Colletotrichum species causing anthracnose on lima bean in Brazil

Sousa, E.; Silva, Janaíne Rossane Araújo; Assunção, Iraíldes Pereira; Melo, Maruzanete Pereira de; Feijó, Frederico Monteiro; Matos, Kedma da Silva; Lima, Gaus Silvestre de Andrade; Beserra, José Evando Aguiar

doi:10.1007/s40858-017-0182-0

Cited by 20 publications

(20 citation statements)

References 34 publications

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“…C. cliviae was originally described without mention to the perfect stage (Yang et al 2009), but occurrence of the sexual stage was later reported on Orchidaceae in China by Yang et al (2011) (asci 60-82 μm × 9.5-14 μm, ascospores 12.5-21.5 μm × 5-7.5 μm). In addition, just as this paper was being submitted to publication, Sousa et al (2017) have reported C. cliviae causing anthracnose on lima bean (Phaseolus lunatus L.), in the warm Brazilian states of Piauí and Alagoas, and also recorded formation of the sexual stage. Reported metrics for ascospores in that paper were 7.1-13.4 μm × 1.8-6.8 μm.…”

Section: Resultsmentioning

confidence: 92%

Biology, pathogenicity, and haplotype analyses of Colletotrichum cliviae: a novel soybean anthracnose agent in warm tropical areas

Dias

Fonseca

Dias-Neto

et al. 2018

Trop. plant pathol.

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Soybean anthracnose is a complex disease, comprising isolates of Colletotrichum truncatum and at least six other species. In Brazil, C. cliviae was recently reported as a novel causal agent of soybean anthracnose but very little information is available about the C. cliviae-soybean pathosystem. Here we report the occurrence of C. cliviae in commercial soybean fields in the Tocantins State, TO (Northern Brazil). We also describe its most distinctive morphological, biological, cultural, and pathogenic aspects, particularly in contrast with C. truncatum (the prevalent anthracnose-causing species in Brazil), including symptoms, production of sexual structures, host range, seed infection and aggressiveness to soybean cultivars. The results indicated that the involvement of C. cliviae as an anthracnose-inducing species might impact current control strategies, including crop rotation systems and the replacement of susceptible cultivars. Studies were also carried out to clarify if the Brazilian soybean-infecting C. cliviae isolates were introduced into the country via contaminated seeds or if populations are endemic. The haplotype diversity of Brazilian as well as of a worldwide collection of C. cliviae isolates (available at the GenBank) was investigated based upon polymorphisms in three genomic regions (β-tubulin 2, glyceraldehyde-3-phosphate dehydrogenase, and actin). Nine haplotypes were identified among worldwide C. cliviae isolates from six different hosts. The Brazilian soybean isolates were placed into the closely related haplotypes 5 (composed by isolates from Mato Grosso State) and 6 (which included the isolate from TO), which were discriminated from each other by one single nucleotide polymorphism. Haplotype 6 group was also composed by a Chinese isolate from Camelia sinensis. The results suggest that this cosmopolitan fungal species is more likely endemic to Brazil where it has been reported in association with plant species across distinct botanical families. Therefore, the recent outbreaks of C. cliviae isolates on soybeans can be explained by either previous misdiagnosis or they may represent a recent shift/adaptation of this fungus to this legume crop. Thus far, the geographic occurrence of C. cliviae on soybeans is restricted to northern (warm) tropical regions of Brazil. The present report adds novel and relevant information with potential for the control of anthracnose caused by C. cliviae isolates in Brazil, and possibly in warm regions elsewhere.

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Section: Resultsmentioning

confidence: 92%

Biology, pathogenicity, and haplotype analyses of Colletotrichum cliviae: a novel soybean anthracnose agent in warm tropical areas

Dias

Fonseca

Dias-Neto

et al. 2018

Trop. plant pathol.

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“…Colletotrichum cliviae Yan L. Yang et al. was reported as an anthracnose pathogen of Arundina graminifolia , Capsicum sp., Clivia miniata , Cymbidium hookerianum and Zamioculas zamiifolia in China ( Diao et al., 2017 , Yang et al., 2009 , Yang et al., 2011 , Zhou and Li, 2017 ) and of soybean, lima bean and grapevine in Brazil ( Santos et al., 2018 , Sousa et al., 2018 , Barbieri et al., 2017 ), on Cattleya sp. , Calamus thwaitesii , Phaseolus sp.…”

Section: Resultsmentioning

confidence: 99%

“…were revealed to be C. plurivorum as well. Strains from studies on lima beans ( Phaseolus lunatus ) in Brazil ( Sousa et al., 2018 , Cavalcante et al., 2018 ) probably also belong to this species, based on sequence comparisons of GAPDH and ACT sequences ( TUB2 was mixed-up with a C. truncatum strain) or a multilocus phylogeny, respectively. Many species have been reported or were described from these hosts ( Farr & Rossman 2017 , this study).…”

