Colloidal gold and fluorescent immunochromatographic test strips for canine parvovirus detection

Zhang, Chengqi; Wan, Ying; Shi, Zhengwang; Luo, Juncong; Li, Hongye; Li, Shuangshuang; Li, Yun-Zhen; Dai, Xinyu; Bai, Xiaoshuan; Tian, Hong; Zheng, Haixue

doi:10.1007/s00253-023-12604-2

Cited by 2 publications

(1 citation statement)

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“…The indirect enzyme-linked immunosorbent assay (ELISA) is a widely employed technique for the detection of antibodies and is renowned for its rapidity, high sensitivity, exceptional specificity, and straightforward methodology [ 19 ]. Based on these attributes, we developed an indirect ELISA protocol for quantifying GnRH-MBP antibodies in rat serum.…”

Section: Introductionmentioning

confidence: 99%

Effect of GnRH Active Immunisation on Reproductive Performance of Male Sprague Dawley Rats

Zhang,

Li,

et al. 2024

IJMS

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To investigate the effect of active immunisation with gonadotropin-releasing hormone (GnRH) on the reproductive function in male Sprague Dawley (SD) rats, 24 42-day-old rats were randomly assigned to treatment with GnRH6-MAP, GnRH-OVA, a surgical castration group, and a blank control group. Each rat in the treatment groups was intramuscularly injected at 6, 8, and 10 weeks of age. The serum concentrations of testosterone (T), follicle-stimulating hormone (FSH), luteinising hormone (LH), and anti-GnRH antibodies were determined using enzyme-linked immunosorbent assays. The results showed that active immunisation with recombinant GnRH6-MBP and GnRH-OVA significantly increased the serum levels of anti-GnRH antibodies and reduced the serum concentrations of testosterone compared to the black control. Eight weeks after immunisation, the rats’ testes were surgically removed for morphological evaluation, showing atrophy of the convoluted vasculature, relative emptying of the lumen, and insignificant differentiation of spermatogonial cells, which were increased in weight and volume compared with the blank control group. These findings indicated that active immunisation with GnRH can lead to testicular atrophy and reduce gonadal hormone concentrations, suggesting that GnRH is a highly effective immunogen.

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Section: Introductionmentioning

confidence: 99%