Colloid supported lipid bilayers for self-assembly

Rinaldin, Melissa; Verweij, Ruben W.; Chakraborty, Indrani; Kraft, Daniela J.

doi:10.1039/c8sm01661e

Cited by 30 publications

(70 citation statements)

References 73 publications

(143 reference statements)

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“…The CSLBs consisting of 2.12 μm silica particles were prepared as described in our recent work [43]. Briefly, the particles were coated with a fluid lipid bilayer by deposition of small unilamellar vesicles consisting of 98.8 mol% DOPC…”

Section: A Experimentsmentioning

confidence: 99%

Flexibility-induced effects in the Brownian motion of colloidal trimers

Verweij

Moerman

Ligthart

et al. 2020

Phys. Rev. Research

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Shape changes resulting from segmental flexibility are ubiquitous in molecular and biological systems, and are expected to affect both the diffusive motion and (biological) function of dispersed objects. The recent development of colloidal structures with freely jointed bonds have now made a direct experimental investigation of diffusive shape-changing objects possible. Here, we show the effect of segmental flexibility on the simplest possible model system, a freely jointed cluster of three spherical particles, and validate long-standing theoretical predictions. We find that, in addition to the rotational diffusion time, an analogous conformational diffusion time governs the relaxation of the diffusive motion, unique to flexible assemblies, and that their translational diffusivity differs by a small but measurable amount. We also uncovered a Brownian quasiscallop mode, where diffusive motion is coupled to Brownian shape changes. Our findings could have implications for molecular and biological systems where diffusion plays an important role, such as functional site availability in lock-and-key protein interactions.

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Section: A Experimentsmentioning

confidence: 99%

Flexibility-induced effects in the Brownian motion of colloidal trimers

Verweij

Moerman

Ligthart

et al. 2020

Phys. Rev. Research

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“…For the experiments with cubic particles, we heated the sample to 450 1C to burn the polymers used for the stabilisation of the cubic colloids from the surface, as shown in ref. 34. In some experiments with symmetric and asymmetric dumbbells and spheres, we also burnt the particles at 450 1C to remove the inner polymer network of polystyrene and TPM.…”

Section: B Substrates and Colloidsmentioning

confidence: 99%

Lipid exchange enhances geometric pinning in multicomponent membranes on patterned substrates

et al. 2020

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“…If it is possible to satisfy the three inequalities, Eqs. (10) and (11), simultaneously, for a given value of r 0 , t, and L¯k, then our approach is experimentally feasible and can be used to measure Δk. Experiments are assumed to be carried out near room temperature (T ≈ 300 K) and the typical values for the line tension span σ ∼ 0.1-3.0 pN [33].…”

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confidence: 99%

“…In vitro such a transition is often controlled by decreasing the temperature below some critical value. In the present work we analyze two-phase membranes supported on preengineered substrate surfaces, such as have recently been developed [9,10].…”

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confidence: 99%

“…While the energy equation (1) can describe the shape of phase-separated freestanding membranes, such as multicomponent vesicles [20,23], it also applies to the case of curved supported lipid bilayers, where the membrane lies on a rigid substrate of nontrivial curvature, while preserving its liquid nature [24][25][26]. These experimental techniques have been recently extended to a wide variety of shapes with both open and closed surfaces in [9,10]. Thus, supporting a membrane using a preengineered surface with arbitrary shape is therefore now a realistic proposal [27].…”

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Measuring Gaussian Rigidity Using Curved Substrates

et al. 2020

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The Gaussian (saddle splay) rigidity of fluid membranes controls their equilibrium topology but is notoriously difficult to measure. In lipid mixtures, typical of living cells, linear interfaces separate liquid ordered (LO) from liquid disordered (LD) bilayer phases at subcritical temperatures. Here, we consider such membranes supported by curved substrates that thereby control the membrane curvatures. We show how spectral analysis of the fluctuations of the LO-LD interface provides a novel way of measuring the difference in Gaussian rigidity between the two phases. We provide a number of conditions for such interface fluctuations to be both experimentally measurable and sufficiently sensitive to the value of the Gaussian rigidity, while remaining in the perturbative regime of our analysis.

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Colloid supported lipid bilayers for self-assembly

Abstract: Careful tuning of the experimental parameters involved in preparing colloid supported lipid bilayers enables the self-assembly of flexible structures.

Cited by 30 publications

References 73 publications

Flexibility-induced effects in the Brownian motion of colloidal trimers

Flexibility-induced effects in the Brownian motion of colloidal trimers

Lipid exchange enhances geometric pinning in multicomponent membranes on patterned substrates

Measuring Gaussian Rigidity Using Curved Substrates

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