Colocalization of Neuronal NO Synthase with Urotensins I and II in the Caudal Neurosecretory Neurons and the Urophysis of the Teleost &lt;i&gt;Oreochromis niloticus&lt;/i&gt;

Cioni, Carla; Vito, Luana De

doi:10.1159/000006679

Cited by 9 publications

(3 citation statements)

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“…Biochemical and Western blot analyses also indicated that a constitutive NOS is expressed in fish caudal spinal cord, which is similar to mammalian nNOS [Cioni et al, 1998]. In the caudal neurosecretory neurons and the urophysis, nNOS is colocalized with caudal neurohormones, urotensins I and II [Cioni and De Vito, 2000]. These findings have suggested that NO is implicated in the modulation of hormone release in teleosts in a similar way to mammals [Cioni et al, 2002].…”

Section: Introductionmentioning

confidence: 76%

Expression of Nitric Oxide Synthase in the Preoptic-Hypothalamo-Hypophyseal System of the Teleost <i>Oreochromis niloticus</i>

et al. 2003

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In the present study, we have analyzed the expression of nitric oxide synthase (NOS) in the preoptic-hypothalamo-hypophyseal system of the teleost Oreochromis niloticus. The assay for enzyme activity demonstrated that a constitutive NOS activity is present both in soluble and particulate fractions of the homogenates of diencephalons. Western blot analysis using an antibody against the N-terminus of human nNOS revealed two bands both in the supernatant and in the pellet. One band co-migrates at approximately 150 kDa with that detected in the rat cerebellum homogenates and presumably corresponds to neuronal NOS (nNOS) of mammals. The additional band, which migrates at approximately 180 kDa, might be attributed to an alternatively spliced nNOS isoform. Using NADPH diaphorase (NADPHd) histochemistry in combination with NOS immunohistochemistry, nNOS expression has been detected in preoptic nuclei, hypophysiotrophic nuclei of the ventral hypothalamus, and the pituitary gland. Various degrees of dissociation of NADPHd activity and nNOS immunoreactivity have been detected that could be attributed to the expression of different subtypes of nNOS in the preoptic/hypothalamo/hypophysial system of tilapia. In this paper, we also investigated the colocalization of nNOS with arginine-vasotocin (AVT) by means of immunolabeling of consecutive sections. Results suggest that NO may be colocalized with AVT in a subpopulation of neurosecretory neurons. Present findings suggest that nitric oxide (NO) is implicated in the modulation of hormone release in teleosts in a similar way to mammals.

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Section: Introductionmentioning

confidence: 76%

Expression of Nitric Oxide Synthase in the Preoptic-Hypothalamo-Hypophyseal System of the Teleost <i>Oreochromis niloticus</i>

et al. 2003

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“…Neuronal NOS-like enzymes were localized in neuroendocrine neurons of the preoptichypothalamo-hypophysial and caudal neurosecretory system by means of NADPHd histochemistry and NOS immunohistochemistry [7,13], and direct evidence of colocalization of nNOS with arginine-vasotocine (AVT) or urotensins in these neurons was provided by means of double immunolabeling methods [6,12].…”

Section: Introductionmentioning

confidence: 99%

Partial cloning of neuronal nitric oxide synthase (nNOS) cDNA and regional distribution of nNOS mRNA in the central nervous system of the Nile tilapia Oreochromis niloticus

Bordieri

Patti

Miele

et al. 2005

Molecular Brain Research

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“…Since nos3 has not been identified in fishes, this evidence is probably due to the cross-reactivity of Nos1 with heterologous antibodies. The intracellular distribution of Nos1 molecules was analyzed for NOSI, NOSI/UI and NOSII/UII by single and double gold immunoelectron microscopy in order to compare the distribution of Nos1-like molecules with that of the urophysial peptides [197]. The subcellular localization of fish Nos1 correlates well with the biochemical data.…”

Section: Nitric Oxide In the Cnssmentioning

confidence: 99%

Nitric Oxide and the Neuroendocrine Control of the Osmotic Stress Response in Teleosts

Cioni

Angiulli

Toni

2019

IJMS

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The involvement of nitric oxide (NO) in the modulation of teleost osmoresponsive circuits is suggested by the facts that NO synthase enzymes are expressed in the neurosecretory systems and may be regulated by osmotic stimuli. The present paper is an overview on the research suggesting a role for NO in the central modulation of hormone release in the hypothalamo-neurohypophysial and the caudal neurosecretory systems of teleosts during the osmotic stress response. Active NOS enzymes are constitutively expressed by the magnocellular and parvocellular hypophysiotropic neurons and the caudal neurosecretory neurons of teleosts. Moreover, their expression may be regulated in response to the osmotic challenge. Available data suggests that the regulatory role of NO appeared early during vertebrate phylogeny and the neuroendocrine modulation by NO is conservative. Nonetheless, NO seems to have opposite effects in fish compared to mammals. Indeed, NO exerts excitatory effects on the electrical activity of the caudal neurosecretory neurons, influencing the amount of peptides released from the urophysis, while it inhibits hormone release from the magnocellular neurons in mammals.

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Colocalization of Neuronal NO Synthase with Urotensins I and II in the Caudal Neurosecretory Neurons and the Urophysis of the Teleost <i>Oreochromis niloticus</i>

Cited by 9 publications

References 78 publications

Expression of Nitric Oxide Synthase in the Preoptic-Hypothalamo-Hypophyseal System of the Teleost <i>Oreochromis niloticus</i>

Expression of Nitric Oxide Synthase in the Preoptic-Hypothalamo-Hypophyseal System of the Teleost <i>Oreochromis niloticus</i>

Partial cloning of neuronal nitric oxide synthase (nNOS) cDNA and regional distribution of nNOS mRNA in the central nervous system of the Nile tilapia Oreochromis niloticus

Nitric Oxide and the Neuroendocrine Control of the Osmotic Stress Response in Teleosts

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