Colloidal Gold Filtrates as Metal Substrates for Surface-Enhanced Infrared Absorption Spectroscopy

Seelenbinder, John A.; Brown, Chris W.; Pivarnik, Philip E.; Rand, Arthur G.

doi:10.1021/ac981170l

Cited by 57 publications

(33 citation statements)

References 13 publications

(32 reference statements)

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“…Doseresponse curves were created for spiked buffer samples to assess the MAAB's overall performance and to provide a basis for comparison with other so-called rapid methods of detection. The LODs determined here for both 15-min and 1-h protocols (ϳ10 4 CFU/ml) compare favorably with those for several other antibody-based systems (LODs in the range of mid-10 3 to 10 5 CFU/ml), including piezoelectric sensors (4,33,43,45,59), fiber optic sensors (44,71,72), an interferometer (57), surface plasmon resonance-based sensors (9,56), and an electrochemical detector (22). ORIGEN, a system based on electrochemiluminescence, has proven to be the most sensitive rapid detection system, with a detection limit of 1,000 CFU/ml (70).…”

Section: Discussionsupporting

confidence: 63%

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Taitt

Shubin

Angel

et al. 2004

Appl Environ Microbiol

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The multianalyte array biosensor (MAAB) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. Rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of Salmonella enterica serovar Typhimurium, with a detection limit of 8 ؋ 10 4 CFU/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. S. enterica serovar Typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparation: sausage, cantaloupe, whole liquid egg, alfalfa sprouts, and chicken carcass rinse. Cross-reactivity tests were performed with Escherichia coli and Campylobacter jejuni. To determine whether the MAAB has potential as a screening tool for the diagnosis of asymptomatic Salmonella infection of poultry, chicken excretal samples from a private, noncommercial farm and from university poultry facilities were tested. While the private farm excreta gave rise to signals significantly above the buffer blanks, none of the university samples tested positive for S. enterica serovar Typhimurium without spiking; dose-response curves of spiked excretal samples from university-raised poultry gave limits of detection of 8 ؋ 10 3 CFU/g.

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Section: Discussionsupporting

confidence: 63%

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Taitt

Shubin

Angel

et al. 2004

Appl Environ Microbiol

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“…[24][25][26]44] Therefore, the increase in the IR absorption relative to the smooth surface is attributed to the SEIRS effect. However, as can be seen in Figure 3, the IR absorption at approximately 1234 cm À1 actually decreases with additional NP-deposition steps (although after the first layer of Au NPs, the amount of PDT increases with the number of Au NP depositions, see Scheme 1).…”

Section: Substrate Optimization and Application In Surface-enhanced Pmentioning

confidence: 99%

Self‐Assembled Au Nanoparticles as Substrates for Surface‐Enhanced Vibrational Spectroscopy: Optimization and Electrochemical Stability

Fan

Brolo

2008

ChemPhysChem

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Three-dimensional nanostructured metallic substrates for enhanced vibrational spectroscopy are fabricated by self-assembly. Nanostructures consisting of one to 20 depositions of 13 nm-diameter Au nanoparticles (NPs) on Au films are prepared and characterized by means of AFM and UV/Vis reflection-absorption spectroscopy. Surface-enhanced polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS) is observed from Au NPs modified by the probe molecule 4-hydroxythiophenol. The limitation of this kind of substrate for surface-enhanced PM-IRRAS is discussed. The surface-enhanced Raman scattering (SERS) from the same probe molecule is also observed and the effect of the number of Au-NP depositions on the SERS efficiency is studied. The SERS signal from the probe molecule maximizes after 11 Au-NP depositions, and the absolute SERS intensities from different batches are reproducible within 20%. In situ electrochemical SERS measurements show that these substrates are stable within the potential window between -800 and +200 mV (vs. Ag/AgCl/sat. Cl(-)).

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“…Normally, immunoassay requires selective quantitation of trace levels of target molecules in complex biosystem. However, former IR immunoassays were ex situ and showed poor detection limit which extremely restricted the application [72,73]. In 2012, ATR-SEIRAS technique was first introduced into immunoassay by our group [74].…”

Section: Immunoassaymentioning

confidence: 99%

Attenuated Total Reflection Surface-Enhanced Infrared Absorption Spectroscopy: a Powerful Technique for Bioanalysis

Zhang

et al. 2017

J. Anal. Test.

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Attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) has recently been proven to be a powerful tool for bioanalysis. It enables in situ and in real-time observation of dynamic processes occurring on specific interface, revealing rich structural and functional information of biomolecules at sub monolayer level. The aim of this general review was to give an overview of the cutting edge applications of ATR-SEIRAS. We start with description of the basic configuration of the standard ATR-SEIRAS platform. The enhanced mechanisms and methods to fabricate enhanced substrates are then presented. We discuss the recent developments, challenges and applications of ATR-SEIRAS in bioanalysis, mainly focusing on DNA analysis, protein behavior and cell properties. Finally, further development of the ATR-SEIRAS technique with enhanced sensitivity, improved time and spatial resolutions will be prospected.

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Colloidal Gold Filtrates as Metal Substrates for Surface-Enhanced Infrared Absorption Spectroscopy

Cited by 57 publications

References 13 publications

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Self‐Assembled Au Nanoparticles as Substrates for Surface‐Enhanced Vibrational Spectroscopy: Optimization and Electrochemical Stability

Attenuated Total Reflection Surface-Enhanced Infrared Absorption Spectroscopy: a Powerful Technique for Bioanalysis

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