Section: Resultsmentioning

confidence: 99%

The Colletotrichum dracaenophilum, C. magnum and C. orchidearum species complexes

Damm

Sato

Alizadeh

et al. 2019

Studies in Mycology

204

203

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Although Glomerella glycines, Colletotrichum magnum and C. orchidearum are known as causal agents of anthracnose of soybean, Cucurbitaceae and Orchidaceae, respectively, their taxonomy remains unresolved. In preliminary analyses based on ITS, strains of these species appear basal in Colletotrichum phylogenies, clustering close to C. cliviae, C. brevisporum and other recently described species from tropical or subtropical regions. Phylogenetic analyses (ITS, GAPDH, CHS-1, HIS3, ACT, TUB2) of 102 strains previously identified as Ga. glycines, C. magnum and C. orchidearum as well as other related strains from different culture collections and studies placed these taxa in three species complexes, and distinguished at least 24 species, including 11 new species. In this study, C. magnum, C. orchidearum and C. piperis were epitypified and their taxonomy resolved, while C. cliviicola was proposed as a new name for C. cliviae. Furthermore, a sexual morph was observed for C. yunnanense, while C. brevisporum, C. cliviicola and C. tropicicola were reported from new hosts or countries. Regarding their conidial morphology, species in the C. dracaenophilum, C. magnum and C. orchidearum species complexes are reminiscent of C. gloeosporioides or C. boninense s. lat., and were likely to be confused with them in the past.

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“…The C. fructicola species was originally reported to cause anthracnose on coffee fruit in Thailand (Prihastuti et al., ). This pathogen had been previously reported on Manihot sculenta, Nopalea cochenillifera , Annona muricata , Annona squamosal , Mangifera indica , Phaseolus lunatus and Malus domestica in Brazil (Bragança, Silva, Haddad, & Oliveira, ; Conforto, Lima, Garcete‐Gomez, Câmara, & Michereff, ; Costa et al., ; Lima et al., ; Sousa et al., ; Velho, Alaniz, Casanova, Mondino, & Stadnik, ).…”

Section: Discussionmentioning

confidence: 68%

Diversity of pathogenic and endophytic Colletotrichum isolates from Licania tomentosa in Brazil

et al. 2018

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Licania tomentosa (Chrysobalanaceae), also known as “oiti,” is a forest tree mainly used for urban afforestation in Brazil. Although anthracnose caused by Colletotrichum gloeosporioides is the main disease that threatens this tree, the identification of the species was based on morphological characteristics only. Owing to the need to use the molecular approach to pinpoint the identity of this pathogen with precision, the aim of this study was to identify endophytic and pathogenic Colletotrichum isolates from L. tomentosa based on both morphological and molecular data. For prior identification, partial sequences of the GAPDH region were obtained of all the 35 isolates (10 endophytic and 25 pathogenic). After analysis, ten isolates, representative of each clade, were selected for multilocus phylogenetic analysis (ACT, CAL, CHS‐1, GAPDH, TUB2, SOD2 and ITS). In addition, a tree based on the ApMat region was obtained for comparison with the multilocus tree. Morphological characterization (colony growth, conidial size and appressoria shape) was also performed for each species. To prove pathogenicity, L. tomentosa leaves were inoculated on the adaxial surface by mycelial plugs and conidial suspension. All isolates obtained belong to the Colletotrichum gloeosporioides complex. The Apmat tree has the same topology as the multilocus tree, allowing for the discrimination of the different species of Colletotrichum on L. tomentosa. Endophytic isolates of C. fructicola, C. queenslandicum, and C. siamense were acquired whereas pathogenic isolates were identified as C. siamense and C. tropicale, although all species were pathogenic on the wounded leaves of L. tomentosa. This is the first worldwide report of this Colletotrichum species associated with L. tomentosa and the first recording of C. queenslandicum in Brazil.

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Colletotrichum species causing anthracnose on lima bean in Brazil

Cited by 20 publications

References 34 publications

Biology, pathogenicity, and haplotype analyses of Colletotrichum cliviae: a novel soybean anthracnose agent in warm tropical areas

Biology, pathogenicity, and haplotype analyses of Colletotrichum cliviae: a novel soybean anthracnose agent in warm tropical areas

The Colletotrichum dracaenophilum, C. magnum and C. orchidearum species complexes

Diversity of pathogenic and endophytic Colletotrichum isolates from Licania tomentosa in Brazil

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