Colloidal CdSe and ZnSe/Mn quantum dots: Their cytotoxicity and effects on cell morphology

Дробинцева, А О; Matyushkin, L. B.; Александрова, О.В.; Дробинцев, Павел; Kvetnoy, Igor M.; Mazing, D. S.; Мошников, В. А.; Polyakova, V. O.; Мusikhin, Sergey F.

doi:10.1016/j.spjpm.2015.11.003

Cited by 7 publications

(10 citation statements)

References 10 publications

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“…Herewith the acidity of the solution does not affect the stability of the QDs, since the fluorescence was observed in an hour in all three samples with pH values equal to 8.0, 7.5, and 5.0, respectively. This result is consistent with our previous publication [32].…”

Section: Stabilitysupporting

confidence: 94%

Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles

Matyushkin¹,

Александрова²,

Дробинцева³

et al. 2020

Fluorescence Methods for Investigation of Living Cells and Microorganisms

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Clinical experience indicates that enhanced level of heat shock protein 70 (Hsp70) and p53 correlates with poor prognosis due to malignant cell overexpression of these proteins in tumor progression. Cadmium selenide quantum dots (QDs) were synthesized in aqueous solution using mercaptopropionic acid and L-cysteine (L-Cys) as ligands. They were conjugated with a monoclonal antibody (Ab) to p53 and cmHp70.1 to Hsp70 for detection of cancer cell apoptosis that was demonstrated in the experiment by fluorescent confocal microscopy both for breast carcinoma cells and for thyroid tissue. It is shown that in comparison with organic dyes, quantum dots have superior photostability of tracking apoptosis in cancer cells for longer time.

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Section: Stabilitysupporting

confidence: 94%

Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles

Matyushkin¹,

Александрова²,

Дробинцева³

et al. 2020

Fluorescence Methods for Investigation of Living Cells and Microorganisms

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“…In contrast, MPA-coated Mn-doped ZnSe QDs did not show a statistically significant reduction in cell viability at any concentration tested (Figure 2b). These results are consistent with previous reports of minimal toxicity for Mn-doped QDs [25,27,30]. Despite differences in composition (i.e., ZnSe vs. ZnS or their combination) and synthesis routes, these studies and our work confirm lower toxicity for Mn-doped QDs compared to conventional CdSe/ZnSe QDs when ligand exchange is used to form a thin coating on particle surfaces.…”

Section: Qd Effects On Cell Viabilitysupporting

confidence: 93%

“…Toxicity resulting from composition can be addressed through the use of cadmiumfree QDs [20,21]. Several alternative compositions are now available, such as InP- [22,23] and Mn-doped ZnSe-or ZnS-based compositions [24][25][26][27][28][29][30][31]. Mn-doped QDs are particularly interesting because they emit photoluminescence from their doped centres rather than delocalised surface states, increasing photostability against surface chemical-or photooxidation [32].…”

Section: Introductionmentioning

confidence: 99%

Effect of Micelle Encapsulation on Toxicity of CdSe/ZnS and Mn-Doped ZnSe Quantum Dots

et al. 2021

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The optical properties of quantum dots (QD) make them excellent candidates for bioimaging, biosensing, and therapeutic applications. However, conventional QDs are comprised of heavy metals (e.g., cadmium) that pose toxicity challenges in biological systems. Synthesising QDs without heavy metals or introducing thick surface coatings, e.g., by encapsulation in micelles, can reduce toxicity. Here, we examined the toxicity of micelle encapsulated tetrapod-shaped Mn-doped ZnSe QDs, comparing them to 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnSe QDs prepared by ligand exchange and commercial CdSe/ZnS QD systems that were either capped with MPA or encapsulated in micelles. HepG2 cell treatment with MPA-coated CdSe/ZnS QDs resulted in a dose-dependent reduction of viability (MTT assay, treatment at 0–25 μg/mL). Surprisingly, no reactive oxygen species (ROS) or apoptotic signaling was observed, despite evidence of apoptotic behavior in flow cytometry. CdSe/ZnS QD micelles showed minimal toxicity at doses up to 25 μg/mL, suggesting that thicker protective polymer layers reduce cytotoxicity. Despite their shape, neither MPA- nor micelle-coated Mn-doped ZnSe QDs displayed a statistically significant toxicity response over the doses investigated, suggesting these materials as good candidates for bioimaging applications.

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“…The principle of this method is that the fluorescence emission of tryptophan can be detected in the visible range 450–460 nm upon excitation in the ultraviolet region (330 nm), which is appropriate to avoid spectral overlap of the SPR band of AuNPs located at 530 nm . Therefore, this simple, rapid, and sensitive imaging strategy may provide a new insight for probing their uptake, biocompatibility, and toxicity by a label-free fluorescence method. − Most of the QDs having excellent photostability are appropriate for in vitro and in vivo imaging; however, they are composed of heavy metals that are toxic, making them unsuitable in developing imaging applications. , Therefore, colloidal AuNPs can be an alternative in developing imaging applications due to ease of synthesis, colloidal stability, facile conjugation procedure, and excellent biocompatibility. , …”

Section: Resultsmentioning

confidence: 99%

Temperature Dependent Synthesis of Tryptophan-Functionalized Gold Nanoparticles and Their Application in Imaging Human Neuronal Cells

Kim

Shinde

et al. 2017

ACS Sustainable Chem. Eng.

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The growth kinetics and temporal evolution of the UV–vis spectrum of gold nanoparticles (AuNPs) by following tryptophan reduction in different temperature conditions were studied systematically. The results revealed the productivity and overall reaction mechanism were mostly determined by the temperature, which was in turn affected by the concentration of tryptophan. Two considerably different reaction pathways were observed. The first pathway occurred at ambient temperature (35 °C) and consisted of three corresponding steps: nucleation, growth, and oriented attachment. The second pathway occurred above the ambient temperature (45, 65, and 95 °C) and was responsible for the well-known nucleation–growth route. The second pathway was used to develop a facile synthetic route for the preparation of functionalized AuNPs with the size about 20 nm. Consequently, the stability and functionalization of the AuNPs were demonstrated using dilution studies, zeta potential, and FTIR measurements. The imaging of human neuronal (SH-SY5Y) cells showed that the fluorescent signal from the tryptophan-functionalized AuNPs was significantly brighter than that from autofluorescence of the cells. The strong signal, resistance to photobleaching, excellent stability, ease of synthesis, simplicity of functionalization, and biocompatibility make AuNPs an attractive option for imaging and biomedical applications.

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Colloidal CdSe and ZnSe/Mn quantum dots: Their cytotoxicity and effects on cell morphology

Cited by 7 publications

References 10 publications

Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles

Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles

Effect of Micelle Encapsulation on Toxicity of CdSe/ZnS and Mn-Doped ZnSe Quantum Dots

Temperature Dependent Synthesis of Tryptophan-Functionalized Gold Nanoparticles and Their Application in Imaging Human Neuronal Cells

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