Colloidal effect of albumin on the placental lactogen and chorionic gonadotrophin releases from human term placental explants

Lambot, Nathalie; Lebrun, Philippe; Cirelli, Nadia; Vanbellinghen, Anne-Marie; Delogne-Desnoeck, J.; Graff, G. L. A.; Meuris, Sylvain

doi:10.1016/j.bbrc.2004.01.062

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…In our study, the impact of the flow system on placental tissue viability and structural integrity was analyzed using unbiased morphological and biochemical parameters. Previous studies reported superfused placental explants at a moist cellulose filter inserted in a perfusion chamber [ 29 , 30 , 31 ]; however, they revealed restricted tissue viability [ 20 ]. To our knowledge, this study is the first proving the benefits of culturing tissues under flow in terms of biochemical and structural viability.…”

Section: Discussionmentioning

confidence: 99%

Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation

Kupper

Pritz

Siwetz

et al. 2021

IJMS

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During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. However, static placental villous explant culture dramatically differs from the in vivo situation. Thus, we established a flow culture system for placental villous explants and compared commonly used static cultured tissue to flow cultured tissue using transmission and scanning electron microscopy, immunohistochemistry, and lactate dehydrogenase (LDH) and human chorionic gonadotropin (hCG) measurements. The data revealed a better structural and biochemical integrity of flow cultured tissue compared to static cultured tissue. Thus, this new flow system can be used to simulate the blood flow from the mother to the placenta and back in the most native-like in vitro system so far and thus can enable novel study designs.

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