A composite microlens array (MLA) with two cascaded guiding axes has been fabricated to achieve a large lateral separation of an object with different refractive indices or sizes. The MLA projects a composite pattern formed by its focal spots into a microchamber for optical sorting in a microscopic system. This approach enables passive, high power, efficient, and continuous microfluidic sorting without requiring complicated optical assembly. Separation of particles with different refractive indices to a lateral angle of 40° is experimentally demonstrated with moderate laser power.Optical tweezers that were invented by Ashkin et al. 1 are able to trap and manipulate colloidal particles or biological cells with a gradient force generated by a highly focused laser beam. In the development of optical trapping technology itself, it has been matured for applications ranging from simple trapping and manipulation to more sophisticated areas such as optical vortices, 2 optical actuator, 3 three-dimensional (3D) optical tweezers, 4 laser guidance, 5 creation of two-dimensional (2D)/3D colloidal crystals, and structures. 6 Among the applications, optical actuator and optical sorting are two key components that might give rise to all optical laboratory-on-a-chip and optofluidic devices. An all-optical sorting was reported that was based on an optical lattice created by multibeam interference particles that can be sorted by sizes or by refractive indices. 7 Unlike those approaches using fluorescence as labels for sorting, 8,9 the all-optical sorting method makes use of the natural physical attributes of particles, such as size or refractive index as a label for sorting; hence, it has the advantages of being continuous, noninteractive, and minimally invasive. In addition to the multi-interference, computer generated holograms 10,11 and phase contrast 12 are also used to generate optical lattice for sorting.The principle of all-optical sorting lies in the fact that the trajectories of particles may deviate when they pass through an optical potential landscape, and the trajectories of the particles are strongly dependent on their sizes and refractive indices. If relevant parameters and conditions are selected properly, the group of particles mixed with different sizes or refractive indices will be separated laterally. The lateral angle of the separation is a key