Colloidal Crystal Beads as Supports for Biomolecular Screening

Zhao, Xiangwei; Cao, Yun; Ito, Fuyumi; Chen, Haihua; Nagai, Keiji; Zhao, Yuhua; Gu, Zhongze

doi:10.1002/anie.200601302

Cited by 143 publications

(104 citation statements)

References 25 publications

(17 reference statements)

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“…22) using a co-flow PDMS chip and a tubing/pipette tip device. Similar results were reported by Gu et al later on [236]. In both cases, nano-sized seed particles in aqueous suspension droplets were emulsified in oil and the assembly was realized via the removal of water.…”

Section: Examples Of Microfluidic Particle Productionsupporting

confidence: 85%

See 1 more Smart Citation

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Gökmen

Prez

2012

Progress in Polymer Science

446

309

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Section: Examples Of Microfluidic Particle Productionsupporting

confidence: 85%

“…These approaches are actually multistage heterophase polymerizations in which the second stage is microfluidics. The first stages, namely seed preparation, were either dispersion [234] or emulsifier-free emulsion [235][236] polymerizations. …”

Section: Examples Of Microfluidic Particle Productionmentioning

confidence: 99%

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Gökmen

Prez

2012

Progress in Polymer Science

446

309

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“…Zhao et al [86] demonstrated biological screening using these photonic microparticles prepared to have three diff erent colors. Red, green and blue photonic microparticles were prepared from monodisperse polymethylmethacrylate particles with diameters of 250 nm, 220 nm and 210 nm, and human, mouse and rabbit immunoglobulin G (IgG), respectively, were immobilized on the surfaces of photonic microparticles.…”

Section: Biological Toolsmentioning

confidence: 99%

Self-assembled colloidal structures for photonics

et al. 2011

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A s dielectric structures with a submicrometer length scale can interact strongly with light, various remarkable optical responses can be designed and tailored depending on the types and parameters of their structures. Over the past few decades, the unusual optical properties of the periodic dielectric structures called photonic crystals have been investigated intensively [1]. Many research groups have endeavored to engineer the optical properties of photonic crystals, including photonic bandgaps, 'slow' photons, negative refraction and other properties, or to use them in practical applications. Two-dimensional (2D) structures, which are mostly prepared by conventional lithographic processes, were demonstrated initially, in which total internal refl ections were adopted for confi ning the light in a nonperiodic third direction, and their use has been investigated in some limited applications [2]. Th ree-dimensional (3D) structures have also been investigated intensively because of their complete photonic bandgaps in certain structures, a critical property for controlling light in 3D space. Research on such structures has been supported by the recent development of facile fabrication methods, including the selfassembly of simple monodisperse particles, also known as colloidal self-assembly [3], block copolymer self-assembly [4], the auto-cloning process [5] and holographic lithography [6]. Of these methods, colloidal self-assembly is the most promising for the low-cost production of 2D and 3D photonic crystals over large areas or with various shapes [7,8]. Schematic diagrams of the basic colloidal crystal structure along with inverse and short-range-ordered scattering structures for photonic applications are shown in Figure 1. Disordered dielectric structures of monodisperse particles called photonic glasses have begun to be investigated as another class of photonic nanostructures that can manifest some unusual optical phenomena such as random lasing, strong light localization and long-range intensity correlations. In this review article, we describe self-assembled colloidal photonic nanostructures in brief and summarize recent achievements in the fi eld of colloidal photonic nanostructures and their applications. Fabrication of photonic nanostructures by colloidal assembly Colloidal crystalsSince Vanderhoff 's serendipitous discovery of a synthetic method for preparing monodisperse polymer colloids [9], the method has been extended to the preparation of a variety of polymeric colloids and also to the processing of inorganic colloidal particles such as silica, titania and iron oxide. As long as particles are stable in liquid and their size distribution is suffi ciently narrow, they can be crystallized in a facecentered cubic (fcc) lattice by increasing their volume fraction through any concentration process, such as controlled evaporation, sedimentation or fi ltration. In general, the interparticle forces can be described by summing over the various potentials from diff erent origins, including intermolecular for...

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“…To achieve multiplexing, it is necessary to code the beads to recognize which bead corresponds to which analyte. Various methods such as intensity of fluorescence, (12) multicolor fluorescence, (14) shape, (16) colloidal crystal, (17) metallic barcoding, (18) and surface-enhanced Raman scattering (19) have been proposed as bead-coding methods. Among these, fluorescence-based encoding is most commonly used.…”

Section: Introductionmentioning

confidence: 99%

Development of a Bead-Based Multiplexed Immunoassay Using Image Cytometric Analysis

2017

Sensors and Materials

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Keywords: multiplexed immunoassay image cytometry, bead-based assayIn this paper, we present the demonstration of a size-encoded bead-based multiplexed immunoassay with image cytometric decoding and readout. Antibodies were immobilized on polystyrene microspheres by physical adsorption and employed for the demonstration of this duplexed immunoassay. First, we carried out a duplexed immunoassay with a mouse and rat IgG detection system and discussed the problem of cross-reactivity inherent to these immunoreaction systems. We then carried out a duplexed immunoassay with a mouse IgG and human albumin detection system, which was less cross-reactive. We subsequently demonstrated the application of size-encoded image cytometric decoding and the detection of mouse IgG and human albumin using this duplexed immunoassay system. BackgroundImmunoassays are one type of analytical method, which are widely applied for the detection and quantitation of proteins, owing to their simplicity and high sensitivity.(1-4) Recent progress in proteomics has revealed correlations among disease, bioactivity, and molecular information, and this has strongly motivated the clinical application of multiplexed protein assay methods.(5-7)Protein microarrays, which are based on specific antibodies, are the ideal tool for multiplexed immunoassays. (8,9) A spot of antibody is printed locally using microprinting technology for the application of conventional, but multiplexed, immunoreactions. Therefore, antibody printing is becoming the main technical difficulty in carrying out these assays. Moreover, the reaction kinetics are relatively slow owing to the lengthy diffusion of molecules, because the reaction is carried out on a planar surface. (10,11) As an alternative technique, bead-based multiplexed immunoassays have been intensively researched to overcome the drawbacks of microarray technology. (12)(13)(14)(15) In the case of beadbased immunoassays, multiplexing is achieved by preparing multiple antibody-immobilized beads corresponding to each analyte, and thus multiplexed immunoreactions can be implemented

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Colloidal Crystal Beads as Supports for Biomolecular Screening

Cited by 143 publications

References 25 publications

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Self-assembled colloidal structures for photonics

Development of a Bead-Based Multiplexed Immunoassay Using Image Cytometric Analysis

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