Collision induced dissociation-based characterization of nucleotide peptides: Fragmentation patterns of microcin C7–C51, an antimicrobial peptide produced by <i>Escherichia coli</i>

Petit, Vanessa; Zirah, Séverine; Rebuffat, Sylvie; Tabet, Jean‐Claude

doi:10.1016/j.jasms.2008.04.020

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…Electrospray ionization (ESI) for analysis of crosslinks was employed at a later point, connecting liquid chromatography with triple quadrupole/linear ion trap (Q-trap [97,99]), quadrupole time-of-flight (Q-ToF [83][84][85][86]) or Orbitrap instruments [100]. Overall, on-line LC-ESI-MS requires much smaller sample quantities compared with off-line LC coupled with MALDI-MS and yields better sequence information.…”

Section: 4mentioning

confidence: 99%

“…Especially the low-mass cut-off is problematic, as this prevents the detection of marker ions (see below and Table 1), immonium ions and ions from very short peptide sequences. However, Petit et al demonstrated that MSMS in positive-and negative-ion mode can be applied to characterize an isolated microcin, comprising only seven amino acids and with its C-terminal amino acid covalently linked to a phosphoramidate group substituted with an adenosine and a propylamine [100]. Orbitrap instruments provide an additional mode of CID fragmentation, higher-energy C-trap dissociation (HCD).…”

Section: 4mentioning

confidence: 99%

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Investigation of protein–RNA interactions by mass spectrometry—Techniques and applications

Schmidt

Kramer

Urlaub

2012

Journal of Proteomics

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Section: 4mentioning

confidence: 99%

Section: 4mentioning

confidence: 99%

Investigation of protein–RNA interactions by mass spectrometry—Techniques and applications

Schmidt

Kramer

Urlaub

2012

Journal of Proteomics

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“…A high‐performance liquid chromatography (HPLC) method coupled to tandem mass spectrometry (MS/MS) has been established as a critical and powerful analytical tool for protein analysis, nucleic acid analysis, molecular biology and structural characterization of macromolecules as well as in discovery of biomarkers for a variety of diseases 5–15. The present study reports the application of HPLC/nESI‐MS/MS (nESI: nano‐electrospray ionization) in revealing new structural characteristics of an acid PLA 2 isolated from Chinese Agkistrodon blomhoffii Ussurensis snake venom (ABUSV).…”

mentioning

confidence: 99%

Electrospray ionization mass spectrometry as a critical tool for revealing new properties of snake venom phospholipase A₂

Liu

Zhang

et al. 2009

Rapid Comm Mass Spectrometry

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Results from high-performance liquid chromatography/nano-electrospray ionization tandem mass spectrometry (HPLC/nESI-MS/MS) coupled to two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D SDS-PAGE) indicated that the monomer and dimer of phospholipase A 2 (PLA 2 ) coexisted in crude Chinese Agkistrodon blomhoffii Ussurensis snake venom (ABUSV). Then, an acidic PLA 2 with the accurate molecular mass of 13979.6 Da was purified from ABUSV (mo-ABUSV-aPLA 2 ). MS/MS-derived peptides from ABUSV-aPLA 2 were compared with other homologous snake venom PLA 2 s, which in turn showed that ABUSV-aPLA 2 is a novel snake venom PLA 2 . Meanwhile, the ABUSV-aPLA 2 dimer (di-ABUSV-aPLA 2 ) was also obtained. MS/MS analysis identified the same peptides from di-ABUSV-aPLA 2 as from mo-ABUSV-aPLA 2 , which indicates that di-ABUSV-aPLA 2 is a homodimer. One Ca 2R ion is contained per ABUSV-aPLA 2 . The Ca 2R ion is critical for both the hydrolytic activity and the structure of ABUSV-aPLA 2 . Pro-Q Emerald and Pro-Q Diamond specific glycoprotein and phosphoprotein staining combined with MS/ MS analysis indicated that the ABUSV-aPLA 2 is both a glycoprotein and a phosphoprotein, which to our knowledge is the first such report for a snake venom PLA 2 and thus provides new threads for the study of the functions and structures of snake venom PLA 2 s. One phosphorylation site and the size of the glycan chain are determined by using HPLC/nESI-MS/MS and matrix-assisted laser desorption/ ionization time-of-flight (MALDI-TOF) MS. The delicate utilization of ESI-MS can exert tremendous impact on protein sciences.

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Prompt and Slow Electron‐Detachment‐Dissociation/Electron‐Photodetachment‐Dissociation of a 21‐Mer Peptide

Pérot-Taillandier

Zirah

Enjalbert

et al. 2012

Chemistry A European J

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Electron detachment dissociation (EDD) and electron photodetachment dissociation (EPD) are relatively new dissociation methods that involve electron detachment followed by radical-driven dissociation from multiply deprotonated species. EDD yields prompt dissociation whereas only electron detachment is obtained by EPD; subsequent vibrational activation of the charge-reduced radical anion is required to obtain the product ions. Herein, the fragmentation patterns that were obtained by EDD and by vibrational activation of the charge-reduced radical anions that were produced through EDD or EPD (activated-EDD and activated-EPD) were compared. The observed differences were related to the dissociation kinetics and/or the contribution of electron-induced dissociation (EID). Time-resolved double-resonance experiments were performed to measure the dissociation rate constants of the EDD product ions. Differences in the formation kinetics were revealed between the classical EDD/EPD 'a(⋅)(i)/''x(j) complementary ions and some 'a(⋅)(i)/c(i)/'''z(⋅)(j) product ions, which were produced with slower dissociation rate constants, owing to the presence of specific neighbouring side chains. A new fragmentation pathway is proposed for the formation of the slow-kinetics 'a(⋅)(i) ions.

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Collision induced dissociation-based characterization of nucleotide peptides: Fragmentation patterns of microcin C7–C51, an antimicrobial peptide produced by Escherichia coli

Cited by 7 publications

References 34 publications

Investigation of protein–RNA interactions by mass spectrometry—Techniques and applications

Investigation of protein–RNA interactions by mass spectrometry—Techniques and applications

Electrospray ionization mass spectrometry as a critical tool for revealing new properties of snake venom phospholipase A₂

Prompt and Slow Electron‐Detachment‐Dissociation/Electron‐Photodetachment‐Dissociation of a 21‐Mer Peptide

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Collision induced dissociation-based characterization of nucleotide peptides: Fragmentation patterns of microcin C7–C51, an antimicrobial peptide produced by Escherichia coli

Cited by 7 publications

References 34 publications

Investigation of protein–RNA interactions by mass spectrometry—Techniques and applications

Investigation of protein–RNA interactions by mass spectrometry—Techniques and applications

Electrospray ionization mass spectrometry as a critical tool for revealing new properties of snake venom phospholipase A2

Prompt and Slow Electron‐Detachment‐Dissociation/Electron‐Photodetachment‐Dissociation of a 21‐Mer Peptide

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Product

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Electrospray ionization mass spectrometry as a critical tool for revealing new properties of snake venom phospholipase A₂