failed to produce virulence antigens. Growth of the new strains in a corresponding Ca++-deficient broth medium was slow, as compared with that of typical avirulent strains, but was more rapid than that of virulent strains. The new strains were obtained (i) at 26 C by selecting for resistance to streptomycin or (ii) at 37 C by isolation from rough colonies growing on a Ca++-deficient agar medium plated with either a virulent streptomycinsensitive or-resistant culture. Usually, isolates obtained at 26 C exhibited a marked reduction in ability to form colonies upon primary plating at 37 C. Of 108 streptomycin-resistant isolates obtained from six virulent strains, 20% were of the new type. In addition, five isolates were of reduced virulence (mouse LD5-104 cells) but differed in being Ca-+dependent; they possessed all of the known virulence properties. Certain streptomycin-resistant strains failed to give rise to avirulent colonies when plated on a Cal ldeficient agar medium containing streptomycin. The significance of this phenomenon,

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“…Colonial morphology was recorded after incubation for 2 days at 28 C followed by 6 to 8 days at room temperature. Criteria for smooth and nonsmooth colony characteristics are described by Eisler et al (1958).…”

Section: Methodsmentioning

confidence: 99%

DISSOCIATION IN PASTEURELLA PESTIS: IMMUNOLOGICAL COMPARISONS OF SMOOTH AND NONSMOOTH VARIANTS

1958

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COLONIAL MORPHOLOGY AND VIRULENCE OF PASTEURELLA PESTIS

Cited by 7 publications

References 7 publications

DISSOCIATION IN PASTEURELLA PESTIS: INTERRELATIONS OF SMOOTH AND NONSMOOTH VARIANTS

DISSOCIATION IN PASTEURELLA PESTIS: INTERRELATIONS OF SMOOTH AND NONSMOOTH VARIANTS

GENOTYPIC ALTERATIONS ASSOCIATED WITH AVIRULENCE IN STREPTOMYCIN-RESISTANTPASTEURELLA PESTIS

DISSOCIATION IN PASTEURELLA PESTIS: IMMUNOLOGICAL COMPARISONS OF SMOOTH AND NONSMOOTH VARIANTS

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